Characterization of Wnt gene expression in developing and postnatal hair follicles and identification of Wnt5a as a target of Sonic hedgehog in hair follicle morphogenesis
Open accessAbstract
Mutations in WNT effector genes perturb hair follicle morphogenesis, suggesting key roles for WNT proteins in this process. We show that expression of Wnts 10b and 10a is upregulated in placodes at the onset of follicle morphogenesis and in postnatal hair follicles beginning a new cycle of hair growth. The expression of additional Wnt genes is observed in follicles at later stages of differentiation. Among these, we find that Wnt5a is expressed in the developing dermal condensate of wild type but not Sonic hedgehog (Shh)-null embryos, indicating that Wnt5a is a target of SHH in hair follicle morphogenesis. These results identify candidates for several key follicular signals and suggest that WNT and SHH signaling pathways interact to regulate hair follicle morphogenesis.
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Expression levels of Apc, Axin1, Lef, Lrp5, and Wnt10b were significantly increased in hair follicloids.
“Specifically, the expression levels of genes involved in hair follicle development—Apc, Axin1, Lef, Lrp5, and Wnt10b (32, 35)—were significantly increased in hair follicloids (Fig. 5, A and B).”
From Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction (2022) · cites this paper as [35]
The Introduction links Lef1 to hair follicle development, and section 2.2 reports elevated Wnt10b expression in developing hair follicles. The citation is attached to the description of the genes as involved in follicle development, not to the hair-follicloid increase shown in the citing paper’s Fig. 5. Our copy does not establish that role for Apc, Axin1, or Lrp5. (LLM, conf=0.86)
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Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction
Kageyama, T., Shimizu, A., Anakama, R., Nakajima, R., Suzuki, K., Okubo, Y., & Fukuda, J. (2022) · Science Advances · doi:10.1126/sciadv.add4603 · Cited by 73 · see citing works
- Authors
- Reddy, S., Andl, T., Bagasra, A., Lu, M. M., Epstein, D. J., Morrisey, E. E., & Millar, S. E.
- Affiliations
- University of Pennsylvania (US)
- Corresponding author
- Millar, Sarah E.
- Venue
- Mechanisms of Development
- Publisher
- Elsevier BV
- Published
- 2001-09
- Volume
- 107
- Issue
- 1-2
- Pages
- 69-82
- ISSN
- 0925-4773 (ISSN-L)
- OpenAlex
- W2084385289
- Cited by
- 489
- References
- 5
- Citations by year
- 2026: 10 · 2025: 18 · 2024: 13 · 2023: 25 · 2022: 29 · 2021: 13 · 2020: 23 · 2019: 14 · 2018: 20 · 2017: 20 · 2016: 15 · 2015: 16 · 2014: 26 · 2013: 26 · 2012: 24
- FWCI
- 21.21
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Mechanisms of Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Dermatology Foundation
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Keywords
- Wnt signaling, Wnt5a, Sonic hedgehog, hair follicle morphogenesis, placodes, WNT10A, Wnt10b, hair cycle, gene expression
- MeSH terms
- Wnt-5a Protein, Animals, Embryonic Induction, Morphogenesis, Mutation, Nerve Tissue Proteins, Proto-Oncogene Proteins, Skin, Signal Transduction, Trans-Activators, Up-Regulation, In Situ Hybridization, Gene Expression Regulation, Developmental, Hair Follicle, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Wnt Proteins, Mice, Hedgehog Proteins
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