The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Microarray Identification of FMRP-Associated Brain mRNAs and Altered mRNA Translational Profiles in Fragile X Syndrome
Open accessAbstract
Fragile X syndrome results from the absence of the RNA binding FMR protein. Here, mRNA was coimmunoprecipitated with the FMRP ribonucleoprotein complex and used to interrogate microarrays. We identified 432 associated mRNAs from mouse brain. Quantitative RT-PCR confirmed some to be >60-fold enriched in the immunoprecipitant. In parallel studies, mRNAs from polyribosomes of fragile X cells were used to probe microarrays. Despite equivalent cytoplasmic abundance, 251 mRNAs had an abnormal polyribosome profile in the absence of FMRP. Although this represents <2% of the total messages, 50% of the coimmunoprecipitated mRNAs with expressed human orthologs were found in this group. Nearly 70% of those transcripts found in both studies contain a G quartet structure, demonstrated as an in vitro FMRP target. We conclude that translational dysregulation of mRNAs normally associated with FMRP may be the proximal cause of fragile X syndrome, and we identify candidate genes relevant to this phenotype.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“We can thus conclude that the co-repressor activity of CYFIP1-FMRP does not affect G-quadruplex-containing mRNAs, which represent a subset of FMRP targets (Brown et al., 2001; Maurin et al., 2014; Suhl et al., 2015).”
From New insights into the regulatory function of CYFIP1 in the context of WAVE- and FMRP-containing complexes (2017) · cites this paper as Brown et al., 2001
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“G-quadruplex mRNA is a structure bound by FMRP harbored by some of its critical targets (MAP1B, PP2AC, FMR1, SHANKL, SEMA3F) (Brown et al., 2001; Castets et al., 2005; Schaeffer et al., 2001; Zhang et al., 2014; Menon and Mihailescu, 2007).”
From New insights into the regulatory function of CYFIP1 in the context of WAVE- and FMRP-containing complexes (2017) · cites this paper as Brown et al., 2001
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Moreover, the G-quadruplex/FMRP complex mediates the repression activity of FMRP in translational regulation (Schaeffer et al., 2001; Brown et al., 2001; Castets et al., 2005; Melko and Bardoni, 2010; Suhl et al., 2015).”
From New insights into the regulatory function of CYFIP1 in the context of WAVE- and FMRP-containing complexes (2017) · cites this paper as Brown et al., 2001
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“In contrast to NR2A and NR2B, the NR1 mRNA was not associated with FMRP by the coimmunoprecipitation assay (Figure 8A), nor was there coprecipitation of GAPDH mRNAs, a commonly used negative control that is believed not to be regulated by FMRP (Brown et al., 2001).”
From Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132 (2010) · cites this paper as Brown et al., 2001
The cited source says what the claim attributes to it.
In the citing paper
“We followed the standard procedure for FMRP immunoprecipitation used to identify mRNA targets (Brown et al., 2001).”
From Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132 (2010) · cites this paper as Brown et al., 2001
The check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“In contrast to NR2A and NR2B, the NR1 mRNA was not associated with FMRP by the coimmunoprecipitation assay (Figure 8A), nor was there coprecipitation of GAPDH mRNAs, a commonly used negative control that is believed not to be regulated by FMRP (Brown et al., 2001).”
From Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132 (2010) · cites this paper as Brown et al., 2001
Experimental — accuracy not yet measuredIn the citing paper
“Encoded by the FMR1 gene, Fragile X mental retardation protein (FMRP) mostly operates as an mRNA-binding protein (Fig. 5B2) that represses translation of an emerging subset of mRNAs (Brown et al., 2001; Darnell et al., 2001; Darnell et al., 2011; Santoro et al., 2012), among other functions (Contractor et al., 2015).”
From Unifying Views of Autism Spectrum Disorders: A Consideration of Autoregulatory Feedback Loops (2016) · cites this paper as Brown et al., 2001
In the citing paper
“FMRP regulates stability, subcellular transport, and translation of neuronal mRNAs encoding for proteins involved the in synaptic structure and function (58,(65)(66)(67).”
From Fragile X syndrome: causes, diagnosis, mechanisms, and therapeutics (2012) · cites this paper as [65]
In the citing paper
“the discovery that FMRP is involved in regulating the glucocorticoid receptor in the hippocampus (Brown et al., 2001).”
