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Compartmentalization of β-secretase (Asp2) into low-buoyant density, noncaveolar lipid rafts

Riddell, David R., Christie, Gary, Hussain, Ishrut, Dingwall, Colin·2001·Current Biology
DOI 10.1016/s0960-9822(01)00394-3
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Abstract

Recent epidemiological studies show a reduced prevalence of Alzheimer's disease (AD) in patients treated with inhibitors of cholesterol biosynthesis. Moreover, the cholesterol-transport protein, apolipoprotein E4, and elevated cholesterol are important risk factors for AD. Additionally, in vitro and in vivo studies show that intracellular cholesterol levels can modulate the processing of amyloid precursor protein (APP) to beta-amyloid, the major constituent of senile plaques. Cholesterol plays a crucial role in maintaining lipid rafts in a functional state. Lipid rafts are cholesterol-enriched membrane microdomains implicated in signal transduction, protein trafficking, and proteolytic processing. Since APP, beta-amyloid, and the putative gamma-secretase, presenilin-1 (PS-1), have all been found in lipid rafts, we hypothesized that the recently identified beta-secretase, Asp2 (BACE1), might also be present in rafts. Here, we report that recombinant Asp2 expressed in three distinct cell lines is raft associated. Using both detergent and nondetergent methods, Asp2 protein and activity were found in a light membrane raft fraction that also contained other components of the amyloidogenic pathway. Immunoisolation of caveolin-containing vesicles indicated that Asp2 was present in a unique raft population distinct from caveolae. Finally, depletion of raft cholesterol abrogated association of Asp2 with the light membrane fraction. These observations are consistent with the raft local…

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Research works printed but not linked: 31

