Chronic exposure to Δ9-tetrahydrocannabinol downregulates oxytocin and oxytocin-associated neurophysin in specific brain areas
Abstract
Cannabinoids are widely abused drugs. Our goal was to identify genes modulated by Delta9-tetrahydrocannabinol (Delta9-THC) treatment. We found that chronic administration of Delta9-THC (1.5 mg/kg/day, i.p.; 7 days) to rats, downregulates the expression of oxytocin-neurophysin (OT-NP) mRNA and of OT and oxytocin-associated NP (NPOT) immunoreactivity in nucleus accumbens (NAc) and ventral tegmental area (VTA), brain areas involved in reward and addiction. Real-time PCR revealed a 60% and 53% reduction of OT-NP mRNA in NAc and VTA, respectively, under chronic treatment, while no changes were observed in NAc after 24 h. Immunohistochemistry showed a large decrease in number of OT and NPOT-stained fibers in NAc (by 59% and 52%, respectively) and VTA (by 50% and 56%, respectively). No changes in cell staining were observed in the paraventricular nucleus and supraoptic nucleus. As OT is known to inhibit development of drug tolerance and attenuate withdrawal symptoms, we suggest that OT downregulation could play a role during the establishment of the chronic effects of Delta9-THC.
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Chronic delta-9-THC administration modulates oxytocin expression in the nucleus accumbens and ventral tegmental area.
“chronic delta-9-THC administration has been shown to modulate oxytocin expression in the nucleus accumbens and ventral tegmental area (Butovsky et al., 2006)”
From Effect of oxytocin on craving and stress response in marijuana-dependent individuals: A pilot study (2013) · cites this paper as Butovsky et al., 2006
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- Authors
- Butovsky, E., Juknat, A., Elbaz, J., Shabat-Simon, M., Eilam, R., Zangen, A., Altstein, M., & Vogel, Z.
- Affiliations
- Weizmann Institute of Science (IL)
- Agricultural Research Organization (IL)
- Corresponding author
- Vogel, Zvi
- Venue
- Molecular and Cellular Neuroscience
- Publisher
- Elsevier BV
- Published
- 2006-04
- Volume
- 31
- Issue
- 4
- Pages
- 795-804
- ISSN
- 1044-7431 (ISSN-L)
- OpenAlex
- W1989197363
- Cited by
- 52
- References
- 82
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 5 · 2019: 1 · 2018: 2 · 2017: 5 · 2016: 5 · 2015: 4 · 2014: 2 · 2013: 4
- FWCI
- 1.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Molecular and Cellular Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Drug Abuse
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- delta-9-tetrahydrocannabinol, oxytocin, neurophysin, nucleus accumbens, ventral tegmental area, gene downregulation, reward, drug addiction, paraventricular nucleus, supraoptic nucleus
- MeSH terms
- Animals, Brain, Hallucinogens, Male, Neurophysins, Oxytocin, RNA, Messenger, Dronabinol, Rats, Sprague-Dawley, Rats
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