From Effects of intranasal oxytocin on social anxiety in males with fragile X syndrome (2012) · cites this paper as Brown et al., 2001
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cluster analysis and display of genome-wide expression patterns
Eisen, M. B., Spellman, P. T., Brown, P. O., & Botstein, D. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.25.14863 · Cited by 16,184
Verkerk, A. J. M. H., Pieretti, M., Sutcliffe, J. S., Fu, Y.-H., Kuhl, D. P. A., Pizzuti, A., Reiner, O., Richards, S., Victoria, M. F., Zhang, F., et al. (1991) · Cell · doi:10.1016/0092-8674(91)90397-h · Cited by 3,481
Self-citationInstability of a 550-Base Pair DNA Segment and Abnormal Methylation in Fragile X Syndrome
Oberlé, I., Rousseau, F., Heitz, D., Kretz, C., Devys, D., Hanauer, A., Boué, J., Bertheas, M. F., & Mandel, J. L. (1991) · Science · doi:10.1126/science.252.5009.1097 · Cited by 1,499
PSD-95 Involvement in Maturation of Excitatory Synapses
El-Husseini, A. E.-D., Schnell, E., Chetkovich, D. M., Nicoll, R. A., & Bredt, D. S. (2000) · Science · doi:10.1126/science.290.5495.1364 · Cited by 1,303
Fmr1 knockout mice: A model to study fragile X mental retardation
THEDUTCHBELGIANFRAGILEXCONSOR (1994) · Cell · doi:10.1016/0092-8674(94)90569-x · Cited by 1,108
Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function
Darnell, J. C., Jensen, K. B., Jin, P., Brown, V., Warren, S. T., & Darnell, R. B. (2001) · Cell · doi:10.1016/s0092-8674(01)00566-9 · Cited by 952
Self-citationFMR1 Protein: Conserved RNP Family Domains and Selective RNA Binding
Ashley, C. T., Wilkinson, K. D., Reines, D., & Warren, S. T. (1993) · Science · doi:10.1126/science.7692601 · Cited by 752
Self-citationDNA methylation represses FMR-1 transcription in fragile X syndrome
Sutcliffe, J. S., Nelson, D. L., Zhang, F., Pieretti, M., Caskey, C. T., Saxe, D., & Warren, S. T. (1992) · Human Molecular Genetics · doi:10.1093/hmg/1.6.397 · Cited by 680
Self-citationWeiler, I. J., Irwin, S. A., Klintsova, A. Y., Spencer, C. M., Brazelton, A. D., Miyashiro, K., Comery, T. A., Patel, B., Eberwine, J., & Greenough, W. T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.10.5395 · Cited by 644
The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein.
Siomi, H., Siomi, M. C., Nussbaum, R. L., & Dreyfuss, G. (1993) · Cell · doi:10.1016/0092-8674(93)90420-u · Cited by 641
A point mutation in the FMR-1 gene associated with fragile X mental retardation
Boulle, K. D., Verkerk, A. J. M. H., Reyniers, E., Vits, L., Hendrickx, J., Roy, B. V., Bos, F. V. D., de Graaff, E., Oostra, B. A., & Willems, P. J. (1993) · Nature Genetics · doi:10.1038/ng0193-31 · Cited by 624
Query, C. C., Bentley, R. C., & Keene, J. D. (1989) · Cell · doi:10.1016/0092-8674(89)90175-x · Cited by 580
Self-citationEvidence that fragile X mental retardation protein is a negative regulator of translation
Laggerbauer, B. (2001) · Human Molecular Genetics · doi:10.1093/hmg/10.4.329 · Cited by 571
Abnormal Development of Dendritic Spines in FMR1 Knock-Out Mice
Nimchinsky, E. A., Oberlander, A. M., & Svoboda, K. (2001) · The Journal of Neuroscience · doi:10.1523/jneurosci.21-14-05139.2001 · Cited by 560
Feng, Y., Gutekunst, C.-A., Eberhart, D. E., Yi, H., Warren, S. T., & Hersch, S. M. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-05-01539.1997 · Cited by 532
Self-citationAnalysis of neocortex in three males with the Fragile X syndrome
Hinton, V. J., Brown, W. T., Wisniewski, K., & Rudelli, R. D. (1991) · American Journal of Medical Genetics · doi:10.1002/ajmg.1320410306 · Cited by 527
Feng, Y., Absher, D., Eberhart, D. E., Brown, V., Malter, H. E., & Warren, S. T. (1997) · Molecular Cell · doi:10.1016/s1097-2765(00)80012-x · Cited by 511
Self-citationTurning on ARF: the Sec7 family of guanine-nucleotide-exchange factors
Jackson, C. L., & Casanova, J. E. (2000) · Trends in Cell Biology · doi:10.1016/s0962-8924(99)01699-2 · Cited by 480
The fragile X mental retardation protein inhibits translation via interacting with mRNA
Li, Z. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.11.2276 · Cited by 448
Siomi, H., Choi, M., Siomi, M. C., Nussbaum, R. L., & Dreyfuss, G. (1994) · Cell · doi:10.1016/0092-8674(94)90232-1 · Cited by 446
Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays
Tenenbaum, S. A., Carson, C. C., Lager, P. J., & Keene, J. D. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.26.14085 · Cited by 407
Self-citationEberhart, D. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.8.1083 · Cited by 390
Understanding the molecular basis of fragile X syndrome
Jin, P. (2000) · Human Molecular Genetics · doi:10.1093/hmg/9.6.901 · Cited by 369
Self-citationTakeuchi, M., Hata, Y., Hirao, K., Toyoda, A., Irie, M., & Takai, Y. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.18.11943 · Cited by 359
Schenck, A., Bardoni, B., Moro, A., Bagni, C., & Mandel, J.-L. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.151231598 · Cited by 356
The fragile X mental retardation syndrome protein interacts with novel homologs FXR1 and FXR2.
