Metabolomic Disorders Unveil Hepatotoxicity of Environmental Microplastics in Wild Fish Serranus scriba (Linnaeus 1758)
Abstract
Coastal areas are worldwide subject to large inputs of anthropogenic wastes that are discharged directly into inshore waters, where they will be weathered into small microplastics (MPs) of up to a size <20 μm. This study provides information about the presence of small environmental MPs (≤3 μm) in the liver of adult benthopelagic fish Serranus scriba (Linnaeus 1758), caught from three coastal regions in Tunisia distinguished by different patterns of human activity. Polymer composition in fish liver was identified using Raman microspectroscopy. Results revealed differences in the abundance, size distribution and presence of plastic additives over the investigated sites. Polyethylene-vinyl acetate (PEVA: 34% particles/g of tissue), high density polyethylene (HDPE: 24.4%) and the two smaller size classes, i.e. 3-1.2 μm and 1.2-0.45 μm, were the most abundant MPs types and size distribution found, respectively, in Bizerte channel (BC) site (Bizerte city, Tunisia). Moreover, at hepatic level data showed a significant site-dependent cytotoxicity expressed by changes in malondialdehyde (MDA) content, presence of reactive oxygen species (ROS) expressed by altered level of catalase (CAT) and glutathione-S-transferase (GST) activities and in the content of metallothioneins (MTs), as well as genotoxicity by changes in the amount of micronucleus (MN), and neurotoxicity by altered activity of acetylcholinesterase (AChE). A innovative metabolomics analysis was also performed to further investigate the distinct patterns of key metabolite changes in the liver of Serranus scriba. A total of 36 metabolites were significantly affected, mainly involved in energy, amino acid and osmolyte metabolism. These findings emphasised for the first time a close relationship between the source, abundance and size ranges of environmental MPs ≤ 3 μm and their hepatotoxicity in wild organisms.
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Zitouni et al. pointed out significant site-dependent cytotoxicity in relation to MPs ingestion in Serranus scriba liver.
“Significant site-dependent cytotoxicity in relation to MPs’ ingestion was pointed out [43].”
From Is Wild Marine Biota Affected by Microplastics? (2022) · cites this paper as [43]
Neurotoxicity by altered activity of acetylcholinesterase was reported in Serranus scriba.
“Neurotoxicity by altered activity of acetylcholinesterase was also reported in Serranus scriba [43].”
From Is Wild Marine Biota Affected by Microplastics? (2022) · cites this paper as [43]
Zitouni et al. reported significant site-dependent genotoxicity by changes in the amount of micronucleus in the liver of Serranus scriba.
“Authors reported significant site-dependent genotoxicity by changes in the amount of micronucleus (see Table 1).”
From Is Wild Marine Biota Affected by Microplastics? (2022) · cites this paper as [43]
Zitouni et al. found that 36 metabolites in Serranus scriba liver, mainly involved in energy, amino acid, and osmolyte metabolism, were significantly affected by the presence of MPs.
“Zitouni et al. [43] performed an innovative metabolomic analysis on 36 metabolites in Serranus scriba liver. The metabolites investigated, mainly involved in energy, amino acid, and osmolyte metabolism, were significantly affected by the presence of MPs.”
