Cited by 155Cannabis and Cannabinoid ResearchPain Mechanisms and TreatmentsIon Channels and Receptors

Characterization of Δ9-Tetrahydrocannabinol and Anandamide Antinociception in Nonarthritic and Arthritic Rats

Smith, Forrest L, Fujimori, Ken, Lowe, John, Welch, Sandra P·1998·Pharmacology Biochemistry and Behavior

Abstract

Little is known about the effectiveness of delta9-tetrahydrocannabinol (THC) and anandamide in blocking mechanical nociception. Even less is known about their antinociceptive efficacy in chronic inflammatory arthritis induced by Freund's complete adjuvant. The hypothesis was tested that THC and anandamide elicit antinociception in the paw pressure test, and that arthritic rats would exhibit a different response. In nonarthritic rats, THC- and anandamide-induced antinociception lasted 90 min and 15 min, respectively, while antinociception lasted 90 min and 30 min, respectively, in arthritic rats. Area under the curve calculations revealed no effect of arthritis on THC- and anandamide-induced antinociception. Another hypothesis was that paw pressure thresholds in arthritic rats reflect chronic cannabinoid receptor stimulation due to elevations in free anandamide levels. Yet, the CB1 receptor antagonist SR141716A failed to alter paw pressure thresholds in either nonarthritic or arthritic rats. Further investigation revealed that SR141716A significantly blocked THC antinociception, with no effect on anandamide. Thus, anandamide's effects did not result from CB1 receptor stimulation, and any potential contribution of endogenous anandamide in arthritis was not revealed. Finally, THC and anandamide appear to release an as yet unknown endogenous opioid, because naloxone significantly blocked their effects. This study indicates that anandamide and THC may act at different receptor sites to modulate endogenous opioid levels in mechanical nociception.

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Crossref · 10.1016/s0091-3057(97)00583-2

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Authors
Smith, F. L., Fujimori, K., Lowe, J., & Welch, S. P.
Affiliations
Virginia Commonwealth University Medical Center (US)
Corresponding author
Smith, Forrest L
Venue
Pharmacology Biochemistry and Behavior
Publisher
Elsevier BV
Published
1998-05
Volume
60
Issue
1
Pages
183-191
ISSN
0091-3057 (ISSN-L)
OpenAlex
W1968714225
Cited by
154
References
52
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 5 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 5
FWCI
8.78
Type
journal article
Language
en
Open access
closed
Locations
  • Pharmacology Biochemistry and Behavior (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute on Drug Abuse (P01DA009789, DA 05274, DA‐03672, DA 09789)
Topics
  • Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
  • Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
Keywords
delta-9-tetrahydrocannabinol, anandamide, antinociception, mechanical nociception, inflammatory arthritis, complete Freund's adjuvant, CB1 receptor, SR141716A, naloxone, endogenous opioids, cannabinoid receptors
MeSH terms
Rimonabant, Analgesics, Animals, Arachidonic Acids, Arthritis, Experimental, Cannabinoids, Male, Piperidines, Pyrazoles, Receptors, Drug, Dronabinol, Rats, Sprague-Dawley, Pain Threshold, Opioid Peptides, Receptors, Cannabinoid, Rats, Polyunsaturated Alkamides, Endocannabinoids

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