Cited by 472Nitric Oxide and Endothelin EffectsRedox biology and oxidative stressNeutrophil, Myeloperoxidase and Oxidative Mechanisms

What nitrates tyrosine? Is nitrotyrosine specific as a biomarker of peroxynitrite formation in vivo?

Halliwell, Barry·1997·FEBS Letters

Abstract

Peroxynitrite (ONOO-) is a 'reactive nitrogen species' that can be formed (among other reactions) by combination of superoxide (O2.-) and nitric oxide (NO.) radicals. It is being increasingly proposed as a contributor to tissue injury in several human diseases. The evidence presented for peroxynitrite participation usually includes the demonstration of increased nitrotyrosine levels in the injured tissue. Indeed, this is often the only evidence presented: the assumption is that formation of nitrotyrosine is a biomarker specifically diagnostic of ONOO- production. The present article examines this assumption and concludes that nitrotyrosine is a biomarker for 'nitrating species' rather than being specific for ONOO-.

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Crossref · 10.1016/s0014-5793(97)00469-9

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85 of 85 references verified0 citation errors · 0 not checkable

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  • Peroxynitrite is formed by phagocytes to kill invading microorganisms, but at high concentrations can oxidise host biomolecules by nitration, causing cellular damage responsible for diverse diseases.

    “Peroxynitrite is formed by phagocytes for killing invading microorganisms, nevertheless if in high concentrations can oxidise several host biomolecules by nitration with the consequent cellular damages being responsible for diverse diseases [26].”

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Authors
Halliwell, B.
Affiliations
King's College London (GB)
Corresponding author
Halliwell, Barry
Venue
FEBS Letters
Publisher
Wiley
Published
1997-07-14
Volume
411
Issue
2-3
Pages
157-160
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W1974185399
Cited by
472
References
85
Citations by year
2026: 3 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 8 · 2021: 8 · 2020: 6 · 2019: 5 · 2018: 13 · 2017: 5 · 2016: 14 · 2015: 6 · 2014: 10 · 2013: 9 · 2012: 20
FWCI
16.32
Type
journal article
Language
en
Open access
closed
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
nitrotyrosine, peroxynitrite, reactive nitrogen species, nitric oxide, superoxide, biomarkers, tissue damage, nitration
MeSH terms
Free Radicals, Humans, Peroxidase, Nitrates, Nitric Oxide, Nitrogen Dioxide, Nitrous Acid, Superoxides, Tyrosine, Molecular Structure, Biomarkers

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