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RETRACTED: Engineered whole organs and complex tissues
Abstract
End-stage organ failure is a key challenge for the medical community because of the ageing population and the severe shortage of suitable donor organs available. Equally, injuries to or congenital absence of complex tissues such as the trachea, oesophagus, or skeletal muscle have few therapeutic options. A new approach to treatment involves the use of three-dimensional biological scaffolds made of allogeneic or xenogeneic extracellular matrix derived from non-autologous sources. These scaffolds can act as an inductive template for functional tissue and organ reconstruction after recellularisation with autologous stem cells or differentiated cells. Such an approach has been used successfully for the repair and reconstruction of several complex tissues such as trachea, oesophagus, and skeletal muscle in animal models and human beings, and, guided by appropriate scientific and ethical oversight, could serve as a platform for the engineering of whole organs and other tissues.
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“Collagen- or fibrin-based products are the most established ECMs being used clinically to guide regeneration of different tissues, including skin, heart valves, trachea, muscle, and tendon (71, 139).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [139]
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Cites
2 retracted
RETRACTED: Clinical transplantation of a tissue-engineered airway
Macchiarini, P., Jungebluth, P., Go, T., Asnaghi, M. A., Rees, L., Cogan, T. A., Dodson, A., Martorell, J., Bellini, S., Parnigotto, P. P., et al. (2008) · The Lancet · doi:10.1016/s0140-6736(08)61598-6 · Cited by 1,431
Self-citationRetractedJungebluth, P., Alici, E., Baiguera, S., Blomberg, P., Bozóky, B., Crowley, C., Einarsson, Ó., Guðbjartsson, T., Guyader, S. L., Henriksson, G., et al. (2011) · The Lancet · doi:10.1016/s0140-6736(11)61715-7 · Cited by 424
Self-citationRetractedTakahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Endostatin: an endogenous inhibitor of angiogenesis and tumor growth
O'Reilly, M. S., Boehm, T., Shing, Y., Fukai, N., Vasios, G., Lane, W. S., Flynn, E., Birkhead, J. R., Olsen, B. R., & Folkman, J. (1997) · Cell · doi:10.1016/s0092-8674(00)81848-6 · Cited by 4,486
Reconstituting organ-level lung functions on a chip
Huh, D., Matthews, B. D., Mammoto, A., Montoya-Zavala, M., Hsin, H. Y., & Ingber, D. E. (2010) · Science · doi:10.1126/science.1188302 · Cited by 4,250
An overview of tissue and whole organ decellularization processes
Crapo, P. M., Gilbert, T. W., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.057 · Cited by 3,591
Self-citationPerfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Ott, H. C., Matthiesen, T. S., Goh, S.-K., Black, L. D., Kren, S. M., Netoff, T. I., & Taylor, D. A. (2008) · Nature Medicine · doi:10.1038/nm1684 · Cited by 2,650
Self-organizing optic-cup morphogenesis in three-dimensional culture
Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K., Adachi, T., & Sasai, Y. (2011) · Nature · doi:10.1038/nature09941 · Cited by 2,001
Tissue-engineered autologous bladders for patients needing cystoplasty
Atala, A., Bauer, S. B., Söker, S., Yoo, J. J., & Retik, A. B. (2006) · The Lancet · doi:10.1016/s0140-6736(06)68438-9 · Cited by 1,857
Murry, C. E., & Keller, G. M. (2008) · Cell · doi:10.1016/j.cell.2008.02.008 · Cited by 1,760
Research works printed but not linked: 2
- 2008, Deaths from chronic obstructive pulmonary disease—United States, 2000–2005, MMWR Morb Mortal Wkly Rep, 57, 1229
- Ott, 2005, A novel population of adult derived cardiac progenitor cells is capable of functional myocardial repair, Circulation, 112, II
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Injectable hydrogels for cartilage and bone tissue engineering
Liu, M., Zeng, X., Ma, C., Yi, H., Ali, Z., Mou, X., Li, S., Deng, Y., & He, N. (2017) · Bone Research · doi:10.1038/boneres.2017.14 · Cited by 1,236
The extracellular matrix: tools and insights for the "omics" era
Naba, A., Clauser, K. R., Ding, H., Whittaker, C. A., Carr, S. A., & Hynes, R. O. (2016) · Matrix Biology · doi:10.1016/j.matbio.2015.06.003 · Cited by 1,037
“Tissue engineers have exploited, to varying degrees, aspects of ECM biology, in particular its biomechanical properties (not discussed here), to design novel scaffolds to support tissue regeneration [92].”
