Cited by 96Parasitic Diseases Research and TreatmentHelminth infection and controlResearch on Leishmaniasis Studies

A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris)

Casiraghi, Maurizio, Bazzocchi, Chiara, Mortarino, Michele, Ottina, Eleonora, Genchi, Claudio·2006·Veterinary Parasitology

Abstract

Accurate diagnosis of canine filariosis is essential for choosing correct therapeutic approach. Therefore, reliable methods for discriminating among the different filarial infections in dogs are needed. The authors report simple and highly specific molecular methods that identify the three most common filarial nematodes of European dogs: Dirofilaria immitis, D. repens and Acanthocheilonema (syn. Dipetalonema) reconditum, based on (1) PCR amplifications of mitochondrial DNA (12S rDNA and coxI) with general filarial primers followed by digestion with restriction enzymes that generates band polymorphisms clearly discriminating the three species and (2) PCR amplifications with species-specific primers to support the restriction analysis, in particular in the case of multiple infections.

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Crossref · 10.1016/j.vetpar.2006.06.006

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  • Recent molecular-based assays have been reported for the unequivocal identification of filarioids, irrespective of their life cycle stage.

    “Recent molecular-based assays have been reported for the unequivocal identification of filarioids, irrespective of their life cycle stage.”

    From Vector-borne helminths of dogs and humans in Europe (2013) · cites this paper as [126]

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Authors
Casiraghi, M., Bazzocchi, C., Mortarino, M., Ottina, E., & Genchi, C.
Affiliations
  • University of Milano-Bicocca (IT)
  • University of Milan (IT)
Corresponding author
Genchi, Claudio
Venue
Veterinary Parasitology
Publisher
Elsevier BV
Published
2006-11
Volume
141
Issue
3-4
Pages
368-372
ISSN
0304-4017 (ISSN-L)
OpenAlex
W2066943571
Cited by
96
References
13
Citations by year
2026: 2 · 2025: 3 · 2024: 4 · 2023: 2 · 2022: 15 · 2021: 1 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 5 · 2013: 5 · 2012: 5
FWCI
3.08
Type
journal article
Language
en
Open access
closed
Locations
  • Veterinary Parasitology (publishedVersion)
  • PubMed (publishedVersion)
  • BOA (University of Milano-Bicocca) (submittedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
Keywords
Dirofilaria immitis, Dirofilaria repens, Acanthocheilonema reconditum, filarial nematodes, DNA-based species identification, mitochondrial DNA, 12S rDNA, coxI, restriction fragment length polymorphism, species-specific primers, dogs
MeSH terms
Animals, Base Sequence, Diagnosis, Differential, Dipetalonema, Dipetalonema Infections, Dirofilaria, Dirofilaria immitis, Dirofilariasis, DNA, Mitochondrial, Dog Diseases, Dogs, Species Specificity, Polymerase Chain Reaction, DNA, Helminth

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