Cited by 131Mitochondrial Function and PathologyRedox biology and oxidative stressATP Synthase and ATPases Research

Production of endogenous matrix superoxide from mitochondrial complex I leads to activation of uncoupling protein 3

Talbot, Darren A, Lambert, Adrian J, Brand, Martin D·2004·FEBS Letters

Abstract

Superoxide generated using exogenous xanthine oxidase indirectly activates an uncoupling protein (UCP)‐mediated proton conductance of the mitochondrial inner membrane. We investigated whether endogenous mitochondrial superoxide production could also activate proton conductance. When respiring on succinate, rat skeletal muscle mitochondria produced large amounts of matrix superoxide. Addition of GDP to inhibit UCP3 markedly inhibited proton conductance and increased superoxide production. Both superoxide production and the GDP‐sensitive proton conductance were suppressed by rotenone plus an antioxidant. Thus, endogenous superoxide can activate the proton conductance of UCP3, which in turn limits mitochondrial superoxide production. These observations provide a departure point for studies under more physiological conditions.

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Crossref · 10.1016/s0014-5793(03)01386-3

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Authors
Talbot, D. A., Lambert, A. J., & Brand, M. D.
Affiliations
MRC Mitochondrial Biology Unit (GB)
Corresponding author
Brand, Martin D
Venue
FEBS Letters
Publisher
Wiley
Published
2003-12-04
Volume
556
Issue
1-3
Pages
111-115
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W2052622056
Cited by
131
References
29
Citations by year
2026: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 1 · 2018: 6 · 2016: 4 · 2015: 1 · 2014: 5 · 2013: 2 · 2012: 7
FWCI
4.94
Type
journal article
Language
en
Open access
closed
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
Keywords
mitochondrial superoxide, complex I, UCP3, proton conductance, skeletal muscle mitochondria, GDP inhibition, rotenone, antioxidants, succinate respiration, mitochondrial reactive oxygen species
MeSH terms
Uncoupling Protein 3, Animals, Carrier Proteins, Guanosine Diphosphate, Hydrogen Peroxide, Ion Channels, Kinetics, Male, Membrane Potentials, Mitochondria, Muscle, Oxygen Consumption, Protons, Rotenone, Superoxides, Vitamin E, Rats, Wistar, Succinic Acid, Mitochondrial Proteins, Electron Transport Complex I, Rats

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