Advancing biomaterials of human origin for tissue engineering
Chen, F.-M., & Liu, X. (2016) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2015.02.004 · Cited by 1,158
Role of the extracellular matrix in regulating stem cell fate
Watt, F. M., & Huck, W. T. S. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3620 · Cited by 861
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Liaw, C., & Güvendiren, M. (2017) · Biofabrication · doi:10.1088/1758-5090/aa7279 · Cited by 635
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Benders, K. E. M., van Weeren, P. R., Badylak, S. F., Saris, D. B. F., Dhert, W. J. A., & Malda, J. (2013) · Trends in biotechnology · doi:10.1016/j.tibtech.2012.12.004 · Cited by 539
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Fan, X., Zhang, Y., Li, X., & Fu, Q. (2020) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03454-6 · Cited by 551
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Xuan, K., Li, B., Guo, H., Sun, W., Kou, X., He, X., Zhang, Y. J., Sun, J., Liu, A., Liao, L., et al. (2018) · Science Translational Medicine · doi:10.1126/scitranslmed.aaf3227 · Cited by 500
Cardiopoietic Stem Cell Therapy in Heart Failure
Bartúnek, J., Behfar, A., Dolatabadi, D., Vanderheyden, M., Ostojić, M., Dens, J., Nakadi, B. E., Banovic, M. D., Beleslin, B., Vrolix, M., et al. (2013) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2013.02.071 · Cited by 504
A hydrogel derived from decellularized dermal extracellular matrix
Wolf, M. T., Daly, K. A., Brennan-Pierce, E. P., Johnson, S. A., Carruthers, C. A., D'Amore, A., Nagarkar, S. P., Velankar, S. S., & Badylak, S. F. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.06.051 · Cited by 482
“Recent studies suggest that some tissues respond more favorably to an ECM scaffold derived from homologous tissue [22,36].”
Self-citationChallenges With the Development of Biomaterials for Sustainable Tissue Engineering
Williams, D. F. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00127 · Cited by 306
“This resulted in the death of several patients and the retraction of some, apparently pivotal, studies and reviews (Jungebluth et al., 2011; Badylak et al., 2012).”
BackgroundCell therapy for cardiac repair—lessons from clinical trials
Behfar, A., Crespo‐Diaz, R. J., Terzic, A., & Gersh, B. J. (2014) · Nature Reviews Cardiology · doi:10.1038/nrcardio.2014.9 · Cited by 298
Advancing application of mesenchymal stem cell-based bone tissue regeneration
Shang, F., Yu, Y., Liu, S., Ming, L., Zhang, Y., Zhou, Z., Zhao, J., & Jin, Y. J. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.08.014 · Cited by 293
Parmaksiz, M., Dogan, A., Odabas, S., Elçin, A. E., & Elçin, Y. M. (2016) · Biomedical Materials · doi:10.1088/1748-6041/11/2/022003 · Cited by 268
Perfusion decellularization of human and porcine lungs: Bringing the matrix to clinical scale
Gilpin, S. E., Guyette, J. P., Gonzalez, G., Ren, X., Asara, J. M., Mathisen, D. J., Vacanti, J. P., & Ott, H. C. (2014) · The Journal of Heart and Lung Transplantation · doi:10.1016/j.healun.2013.10.030 · Cited by 263
Rana, D., Zreiqat, H., Benkirane-Jessel, N., Ramakrishna, S., & Ramalingam, M. (2017) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2061 · Cited by 243
“The methods involved in decellularization are classified as physical, chemical, biological/enzymatic or a combination of these approaches (Gilbert, 2012; Badylak et al., 2012), which rely on a common basic principle of cell membrane disruption for leaching out all the cellular contents.”
