Effects of oxidative and nitrosative stress in brain on p53 proapoptotic protein in amnestic mild cognitive impairment and Alzheimer disease
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Many studies reported that oxidative and nitrosative stress might be important for the pathogenesis of Alzheimer's disease (AD) beginning with arguably the earliest stage of AD, i.e., as mild cognitive impairment (MCI). p53 is a proapoptotic protein that plays an important role in neuronal death, a process involved in many neurodegenerative disorders. Moreover, p53 plays a key role in the oxidative stress-dependent apoptosis. We demonstrated previously that p53 levels in brain were significantly higher in MCI and AD IPL (inferior parietal lobule) compared to control brains. In addition, we showed that in AD IPL, but not in MCI, HNE, a lipid peroxidation product, was significantly bound to p53 protein. In this report, we studied by means of immunoprecipitation analysis, the levels of markers of protein oxidation, 3-nitrotyrosine (3-NT) and protein carbonyls, in p53 in a specific region of the cerebral cortex, namely the inferior parietal lobule, in MCI and AD compared to control brains. The focus of these studies was to measure the oxidation and nitration status of this important proapoptotic protein, consistent with the hypothesis that oxidative modification of p53 could be involved in the neuronal loss observed in neurodegenerative conditions.
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p53 is significantly increased in MCI and AD IPL with p53 significantly modified by protein-bound HNE, 3-NT, and carbonyls in an AD brain.
“p53 is significantly increased in MCI and AD IPL with p53 significantly modified by protein-bound HNE, 3-NT, and carbonyls in an AD brain.”
From Mitochondrial Oxidative and Nitrosative Stress and Alzheimer Disease (2020) · cites this paper as [87]
p53 is significantly modified by carbonylation, but not 3NT or HNE in MCI IPL.
“p53 is significantly modified by carbonylation, but not 3NT or HNE in MCI IPL [87,88].”
From Mitochondrial Oxidative and Nitrosative Stress and Alzheimer Disease (2020) · cites this paper as [87]
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- Authors
- Cenini, G., Sultana, R., Memo, M., & Butterfield, D. A.
- Affiliations
- University of Kentucky (US)
- University of Brescia (IT)
- Corresponding author
- Butterfield, D. Allan
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 45
- Issue
- 1
- Pages
- 81-85
- ISSN
- 0891-5849 (ISSN-L)
- PMC
- PMC2587421
- OpenAlex
- W1989114735
- Cited by
- 79
- References
- 47
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 3 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 5 · 2019: 1 · 2018: 6 · 2016: 3 · 2015: 5 · 2014: 4 · 2013: 5 · 2012: 8
- FWCI
- 5.19
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Institutional Research Information System (Università degli Studi di Brescia) (submittedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (P01AG010836, P01AG005119, R01AG029839, AG029839, AG 10836, AG0 5119)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- p53, oxidative stress, nitrosative stress, amnestic mild cognitive impairment, Alzheimer's disease, inferior parietal lobule, nitrotyrosine, protein carbonylation, lipid peroxidation, neuronal apoptosis, protein oxidation, neurodegeneration
- MeSH terms
- Aged, 80 and over, Alzheimer Disease, Amnesia, Brain, Cognition Disorders, Female, Humans, Male, Nitrogen, Oxidation-Reduction, Protein Binding, Tyrosine, Tumor Suppressor Protein p53, Apoptosis, Oxidative Stress
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