From Is Wild Marine Biota Affected by Microplastics? (2022) · cites this paper as [43]
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Food chain microplastics contamination and impact on human health: a review
Eze, C. G., Nwankwo, C. E. I., Dey, S., Sundaramurthy, S., & Okeke, E. S. (2024) · Environmental Chemistry Letters · doi:10.1007/s10311-024-01734-2 · Cited by 192 · see citing works
Wang, W., Mao, X., Zhang, R., Zhou, X.-X., Liu, Y., Zhou, H., Jia, J., & Yan, B. (2023) · Environmental Science & Technology · doi:10.1021/acs.est.3c02769 · Cited by 106 · see citing works
Wang, S., Chen, L., Xu, S., Wang, Y., & Xu, S. (2023) · Environmental Pollution · doi:10.1016/j.envpol.2023.121388 · Cited by 96 · see citing works
Hao, Y., Sun, Y., Li, M., Fang, X., Wang, Z., Zuo, J., & Zhang, C. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2022.159270 · Cited by 89 · see citing works
Ali, S. S., Alsharbaty, M. H. M., Al-Tohamy, R., Schagerl, M., Al-Zahrani, M., Kornaros, M. E., & Sun, J. (2024) · Environmental Chemistry and Ecotoxicology · doi:10.1016/j.enceco.2024.12.005 · Cited by 77 · see citing works
Microplastics as a Threat to Aquatic Ecosystems and Human Health
Witczak, A., Przedpełska, L., Pokorska‐Niewiada, K., & Cybulski, J. (2024) · Toxics · doi:10.3390/toxics12080571 · Cited by 70 · see citing works
Najahi, H., Alessio, N., Squillaro, T., Conti, G. O., Ferrante, M., Bernardo, G. D., Galderisi, U., Messaoudi, I., Minucci, S., & Bannı, M. (2022) · Environmental Research · doi:10.1016/j.envres.2022.114088 · Cited by 65 · see citing works
Barboza, L. G. A., Otero, X. L., Fernández, E. V., Vieira, L. R., Fernandes, J. O., Cunha, S. C., & Guilhermino, L. M. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e13070 · Cited by 62 · see citing works
Tang, R., Ying, M., Luo, Y., El‐Naggar, A., Palansooriya, K. N., Sun, T., Cao, Y., Diao, Z., Zhang, Y., Lian, Y., et al. (2023) · Environmental Pollution · doi:10.1016/j.envpol.2023.121020 · Cited by 57 · see citing works
Is Wild Marine Biota Affected by Microplastics?
Porcino, N., Bottari, T., & Mancuso, M. (2022) · Animals · doi:10.3390/ani13010147 · Cited by 55 · see citing works
“[43] performed an innovative metabolomic analysis on 36 metabolites in Serranus scriba liver.”
BackgroundMethodInfluentialHazards of microplastics exposure to liver function in fishes: A systematic review and meta-analysis
Sun, Y., Deng, Q., Zhang, Q., Zhou, X., Chen, R., Li, S., Wu, Q., & Chen, H. (2024) · Marine Environmental Research · doi:10.1016/j.marenvres.2024.106423 · Cited by 53 · see citing works
Krikech, I., Conti, G. O., Pulvirenti, E., Rapisarda, P., Castrogiovanni, M., Maisano, M. S., Pennec, G. L., Leermakers, M., Ferrante, M., Cappello, T., et al. (2023) · Environmental Research · doi:10.1016/j.envres.2023.116608 · Cited by 49 · see citing works
A systematic review of the potential neurotoxicity of micro-and nanoplastics: the known and unknown
Vojnits, K., de León, A., Gibon, J., Barker, P. A., Mahmoudi, M., & Pakpour, S. (2025) · Particle and Fibre Toxicology · doi:10.1186/s12989-025-00647-4 · Cited by 46 · see citing works
Li, M., Hou, Z., Meng, R., Hao, S., & Wang, B. (2022) · Environment International · doi:10.1016/j.envint.2022.107644 · Cited by 46 · see citing works
Eliso, M. C., Billè, B., Cappello, T., & Maisano, M. S. (2024) · Fishes · doi:10.3390/fishes9030098 · Cited by 44 · see citing works
Marco, G. D., Eliso, M. C., Conti, G. O., Galati, M., Billè, B., Maisano, M. S., Ferrante, M., & Cappello, T. (2023) · Aquatic Toxicology · doi:10.1016/j.aquatox.2023.106736 · Cited by 42 · see citing works
Impact of Plastic Pollution on Marine Biodiversity in Italy
Bottari, T., Mghili, B., Gunasekaran, K., & Mancuso, M. (2024) · Water · doi:10.3390/w16040519 · Cited by 42 · see citing works