MethodDesigning Regenerative Biomaterial Therapies for the Clinic
Pashuck, E. T., & Stevens, M. M. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3002717 · Cited by 244
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Wolf, M. T., Dearth, C. L., Ranallo, C. A., LoPresti, S. T., Carey, L. E., Daly, K. A., Brown, B. N., & Badylak, S. F. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.04.115 · Cited by 236
“The ECM is a complex microenvironment comprised of collagen types, glycosaminoglycans, growth factors, and proteoglycans that are maintained during the cell removal process, and supports site appropriate tissue remodeling rather than scar formation [11,12].”
Self-citationHuman iPS cell–derived alveolar epithelium repopulates lung extracellular matrix
Ghaedi, M., Calle, E. A., Mendez, J. J., Gard, A. L., Balestrini, J. L., Booth, A., Bove, P. F., Gui, L., White, E. S., & Niklason, L. E. (2013) · Journal of Clinical Investigation · doi:10.1172/jci68793 · Cited by 234
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Wolf, M. T., Dearth, C. L., Sonnenberg, S. B., Loboa, E., & Badylak, S. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.08.011 · Cited by 214
“2F) have been created from ECM materials and have been well characterized [20,44].”
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Ng, W. L., Lee, J. M., Yeong, W. Y., & Naing, M. W. (2017) · Biomaterials Science · doi:10.1039/c6bm00861e · Cited by 231
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Faulk, D. M., Carruthers, C. A., Warner, H. J., Kramer, C. R., Reing, J. E., Zhang, L., D’Amore, A., & Badylak, S. F. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.09.006 · Cited by 220
Self-citationBaiguera, S., Gaudio, C. D., Lucatelli, E., Kuevda, E. V., Boieri, M., Mazzanti, B., Bianco, A., & Macchiarini, P. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.10.060 · Cited by 211
Self-citationQadura, M., Terenzi, D. C., Verma, S., Al-Omran, M. A., & Hess, D. A. (2017) · Stem Cells · doi:10.1002/stem.2751 · Cited by 200
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Orlando, G., Booth, C., Wang, Z., Totonelli, G., Ross, C. L., Moran, E., Salvatori, M., Maghsoudlou, P., Turmaine, M., Delario, G., et al. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.04.033 · Cited by 194
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Self-citationHan, T. T. Y., Toutounji, S., Amsden, B. G., & Flynn, L. E. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.053 · Cited by 150
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BackgroundUterine stem cells: from basic research to advanced cell therapies
Santamaría, X., Mas, A., Cervelló, I., Taylor, H. S., & Simón, C. (2018) · Human Reproduction Update · doi:10.1093/humupd/dmy028 · Cited by 143
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BackgroundWagner, D. E., Bonenfant, N. R., Sokocevic, D., DeSarno, M., Borg, Z. D., Parsons, C. S., Brooks, E. M., Platz, J. J., Khalpey, Z. I., Hoganson, D. M., et al. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.078 · Cited by 141
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Li, Q., Uygun, B. E., Geerts, S., Özer, S., Scalf, M., Gilpin, S. E., Ott, H. C., Yarmush, M. L., Smith, L. M., Welham, N. V., et al. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.10.011 · Cited by 134
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BackgroundSelf-citationPartington, L., Mordan, N. J., Mason, C., Knowles, J. C., Kim, H.-W., Lowdell, M. W., Birchall, M., & Wall, I. B. (2012) · Acta Biomaterialia · doi:10.1016/j.actbio.2012.10.004 · Cited by 127
