Subfields of the hippocampal formation at 7 T MRI: In vivo volumetric assessment
Abstract
Animal and human autopsy studies suggest that subfields of the hippocampal formation are differentially affected by neuropsychiatric diseases. Therefore, subfield volumes may be more sensitive to effects of disease processes. The few human studies that segmented subfields of the hippocampal formation in vivo either assessed the subfields only in the body of the hippocampus, assessed only three subfields, or did not take the differential angulation of the head of the hippocampus into account. We developed a protocol using 7 Tesla MRI with isotropic voxels to reliably delineate the entorhinal cortex (ERC), subiculum (SUB), CA1, CA2, CA3, dentate gyrus (DG)&CA4 along the full-length of the hippocampus. Fourteen subjects (aged 54-74 years, 2 men and 12 women) were scanned with a 3D turbo spin echo (TSE) sequence with isotropic voxels of 0.7 × 0.7 × 0.7 mm(3) on a 7 T MRI whole body scanner. Based on previous protocols and extensive anatomic atlases, a new protocol for segmentation of subfields of the hippocampal formation was formulated. ERC, SUB, CA1, CA2, CA3 and DG&CA4 were manually segmented twice by one rater from coronal MR images. Good-to-excellent consistency was found for all subfields (Intraclass Correlation Coefficient's (ICC) varying from 0.74 to 0.98). Accuracy as measured with the Dice Similarity Index (DSI) was above 0.82 for all subfields, with the exception of the smaller subfield CA3 (0.68-0.70). In conclusion, this study shows that it is possible to delineate the main subfields of the hippocampal formation along its full-length in vivo at 7 T MRI. Our data give evidence that this can be done in a reliable manner. Segmentation of subfields in the full-length of the hippocampus may bolster the study of the etiology neuropsychiatric diseases.
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Hippocampal subfield volumetry is a promising biomarker for early AD detection at a predementia or even presymptomatic stage.
“They suggest that hippocampal subfield volumetry is a promising biomarker for early AD detection at a predementia or even presymptomatic stage”
From Hippocampal subfield volumetry in mild cognitive impairment, Alzheimer's disease and semantic dementia (2013) · cites this paper as Wisse et al., 2012
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Self-citation
- Authors
- Wisse, L. E. M., Gerritsen, L., Zwanenburg, J. J. M., Kuijf, H. J., Luijten, P. R., Biessels, G. J., & Geerlings, M. I.
- Affiliations
- University Medical Center Utrecht (NL)
- Karolinska Institutet (SE)
- Corresponding author
- Geerlings, M.I.
- ORCID
- Venue
- NeuroImage
- Publisher
- Elsevier BV
- Published
- 2012-07
- Volume
- 61
- Issue
- 4
- Pages
- 1043-1049
- ISSN
- 1053-8119 (ISSN-L)
- OpenAlex
- W2023253889
- Cited by
- 183
- References
- 43
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 9 · 2023: 9 · 2022: 7 · 2021: 6 · 2020: 13 · 2019: 9 · 2018: 18 · 2017: 18 · 2016: 26 · 2015: 23 · 2014: 22 · 2013: 10 · 2012: 2
- FWCI
- 18.77
- Type
- journal article
- Language
- en
- Update notices
- erratum (2016-01-15)
- Open access
- closed
- Locations
- NeuroImage (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Netherlands Organization for Scientific Research (917-66-311)
- ITEA (Information Technology for European Advancement)
- Topics
- Physical Sciences › Computer Science › Computer Vision and Pattern Recognition › Medical Image Segmentation Techniques
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Keywords
- hippocampal subfields, hippocampal formation, 7T MRI, entorhinal cortex, subiculum, CA1, CA2, CA3, dentate gyrus, CA4, manual segmentation, inter-rater reliability, isotropic voxels, neuropsychiatric disorders
- MeSH terms
- Aged, Female, Hippocampus, Humans, Image Interpretation, Computer-Assisted, Magnetic Resonance Imaging, Male, Middle Aged
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