Abouda, S., Missawi, O., Cappello, T., Boughattas, I., Marco, G. D., Maisano, M. S., & Bannı, M. (2022) · Environmental Pollution · doi:10.1016/j.envpol.2022.119856 · Cited by 40 · see citing works
Birds as Bioindicators: Revealing the Widespread Impact of Microplastics
Carrasco, L., Jiménez-Mora, E., Utrilla, M. J., Pizarro, I. T., Reglero, M. M., Román, L. R.-S., & Martín‐Maldonado, B. (2025) · Birds · doi:10.3390/birds6010010 · Cited by 38 · see citing works
Bilal, M., Yaqub, A., Hassan, H. U., Akhtar, S., Rafiq, N., Shah, M. I. A., Hussain, I., Khan, M. S., Nawaz, A., Manoharadas, S., et al. (2023) · Toxics · doi:10.3390/toxics11120972 · Cited by 37 · see citing works
Ferreira, O., Barboza, L. G. A., Rudnitskaya, A. M., Moreirinha, C., Vieira, L. R., Botelho, M. J., Vale, C., Fernandes, J. O., Cunha, S. C., & Guilhermino, L. M. (2023) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2023.115704 · Cited by 33 · see citing works
Sidira, M., Agriopoulou, S., Smaoui, S., & Varzakas, T. H. (2024) · Applied Sciences · doi:10.3390/app142210755 · Cited by 30 · see citing works
Lomonaco, T., Persiani, E., Biagini, D., Gisone, I., Ceccherini, E., Cecchettini, A., Corti, A., Ghimenti, S., Francesco, F. D., Castelvetro, V., et al. (2024) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2024.116695 · Cited by 23 · see citing works
Noureen, A., Marco, G. D., Rehman, N., Jabeen, F., & Cappello, T. (2022) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph192013462 · Cited by 22 · see citing works
Gopal, J., Sivanesan, I., Muthu, M., & Oh, J. (2022) · Foods · doi:10.3390/foods11243976 · Cited by 22 · see citing works
Abouda, S., Galati, M., Conti, G. O., Cappello, T., Abelouah, M. R., Romdhani, I., Alla, A. A., Ferrante, M., Maisano, M. S., & Bannı, M. (2024) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2024.135404 · Cited by 20 · see citing works
Cohen-Sánchez, A., Solomando, A., Pinya, S., Tejada, S., Valencia, J. M., Box, A., & Sureda, A. (2023) · Toxics · doi:10.3390/toxics11040365 · Cited by 19 · see citing works
Δίγκα, Ν., Patsiou, D., Kaberi, H., Krasakopoulou, Ε., & Tsangaris, C. (2023) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2023.115613 · Cited by 21 · see citing works
Rashid, E., Hussain, S. M., Sarker, P. K., Ali, S., & Paray, B. A. (2024) · Aquaculture Reports · doi:10.1016/j.aqrep.2024.102100 · Cited by 20 · see citing works
RetractedZhang, L., García-Pérez, P., Muñoz-Palazón, B., González‐Martínez, A., Lucini, L., & Rodríguez–Sánchez, A. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.172915 · Cited by 19 · see citing works
Mansuri, A., Trivedi, C., & Kumar, A. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.177510 · Cited by 18 · see citing works
Hattab, S., Cappello, T., Boughattas, I., Sassı, K., Mkhinini, M., Zitouni, N., Missawi, O., Eliso, M. C., Znaïdi, A., & Bannı, M. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.172975 · Cited by 18 · see citing works
Marco, G. D., Afsa, S., Galati, M., Billè, B., Parrino, V., Mansour, H. B., & Cappello, T. (2022) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2022.103888 · Cited by 18 · see citing works
Ma, L., Lu, J., Yao, T., Ye, L., & Wang, J. (2022) · Antioxidants · doi:10.3390/antiox11061178 · Cited by 17 · see citing works
Conger, E., Dziobak, M. K., McCabe, E. J. B., Curtin, T., Gaur, A., Wells, R. S., Weinstein, J. E., & Hart, L. B. (2024) · Environments · doi:10.3390/environments11090185 · Cited by 16 · see citing works
Synergistic effects between microplastics and glyphosate on honey bee larvae
Mitton, G., Corona, M., Alburaki, M., Iglesias, A., Ramos, F., Fuentes, G., Vázquez, M. M., Mitton, F. M., Chan, P., Ruffinengo, S. R., et al. (2024) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2024.104550 · Cited by 16 · see citing works
Afsa, S., Marco, G. D., Cristaldi, A., Giannetto, A., Galati, M., Billè, B., Conti, G. O., Mansour, H. B., Ferrante, M., & Cappello, T. (2023) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2023.104167 · Cited by 16 · see citing works