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BackgroundElliott, M. J., Butler, C. R., Varanou, A., Partington, L., Carvalho, C., Samuel, E. R., Crowley, C., Lange, P., Hamilton, N., Hynds, R. E., et al. (2017) · Stem Cells Translational Medicine · doi:10.1002/sctm.16-0443 · Cited by 120
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Faissner, A., & Reinhard, J. (2015) · Glia · doi:10.1002/glia.22839 · Cited by 116
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Song, J. S., Takimoto, K., Jeon, M., Vadakekalam, J., Ruparel, N. B., & Diogenes, A. R. (2017) · Journal of Dental Research · doi:10.1177/0022034517693606 · Cited by 117
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BackgroundXu, Y., Li, D., Yin, Z., He, A., Lin, M., Jiang, G., Song, X., Hu, X., Liu, Y., Wang, J., et al. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.05.010 · Cited by 116
Wagner, D. E., Bonvillain, R. W., Jensen, T., Girard, E. D., Bunnell, B. A., Finck, C. M., Hoffman, A. M., & Weiss, D. J. (2013) · Respirology · doi:10.1111/resp.12102 · Cited by 120
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Price, A., Godin, L. M., Domek, A., Cotter, T., D’Cunha, J., Taylor, D. A., & Panoskaltsis‐Mortari, A. (2014) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2013.0756 · Cited by 105
Utomo, L., Pleumeekers, M. M., Nimeskern, L., Nürnberger, S., Stok, K. S., Hildner, F., & van Osch, G. J. V. M. (2015) · Biomedical Materials · doi:10.1088/1748-6041/10/1/015010 · Cited by 100
Sokocevic, D., Bonenfant, N. R., Wagner, D. E., Borg, Z. D., Lathrop, M. J., Lam, Y. W., Deng, B., DeSarno, M., Ashikaga, T., Loi, R., et al. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.01.028 · Cited by 98
Self-citationNadal‐Ginard, B., Ellison, G. M., & Torella, D. (2014) · Stem Cell Research · doi:10.1016/j.scr.2014.04.008 · Cited by 105
The effects of storage and sterilization on de-cellularized and re-cellularized whole lung
Bonenfant, N. R., Sokocevic, D., Wagner, D. E., Borg, Z. D., Lathrop, M. J., Lam, Y. W., Deng, B., DeSarno, M., Ashikaga, T., Loi, R., et al. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.01.031 · Cited by 97
“Whether this will subsequently affect re-cellularization of scaffolds and therefore the generation of functional tissue suitable for transplantation, remains unresolved [4,5].”
BackgroundSelf-citationHeterogeneity of Scaffold Biomaterials in Tissue Engineering
Edgar, L., McNamara, K., Wong, T., Tamburrini, R., Katari, R., & Orlando, G. (2016) · Materials · doi:10.3390/ma9050332 · Cited by 97
Alveolar epithelial differentiation of human induced pluripotent stem cells in a rotating bioreactor
Ghaedi, M., Mendez, J. J., Bove, P. F., Sivarapatna, A., Raredon, M. S. B., & Niklason, L. E. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.10.018 · Cited by 93
“Given that iPS cells can be derived from the patient to be treated, they could provide a cell source that is genetically identical to the patient, allowing tissue that is generated from these cells to avoid immune rejection [9,13].”
Chae, S., Sun, Y., Choi, Y., Ha, D., Jeon, I., & Cho, D. (2020) · Biofabrication · doi:10.1088/1758-5090/abd159 · Cited by 94
“We believed that this phenomenon may occur from a concurrent biomimetic approach and the co-existence of tissue-specific matrix components, growth factors, and/or signaling molecules [55].”