Zhang, Y., O'Connor, J. P., Siomi, M. C., Srinivasan, S., Dutra, A., Nussbaum, R. L., & Dreyfuss, G. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb00220.x · Cited by 337
Drosophila Unc-13 is essential for synaptic transmission
Aravamudan, B., Fergestad, T., Davis, W. S., Rodesch, C. K., & Broadie, K. S. (1999) · Nature Neuroscience · doi:10.1038/14764 · Cited by 335
Frey, D., Laux, T., Xu, L., Schneider, C., & Caroni, P. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.149.7.1443 · Cited by 289
Neuritin: A gene induced by neural activity and neurotrophins that promotes neuritogenesis
Naeve, G. S., Ramakrishnan, M., Kramer, R., Hevroni, D., Citri, Y., & Theill, L. E. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.6.2648 · Cited by 255
The fragile X mental retardation protein is associated with ribosomes
Khandjian, E. W., Corbin, F., Woerly, S., & Rousseau, F. (1996) · Nature Genetics · doi:10.1038/ng0196-91 · Cited by 251
Keene, J. D. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.111145598 · Cited by 231
Self-citationBrown, V., Small, K. M., Lakkis, L., Feng, Y., Gunter, C., Wilkinson, K. D., & Warren, S. T. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.25.15521 · Cited by 192
Self-citationCeman, S., Brown, V., & Warren, S. T. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.12.7925 · Cited by 181
Self-citationNeuronal abnormalities in microtubule-associated protein 1B mutant mice.
Edelmann, W., Zervas, M., Costello, P. S., Roback, L., Fischer, I., Hammarback, J. A., Cowan, N. J., Davies, P., Wainer, B. H., & Kucherlapati, R. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.3.1270 · Cited by 149
FMRP Involvement in Formation of Synapses among Cultured Hippocampal Neurons
Braun, K. A. (2000) · Cerebral Cortex · doi:10.1093/cercor/10.10.1045 · Cited by 149
Intrinsic neuronal determinants that promotes axonal sprouting and elongation
Caroni, P. (1997) · BioEssays · doi:10.1002/bies.950190906 · Cited by 145
FMRP is associated to the ribosomes via RNA
Tamanini, F. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.6.809 · Cited by 143
Bardoni, B., Schenck, A., & Louis Mandel, J. (1999) · Human Molecular Genetics · doi:10.1093/hmg/8.13.2557 · Cited by 136
Augustin, I., Korte, S., Rickmann, M., Kretzschmar, H. A., Südhof, T. C., Herms, J. W., & Brose, N. (2001) · Journal of Neuroscience · doi:10.1523/jneurosci.21-01-00010.2001 · Cited by 125
DAP‐1, a novel protein that interacts with the guanylate kinase‐like domains of hDLG and PSD‐95
Satoh, K., Yanai, H., Senda, T., Kohu, K., Nakamura, T., Okumura, N., Matsumine, A., Kobayashi, S., Toyoshima, K., & Akiyama, T. (1997) · Genes to Cells · doi:10.1046/j.1365-2443.1997.1310329.x · Cited by 121
Controlling small guanine–nucleotide-exchange factor function through cytoplasmic RNA intramers
Mayer, G., Blind, M., Nagel, W., Böhm, T., Knorr, T., Jackson, C. L., Kolanus, W., & Famulok, M. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.091100698 · Cited by 94
RNAs That Interact with the Fragile X Syndrome RNA Binding Protein FMRP
Sung, Y. J., Conti, J., Currie, J. R., Brown, W. T., & Denman, R. B. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.3405 · Cited by 82
Identification of Mouse YB1/p50 as a Component of the FMRP-Associated mRNP Particle
Ceman, S., Nelson, R., & Warren, S. T. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.4035 · Cited by 81
Self-citationPaschen, W., Blackstone, C. D., Huganir, R. L., & Ross, C. A. (1994) · Genomics · doi:10.1006/geno.1994.1198 · Cited by 51
