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Cited by 64Parasitic Diseases Research and TreatmentHelminth infection and controlResearch on Leishmaniasis Studies

Specific polymerase chain reaction for differential diagnosis of Dirofilaria immitis and Dipetalonema reconditum using primers derived from internal transcribed spacer region 2 (ITS2)

Mar, Ping-Her, Yang, I-Chen, Chang, Gan-Nan, Fei, Andrew Chang-Young·2002·Veterinary Parasitology
DOI 10.1016/s0304-4017(02)00032-8
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Abstract

Both Dirofilaria immiti and Dipetalonema reconditum may be found in blood of infected dogs but it is not easy to distinguish D. immitis from D. reconditum in morphology. We cloned and sequenced the contiguous internal transcribed spacer (ITS) region, ITS1-5.8S-ITS2, of these two different parasites and published on GenBank as AF217800 for D. immiti and AF217801 for D. reconditum in this study. We designed two pairs of specific primers derived from ITS2 being used for polymerase chain reaction (PCR). The amplicons of ITS2 from D. immiti and D. reconditum are 302 and 348bp, respectively. Moreover, the limitation for amplifying ITS2 gene using this PCR demonstrated that 1 x 10(-2) microfilaria of each species of parasite smashed or even with mixed samples could be detected and the PCR products were predicted as the same as that described above. Thus, D. immiti and D. reconditum could be differentially diagnosed by this specific PCR. Seventeen clinical cases were evaluated and all of them were correctly identified. In this study, ITS1-5.8S-ITS2 of D. immiti or D. reconditum were the first time sequenced and analyzed. No significant similarity of ITS1 and ITS2 between D. immiti and D. reconditum could be observed.

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  • The prevalence of Dirofilaria spp. can be over-estimated if other filarial species are present and misidentified.

    “Thus, the prevalence of Dirofilaria spp. can be over-estimated if other filarial species are present and misidentified [13, 14]”

    From Molecular characterization of Dirofilaria spp. circulating in Portugal (2017) · cites this paper as [14]

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Authors
Mar, P.-H., Yang, I.-C., Chang, G.-N., & Fei, A. C.-Y.
Affiliations
National Pingtung University of Science and Technology (TW)
Venue
Veterinary Parasitology
Publisher
Elsevier BV
Published
2002-06
Volume
106
Issue
3
Pages
243-252
ISSN
0304-4017 (ISSN-L)
DOI
10.1016/s0304-4017(02)00032-8
OpenAlex
W2007521738
Cited by
64
References
27
Citations by year
2026: 1 · 2025: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 5 · 2019: 2 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 4 · 2014: 4 · 2013: 4 · 2012: 4
FWCI
1.54
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Veterinary Parasitology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
Keywords
Dirofilaria immitis, Dipetalonema reconditum, canine heartworm disease, differential diagnosis, ITS2, internal transcribed spacer region, ribosomal DNA, microfilariae, molecular identification, dogs
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Diagnosis, Differential, Dipetalonema, Dipetalonema Infections, Dirofilaria immitis, Dirofilariasis, Dog Diseases, Dogs, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Alignment, DNA, Helminth, DNA, Ribosomal Spacer

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