Rosselló, R. A., Chen, C., Dai, R., Howard, J. T., Hochgeschwender, U., & Jarvis, E. D. (2013) · eLife · doi:10.7554/elife.00036 · Cited by 91
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BackgroundTuomi, J., Paloheimo, K., Vehviläinen, J., Björkstrand, R., Salmi, M., Huotilainen, E., Kontio, R. K., Rouse, S., Gibson, I., & Mäkitie, A. A. (2014) · Surgical Innovation · doi:10.1177/1553350614524838 · Cited by 89
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Trachea transplantation: from laboratory to patient
Crowley, C., Birchall, M., & Seifalian, A. M. (2014) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1847 · Cited by 87
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BackgroundMethodZvarova, B., Uhl, F. E., Uriarte, J. J., Borg, Z. D., Coffey, A. L., Bonenfant, N. R., Weiss, D. J., & Wagner, D. E. (2016) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2015.0439 · Cited by 92
Self-citationAirway tissue engineering: an update
Fishman, J. M., Wiles, K., Lowdell, M. W., Coppi, P. D., Elliott, M. J., Atala, A., & Birchall, M. (2014) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2014.938631 · Cited by 85
Cai, R., Nakamoto, T., Kawazoe, N., & Chen, G. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.02.033 · Cited by 84
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BackgroundPirosa, A., Gottardi, R., Alexander, P. G., & Tuan, R. S. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-0847-8 · Cited by 81
Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review
Vrana, N. E., Lavall�e, P., Dokmeci, M. R., Dehghani, F., Ghaemmaghami, A. M., & Khademhosseini, A. (2013) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2012.0603 · Cited by 80
He, M., Callanan, A., Lagaras, K., Steele, J. A. M., & Stevens, M. M. (2016) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33668 · Cited by 81
Stem Cells and Cell Therapies in Lung Biology and Diseases: Conference Report
Weiss, D. J., Bates, J. H. T., Gilbert, T. W., Liles, W. C., Lutzko, C., Rajagopal, J., & Prockop, D. J. (2013) · Annals of the American Thoracic Society · doi:10.1513/annalsats.201304-089aw · Cited by 79
Self-citationDua, K. S., Hogan, W. J., Aadam, A. A., & Gasparri, M. (2016) · The Lancet · doi:10.1016/s0140-6736(15)01036-3 · Cited by 79
Stem Cells and Tissue Engineering
Strong, A. L., Neumeister, M. W., & Lévi, B. (2017) · Clinics in Plastic Surgery · doi:10.1016/j.cps.2017.02.020 · Cited by 85
Decellularized musculofascial extracellular matrix for tissue engineering
Wang, L., Johnson, J. A., Chang, D. W., & Zhang, Q. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2012.12.048 · Cited by 78
“Decellularization, a technique to remove cell components and maintain the ECM of tissues, provides a means of studying the native ECM [1,7].”
Native extracellular matrix: a new scaffolding platform for repair of damaged muscle
Teodori, L., Costa, A., Marzio, R., Perniconi, B., Coletti, D., Adamo, S., Gupta, B., & Tarnok, A. (2014) · Frontiers in Physiology · doi:10.3389/fphys.2014.00218 · Cited by 84
“Appropriate tissue decellularization preserves not only the ECM integrity, bioactivity and spatial structure, but also the vascular, lymphatic and nervous network (Badylak et al., 2012).”