Neeb, A., Koch, H., Schürmann, A., & Brose, N. (1999) · European Journal of Cell Biology · doi:10.1016/s0171-9335(99)80018-1 · Cited by 36
Ishizuka, T., Saisu, H., Odani, S., Kumanishi, T., & Abe, T. (1999) · Neuroscience · doi:10.1016/s0306-4522(98)00223-1 · Cited by 28
Development and Characterization of Antibodies that Immunoprecipitate the FMR1 Protein
Ceman, S., Zhang, F., Johnson, T., et al. (2003) · Neurogenetics · doi:10.1385/1-59259-330-5:345 · Cited by 11
Self-citation
Research works printed but not linked: 1
- 1994, Fmr1 knockout mice, Cell, 78, 23
Cited by
Sorted by citations
Mechanisms of Alternative Pre-Messenger RNA Splicing
Black, D. L. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161720 · Cited by 2,704
FMRP Stalls Ribosomal Translocation on mRNAs Linked to Synaptic Function and Autism
Darnell, J. C., Van Driesche, S. J., Zhang, C., Hung, K. Y. S., Mele, A., Fraser, C. E., Stone, E. F., Chen, C., Fak, J. J., Chi, S. W., et al. (2011) · Cell · doi:10.1016/j.cell.2011.06.013 · Cited by 2,072
Self-citationAdvances in autism genetics: on the threshold of a new neurobiology
Abrahams, B. S., & Geschwind, D. H. (2008) · Nature Reviews Genetics · doi:10.1038/nrg2346 · Cited by 1,866
The mGluR theory of fragile X mental retardation
Bear, M. F., Huber, K. M., & Warren, S. T. (2004) · Trends in Neurosciences · doi:10.1016/j.tins.2004.04.009 · Cited by 1,580
Self-citationAltered synaptic plasticity in a mouse model of fragile X mental retardation
Huber, K. M., Gallagher, S. M., Warren, S. T., & Bear, M. F. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.122205699 · Cited by 1,349
Self-citation
Metadata
- Authors
- Brown, V., Jin, P., Ceman, S., Darnell, J. C., O'Donnell, W. T., Tenenbaum, S. A., Jin, X., Feng, Y., Wilkinson, K. D., Keene, J. D., Darnell, R. B., & Warren, S. T.
- Affiliations
- Howard Hughes Medical Institute (US)
- Rockefeller University (US)
- Duke Medical Center (US)
- Emory University (US)
- Corresponding author
- Warren, Stephen T.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2001-11
- Volume
- 107
- Issue
- 4
- Pages
- 477-487
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2098756736
- Cited by
- 1,109
- References
- 47
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 20 · 2023: 28 · 2022: 27 · 2021: 33 · 2020: 36 · 2019: 41 · 2018: 39 · 2017: 39 · 2016: 43 · 2015: 39 · 2014: 56 · 2013: 57 · 2012: 51
- FWCI
- 33.21
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (T32GM008490, T32GM008169)
- National Institute of Neurological Disorders and Stroke (R01 NS34389, R01NS034389)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R37 HD20521, R01 HD40647, P01 HD35576, R37HD020521, R01HD040647, P01HD035576)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- fragile X syndrome, FMRP, G-quartet, RNA-binding proteins, mouse brain, candidate genes
- MeSH terms
- Amino Acid Sequence, Animals, Brain Chemistry, Centrifugation, Density Gradient, Disease Models, Animal, Fragile X Syndrome, Humans, Ligands, Mice, Inbred C57BL, Models, Genetic, Molecular Sequence Data, Nerve Tissue Proteins, Precipitin Tests, Protein Binding, Regulatory Sequences, Nucleic Acid, Ribosomes, RNA, Messenger, Protein Biosynthesis, Polymerase Chain Reaction, Sequence Alignment, RNA-Binding Proteins, Sequence Homology, Amino Acid, Mice, Knockout, Oligonucleotide Array Sequence Analysis, Macromolecular Substances, Mice, Fragile X Messenger Ribonucleoprotein 1, Fragile X Mental Retardation Protein
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