Polystyrene nanoplastics exposure causes erectile dysfunction in rats
Wang, M., Dai, B., Liu, Q., Wang, X., Xiao, Y., Zhang, G., Jiang, H., Zhang, X., & Zhang, L. (2024) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2024.116551 · Cited by 16 · see citing works
Laganà, A., Billè, B., Visalli, G., Facciolà, A., Cappello, T., Maisano, M. S., & Pietro, A. D. (2025) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2025.179262 · Cited by 16 · see citing works
Marco, G. D., Billè, B., Brandão, F., Galati, M., Pereira, P., Cappello, T., & Pacheco, M. (2023) · Toxics · doi:10.3390/toxics11020181 · Cited by 15 · see citing works
Missawi, O., Wouters, C., Lambert, J., Garigliany, M.-M., Kestemont, P., & Cornet, V. (2024) · Chemosphere · doi:10.1016/j.chemosphere.2023.141107 · Cited by 15 · see citing works
Djekoun, M., Gaaied, S., Romdhani, I., Abelouah, M. R., Missaoui, Y., Boubekeur, M. S., Trea, F., Lakbar, C., Ouali, K., & Bannı, M. (2024) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2024.116705 · Cited by 15 · see citing works
Eliso, M. C., Billè, B., Marco, G. D., Pulvirenti, E., Bello, F. D., Rapisarda, P., Pereira, P., Galati, M., Conti, G. O., Ferrante, M., et al. (2025) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2025.140139 · Cited by 13 · see citing works
Lourenço, S. C., Aleluia, A., Barboza, L. G. A., Otero, X. L., Cunha, S. C., Fernandes, J. O., & Guilhermino, L. M. (2025) · Marine Environmental Research · doi:10.1016/j.marenvres.2025.107139 · Cited by 12 · see citing works
Santos, M. E. S., Horký, P., Grabicová, K., Steinbach, C., Hubená, P., Šálková, E., Slavík, O., Grabic, R., & Randák, T. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.163167 · Cited by 12 · see citing works
Afsa, S., Maisano, M. S., Germanà, A., & Cappello, T. (2025) · Environmental Research · doi:10.1016/j.envres.2025.122236 · Cited by 11 · see citing works
Tovar-Salvador, M. L., Quintero, R. R., Pintado‐Herrera, M. G., Miñambres, M. P., & Lara‐Martín, P. A. (2024) · Marine Environmental Research · doi:10.1016/j.marenvres.2024.106893 · Cited by 10 · see citing works
Zheng, Y., Li, J., Gao, J., Jin, W., Hu, J., Sun, Y., Zhu, H., & Xu, G. (2024) · Comparative Biochemistry and Physiology Part D Genomics and Proteomics · doi:10.1016/j.cbd.2024.101370 · Cited by 10 · see citing works
Microplastics and ecosystem services: persistence, disruption mechanisms, and mitigation strategies
Gokila, B., Duraisamy, J., Guruanand, C., & Arumugam, R. D. (2025) · Environmental Reviews · doi:10.1139/er-2025-0080 · Cited by 8 · see citing works
The potential of microplastics acting as vector for triclosan in aquatic environments
Chand, N., Krause, S., & Prajapati, S. K. (2025) · Aquatic Toxicology · doi:10.1016/j.aquatox.2025.107381 · Cited by 9 · see citing works
Martins, A., Barboza, L. G. A., Vieira, L. R., Botelho, M. J., Vale, C., & Guilhermino, L. M. (2025) · Marine Environmental Research · doi:10.1016/j.marenvres.2024.106925 · Cited by 7 · see citing works
Keang, K. S., Sorn, S., Cheng, S., Wasnik, S., Zhang, H., & Cross, J. S. (2025) · Environmental Pollution · doi:10.1016/j.envpol.2025.127141 · Cited by 6 · see citing works
Kono, K., Ito, M., Hano, T., & Ohkubo, N. (2024) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2024.117216 · Cited by 6 · see citing works
Laganà, A., Billè, B., Pietro, A. D., Facciolà, A., Celesti, C., Iannazzo, D., Galati, M., Maisano, M. S., Cappello, T., & Visalli, G. (2026) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2026.141239 · Cited by 5 · see citing works
Balcıoğlu, E. B. (2025) · Sustainability · doi:10.3390/su17104731 · Cited by 6 · see citing works
Neurophysiological and Behavioral Effects of Micro- and Nanoplastics in Aquatic Organisms
Belanger, R. M., & Storks, L. (2026) · Animals · doi:10.3390/ani16060941 · Cited by 4 · see citing works