  1. Wolozin, 2000, Decreased prevalence of Alzheimer's disease associated with 3-hydroxy-3-methyglutaryl coenzyme A reductase inhibitors, Arch Neurol, 57, 1439, 10.1001/archneur.57.10.1439 · DOI
  2. Jick, 2000, Statins and the risk of dementia, Lancet, 356, 1627, 10.1016/S0140-6736(00)03155-X · DOI
  3. Kuo, 1998, Elevated low-density lipoprotein in Alzheimer's disease correlates with brain abeta 1-42 levels, Biochem Biophys Res Commun, 252, 711, 10.1006/bbrc.1998.9652 · DOI · Paper page
  4. Notkola, 1998, Serum total cholesterol, apolipoprotein E epsilon 4 allele, and Alzheimer's disease, Neuroepidemiology, 17, 14, 10.1159/000026149 · DOI
  5. Corder, 1993, Gene dose of apolipoprotein E type 4 allele and the risk of Alzheimer's disease in late onset families, Science, 261, 921, 10.1126/science.8346443 · DOI · Paper page
  6. Simons, 1998, Cholesterol depletion inhibits the generation of beta-amyloid in hippocampal neurons, Proc Natl Acad Sci USA, 95, 6460, 10.1073/pnas.95.11.6460 · DOI
  7. Frears, 1999, The role of cholesterol in the biosynthesis of beta-amyloid, Neuroreport, 10, 1699, 10.1097/00001756-199906030-00014 · DOI
  8. Austen, 2000, The use of seldi proteinchip arrays to monitor production of Alzheimer's beta-amyloid in transfected cells, J Pept Sci, 6, 459, 10.1002/1099-1387(200009)6:9<459::AID-PSC286>3.0.CO;2-B · DOI
  9. Refolo, 2000, Hypercholesterolemia accelerates the Alzheimer's amyloid pathology in a transgenic mouse model, Neurobiol Dis, 7, 321, 10.1006/nbdi.2000.0304 · DOI · Paper page
  10. Sparks, 1994, Induction of Alzheimer-like beta-amyloid immunoreactivity in the brains of rabbits with dietary cholesterol, Exp Neurol, 126, 88, 10.1006/exnr.1994.1044 · DOI · Paper page
  11. Sparks, 2000, Alterations of Alzheimer's disease in the cholesterol-fed rabbit, including vascular inflammation. Preliminary observations, Ann N Y Acad Sci, 903, 335, 10.1111/j.1749-6632.2000.tb06384.x · DOI
  12. Hooper, 2000, The role of proteolysis in Alzheimer's disease, Adv Exp Med Biol, 477, 379, 10.1007/0-306-46826-3_39 · DOI
  13. Howlett, 2000, In search of an enzyme, Trends Neurosci, 23, 565, 10.1016/S0166-2236(00)01647-7 · DOI
  14. De Strooper, 2000, Proteolytic processing and cell biological functions of the amyloid precursor protein, J Cell Sci, 113, 1857, 10.1242/jcs.113.11.1857 · DOI
  15. Simons, 2000, How cells handle cholesterol, Science, 290, 1721, 10.1126/science.290.5497.1721 · DOI
  16. Brown, 2000, Structure and function of sphingolipid- and cholesterol-rich membrane rafts, J Biol Chem, 275, 17221, 10.1074/jbc.R000005200 · DOI
  17. Simons, 2000, Lipid rafts and signal transduction, Nat Rev Mol Cell Biol, 1, 31, 10.1038/35036052 · DOI · Paper page
  18. Lee, 1998, A detergent-insoluble membrane compartment contains A beta in vivo, Nat Med, 4, 730, 10.1038/nm0698-730 · DOI
  19. Morishima-Kawashima, 1998, The presence of amyloid beta-protein in the detergent-insoluble membrane compartment of human neuroblastoma cells, Biochemistry, 37, 15247, 10.1021/bi981843u · DOI
  20. Parkin, 1999, Characterization of detergent-insoluble complexes containing the familial Alzheimer's disease-associated presenilins, J Neurochem, 72, 1534, 10.1046/j.1471-4159.1999.721534.x · DOI
  21. Fiedler, 1993, Glycosphingolipid-enriched, detergent-insoluble complexes in protein sorting in epithelial cells, Biochemistry, 32, 6365, 10.1021/bi00076a009 · DOI
  22. Roper, 2000, Retention of prominin in microvilli reveals distinct cholesterol-based lipid micro-domains in the apical plasma membrane, Nat Cell Biol, 2, 582, 10.1038/35023524 · DOI
  23. Waugh, 1999, Epidermal growth factor receptor activation is localized within low-buoyant density, non-caveolar membrane domains, Biochem J, 337, 591, 10.1042/0264-6021:3370591 · DOI
  24. Hussain, 1999, Identification of a novel aspartic protease (Asp 2) as beta-secretase, Mol Cell Neurosci, 14, 419, 10.1006/mcne.1999.0811 · DOI · Paper page
  25. Feng, 2001, Caveolin-1 associates with TRAF2 to form a complex that is recruited to TNF receptors, J Biol Chem, 276, 8341, 10.1074/jbc.M007116200 · DOI
  26. Capell, 2000, Maturation and pro-peptide cleavage of beta-secretase, J Biol Chem, 275, 30849, 10.1074/jbc.M003202200 · DOI · Paper page
  27. Hussain, 2000, ASP1 (BACE2) cleaves the amyloid precursor protein at the beta-secretase site, Mol Cell Neurosci, 16, 609, 10.1006/mcne.2000.0884 · DOI
  28. Ikezu, 1998, Caveolae, plasma membrane microdomains for alpha-secretase-mediated processing of the amyloid precursor protein, J Biol Chem, 273, 10485, 10.1074/jbc.273.17.10485 · DOI
  29. Keller, 1998, Cholesterol is required for surface transport of influenza virus hemagglutinin, J Cell Biol, 140, 1357, 10.1083/jcb.140.6.1357 · DOI
  30. Selkoe, 1994, Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease, Annu Rev Cell Biol, 10, 373, 10.1146/annurev.cb.10.110194.002105 · DOI
  31. Christie, 2000, Peptidyl boronic acids inhibit the degradation of presenilin-1 in presenilin-1 transfected cells, Alzheimer's Reports, 3, 169

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Metadata

Authors
Riddell, D. R., Christie, G., Hussain, I., & Dingwall, C.
Venue
Current Biology
Publisher
Elsevier BV
Published
2001-08
Volume
11
Issue
16
Pages
1288-1293
ISSN
0960-9822
DOI
10.1016/s0960-9822(01)00394-3
Cited by
253
References
1
Language
en
Licence

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