BackgroundEpithelial Cell Differentiation of Human Mesenchymal Stromal Cells in Decellularized Lung Scaffolds
Mendez, J. J., Ghaedi, M., Steinbacher, D. M., & Niklason, L. E. (2014) · Tissue Engineering Part A · doi:10.1089/ten.tea.2013.0647 · Cited by 76
Microenvironment-targeted strategy steers advanced bone regeneration
Hao, S., Wang, M., Yin, Z., Jing, Y., Bai, L., & Su, J. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100741 · Cited by 80
Napierala, H., Hillebrandt, K. H., Haep, N., Tang, P., Tintemann, M., Gassner, J. M. G. V., Nösser, M., Everwien, H., Seiffert, N., Kluge, M., et al. (2017) · Scientific Reports · doi:10.1038/srep41777 · Cited by 77
Wu, Y., Wang, J., Shi, Y., Pu, H., Leak, R. K., Liou, A. K. F., Badylak, S. F., Liu, Z., Zhang, J., Chen, J., et al. (2017) · Cell Transplantation · doi:10.1177/0963689717714090 · Cited by 74
“ECM consists of various tissue-specific structural and functional molecules that affect resident cells, including collagen, glycosaminoglycans, proteoglycans, and growth factors.(24) Viable cells release the appropriate factors into the ECM that facilitate their communication with neighboring cells and continue to sup…”
Self-citationBone Graft Prefabrication Following the In Vivo Bioreactor Principle
Huang, R., Kobayashi, E., Liu, K., & Li, Q. (2016) · EBioMedicine · doi:10.1016/j.ebiom.2016.09.016 · Cited by 74
“The decellularized organ scaffold then serves as an inductive biological template around which the recipient rebuilds functional tissue through the recruitment of endogenous cells following the IVB principle (Badylak et al., 2012).”
Biofabrication of valentine-shaped heart with a composite hydrogel and sacrificial material
Zou, Q., Grottkau, B. E., He, Z., Shu, L., Yang, L., Ma, M., & Ye, C. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.110205 · Cited by 74
Esophageal tissue engineering: A new approach for esophageal replacement
Totonelli, G. (2012) · World Journal of Gastroenterology · doi:10.3748/wjg.v18.i47.6900 · Cited by 73
Maghsoudlou, P., Georgiades, F., Tyraskis, A., Totonelli, G., Loukogeorgakis, S., Orlando, G., Shangaris, P., Lange, P., Delalande, J., Burns, A. J., et al. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.05.015 · Cited by 69
Advances in Tracheal Reconstruction
Haykal, S., Salna, M. P., Waddell, T. K., & Hofer, S. O. P. (2014) · Plastic & Reconstructive Surgery Global Open · doi:10.1097/gox.0000000000000097 · Cited by 70
On the Genealogy of Tissue Engineering and Regenerative Medicine
Kaul, H., & Ventikos, Y. (2014) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2014.0285 · Cited by 72
Experimental Evaluation of Kidney Regeneration by Organ Scaffold Recellularization
Remuzzi, A., Figliuzzi, M., Bonandrini, B., Silvani, S., Azzollini, N., Nossa, R., Benigni, A., & Remuzzi, G. (2017) · Scientific Reports · doi:10.1038/srep43502 · Cited by 68
Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel
Xu, Y., & Wang, X. (2015) · Biotechnology and Bioengineering · doi:10.1002/bit.25579 · Cited by 65
“Organ donor shortage has been a serious social problem for many years due to heavy traffic accidents and organ failures (Badylak et al., 2012; Rahaman and Mao, 2005; Verhulsela et al., 2014; Wang et al., 2007,2009; Wang, 2012).”