Belgacem, R., Zitouni, N., Khazri, A., Abidi, O., Alzwawy, A. G., Béjaoui, M., Bouleefah, F., Bannı, M., & Mahmoudi, E. (2025) · Environmental Toxicology and Chemistry · doi:10.1093/etojnl/vgae023 · Cited by 4 · see citing works
Sellami, B., Nasri‐Ammar, K., Fkiri, A., Boughanmi, F., Beyrem, H., & Jelassi, R. (2024) · Chemistry and Ecology · doi:10.1080/02757540.2024.2366375 · Cited by 4 · see citing works
Unleashing multi-omic approaches to address environmental microplastic hazards in marine polychaetes
Missawi, O., Vaccari, F., Zhang, L., Miras‐Moreno, B., Boughattas, I., Bandini, F., Lucini, L., Puglisi, E., & Bannı, M. (2025) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2025.118345 · Cited by 3 · see citing works
The Importance of NMR as a Discovery Tool
Downey, K., Ronda, K., Steiner, K., Jobst, K. J., Kleywegt, S. M., Simpson, M. J., & Simpson, A. J. (2024) · Royal Society of Chemistry eBooks · doi:10.1039/bk9781837671250-00010 · Cited by 2 · see citing works
Supriadi, A., Saputra, D., Lestari, S. D., Rachmawati, S., & Ridhowati, S. (2025) · Tropical Journal of Natural Product Research · doi:10.26538/tjnpr/v9i7.38 · Cited by 2 · see citing works
James, K., Gopinath, V., Vasant, K., & Devipriya, S. (2026) · Estuarine, Coastal and Shelf Science · doi:10.1016/j.ecss.2026.110055 · Cited by 2 · see citing works
Microplastics in the Mediterranean Biota
Mancuso, M., Porcino, N., Blasco, J., Romeo, T., Savoca, S., Spanò, N., & Bottari, T. (2023) · SpringerBriefs in environmental science · doi:10.1007/978-3-031-30481-1_2
Micro/nanoplastic threats to human and animal health: mitigation strategies with melatonin
Reiter, R. J., Sharma, R., Chuffa, L. G. D. A., Zuccari, D. A. P. D. C., Loh, D., & Bannı, M. (2026) · Frontiers in Environmental Science · doi:10.3389/fenvs.2026.1834609
Atamanalp, M., Köktürk, M., Yeltekin, A. Ç., Firidin, Ş., Uçar, A., Gündüz, F., Parlak, V., Koşar, K., Gün, A., Özçelik, E. Z., et al. (2026) · Fish Physiology and Biochemistry · doi:10.1007/s10695-026-01760-7
Atri, A., Sharma, M., Rana, V., Singh, V., Sonia, N., Themchuirin, L., Yadav, A., Chaurasia, M., Yadav, P., Dolkar, P., et al. (2026) · Environmental Monitoring and Assessment · doi:10.1007/s10661-026-15818-9
- Authors
- Zitouni, N., Cappello, T., Missawi, O., Boughattas, I., De Marco, G., Belbekhouche, S., Mokni, M., Alphonse, V., Guerbej, H., Bousserrhine, N., & Banni, M.
- Affiliations
- University of Monastir (TN)
- University of Messina (IT)
- University of Sousse (TN)
- Centre National de la Recherche Scientifique (FR)
- Hôpital Farhat Hached (TN)
- Université Paris-Est Créteil (FR)
- Corresponding author
- Cappello, Tiziana
- Venue
- Science of The Total Environment
- Publisher
- Elsevier BV
- Published
- 2022-09
- Volume
- 838
- Issue
- Pt 1
- Pages
- 155872
- ISSN
- 0048-9697 (ISSN-L)
- OpenAlex
- W4280496553
- Cited by
- 66
- References
- 91
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 23 · 2023: 14 · 2022: 9
- FWCI
- 3.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- The Science of The Total Environment (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Ministry of Higher Education and Scientific Research (LR21AGR02)
- Centre National de la Recherche Scientifique
- Topics
- Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
- Keywords
- microplastics, hepatotoxicity, Serranus scriba, fish liver, metabolomics, ethylene-vinyl acetate copolymer, high-density polyethylene, polymer additives, oxidative stress, genotoxicity, neurotoxicity, metallothionein, coastal pollution
- MeSH terms
- Microplastics, Acetylcholinesterase, Animals, Bass, Environmental Monitoring, Plastics, Water Pollutants, Chemical, Polyethylene, Metabolomics, Chemical and Drug Induced Liver Injury
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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