Struecker, B., Butter, A., Hillebrandt, K., Polenz, D., Reutzel-Selke, A., Tang, P., Lippert, S., Leder, A., Rohn, S., Geisel, D., et al. (2017) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1948 · Cited by 65
Inhomogeneity of local stiffness in the extracellular matrix scaffold of fibrotic mouse lungs
Melo, E., Cárdenes, N., Garreta, E., Luque, T., Rojas, M. M., Navajas, D., & Farré, R. (2014) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2014.05.019 · Cited by 62
Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats
Sjöqvist, S., Jungebluth, P., Lim, M. L., Haag, J. C., Gustafsson, Y., Lemon, G., Baiguera, S., Burguillos, M. Á., Gaudio, C. D., Rodríguez, A. B., et al. (2014) · Nature Communications · doi:10.1038/ncomms4562 · Cited by 63
Self-citationRetractedCrowley, C., Klanrit, P., Butler, C. R., Varanou, A., Platé, M., Hynds, R. E., Chambers, R. C., Seifalian, A. M., Birchall, M., & Janes, S. M. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.01.005 · Cited by 63
Weiss, D. J. (2013) · Annals of the American Thoracic Society · doi:10.1513/annalsats.201304-090aw · Cited by 62
Self-citationSun, W. Q., Xu, H. M., Sandor, M., & Lombardi, J. (2013) · Journal of Tissue Engineering · doi:10.1177/2041731413505305 · Cited by 62
Experimental approaches to vascularisation within tissue engineering constructs
Sarker, M. D., Chen, D., & Schreyer, D. J. (2015) · Journal of Biomaterials Science Polymer Edition · doi:10.1080/09205063.2015.1059018 · Cited by 64
“Moreover, preserving the native properties of ECM, for example, three-dimensional ultrastructure, surface topography, composition, bioactivity, and density of ligand distribution as well as internal network, such as nervous, vascular, and lymphatic networks, presents another technical challenge.[76] One of the importa…”
Effects of the Decellularization Method on the Local Stiffness of Acellular Lungs
Melo, E., Garreta, E., Luque, T., Cortiella, J., Nichols, J. E., Navajas, D., & Farré, R. (2013) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2013.0325 · Cited by 60
The Pharmacology of Regenerative Medicine
Christ, G. J., Saul, J. M., Furth, M. E., & Andersson, K. (2013) · Pharmacological Reviews · doi:10.1124/pr.112.007393 · Cited by 63
Sterilization of Lung Matrices by Supercritical Carbon Dioxide
Balestrini, J. L., Liu, A., Gard, A. L., Huie, J. L., Blatt, K. M. S., Schwan, J., Zhao, L., Broekelmann, T. J., Mecham, R. P., Wilcox, E. C., et al. (2015) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2015.0449 · Cited by 67
“Two remaining challenges critical to the clinical success of a donor-compatible organ transplant are (i) the ability to suppress the growth of resident infectious bacteria and other microorganisms, and (ii) the ability to produce a scaffold that is stable over long-term storage.(8,9) The availability of sterile lung s…”
Novel detergent for whole organ tissue engineering
Kawasaki, T., Kirita, Y., Kami, D., Kitani, T., Ozaki, C., Itakura, Y., Toyoda, M., & Gojo, S. (2015) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35474 · Cited by 61
Metadata
- Authors
- Badylak, S. F., Weiss, D. J., Caplan, A., & Macchiarini, P.
- Affiliations
- University of Pittsburgh (US)
- University of Vermont (US)
- University of Pennsylvania (US)
- Corresponding author
- Macchiarini, Paolo
- Venue
- The Lancet
- Publisher
- Elsevier BV
- Published
- 2012-03
- Volume
- 379
- Issue
- 9819
- Pages
- 943-952
- ISSN
- 0099-5355 (ISSN-L)
- 0140-6736
- OpenAlex
- W2158646376
- Cited by
- 417
- References
- 92
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 2 · 2023: 8 · 2022: 5 · 2021: 11 · 2020: 14 · 2019: 19 · 2018: 27 · 2017: 52 · 2016: 51 · 2015: 57 · 2014: 65 · 2013: 78 · 2012: 23
- FWCI
- 72.82
- Type
- journal article
- Language
- en
- Status
- Retracted
- Update notices
- Open access
- closed
- Locations
- The Lancet (publishedVersion)
- PubMed (publishedVersion)
- Funders
- the Wallenberg Institute for Regenerative Medicine and Karolinska Institutet/SLL grants (Stockholm, Sweden) and grants from the US National Institutes of Health ((RC4HL106625))
- National Heart, Lung, and Blood Institute ((R21HL094611))
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- tissue engineering, extracellular matrix, stem cells, end-stage organ failure, organ shortage, retraction
- MeSH terms
- Extracellular Matrix, Humans, Bioreactors, Transplants, Tissue Engineering, Tissue Scaffolds
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