High-Throughput Analysis of Intact Human Proteins Using UVPD and HCD on an Orbitrap Mass Spectrometer
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The analysis of intact proteins (top-down strategy) by mass spectrometry has great potential to elucidate proteoform variation, including patterns of post-translational modifications (PTMs), which may not be discernible by analysis of peptides alone (bottom-up approach). To maximize sequence coverage and localization of PTMs, various fragmentation modes have been developed to produce fragment ions from deep within intact proteins. Ultraviolet photodissociation (UVPD) has recently been shown to produce high sequence coverage and PTM retention on a variety of proteins, with increasing evidence of efficacy on a chromatographic time scale. However, utilization of UVPD for high-throughput top-down analysis to date has been limited by bioinformatics. Here we detected 153 proteins and 489 proteoforms using UVPD and 271 proteins and 982 proteoforms using higher energy collisional dissociation (HCD) in a comparative analysis of HeLa whole-cell lysate by qualitative top-down proteomics. Of the total detected proteoforms, 286 overlapped between the UVPD and HCD data sets, with 68% of proteoforms having C scores greater than 40 for UVPD and 63% for HCD. The average sequence coverage (28 ± 20% for UVPD versus 17 ± 8% for HCD, p < 0.0001) was found to be higher for UVPD than HCD and with a trend toward improvement in q value for the UVPD data set. This study demonstrates the complementarity of UVPD and HCD for more extensive protein profiling and proteoform characterization.
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UVPD's diversity of bond breakages makes it ideal for deep sequence coverage, but higher numbers of protein identifications are still obtained using HCD for global top-down analysis by LC-MS.
“The diversity of bond breakages afforded by UVPD makes it ideal for deep sequence coverage, but higher numbers of protein identifications are still obtained using HCD for global top-down analysis by LC-MS [117].”
From Top-down proteomics: challenges, innovations, and applications in basic and clinical research (2020) · cites this paper as [117]
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- Authors
- Cleland, T. P., DeHart, C. J., Fellers, R. T., VanNispen, A. J., Greer, J. B., LeDuc, R. D., Parker, W. R., Thomas, P. M., Kelleher, N. L., & Brodbelt, J. S.
- Affiliations
- The University of Texas at Austin (US)
- Northwestern University (US)
- Corresponding author
- Brodbelt, Jennifer S.
- ORCID
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-04-19
- Volume
- 16
- Issue
- 5
- Pages
- 2072-2079
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- PMC
- PMC5549616
- OpenAlex
- W2607142718
- Cited by
- 96
- References
- 49
- Citations by year
- 2026: 8 · 2025: 2 · 2024: 8 · 2023: 5 · 2022: 12 · 2021: 7 · 2020: 8 · 2019: 21 · 2018: 19 · 2017: 5 · 2015: 1
- FWCI
- 7.15
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Smithsonian Digital Repository (Smithsonian Institution) (submittedVersion)
- Funders
- Division of Chemistry (CHE-1402753)
- Welch Foundation (F-1155)
- National Institute of General Medical Sciences (1K12GM102745, P41GM108569)
- UT System
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- top-down proteomics, ultraviolet photodissociation, higher-energy collisional dissociation, proteoforms, post-translational modification, sequencing depth, Orbitrap mass spectrometer, high-throughput proteomics
- MeSH terms
- HeLa Cells, Humans, Protein Processing, Post-Translational, Proteins, Ultraviolet Rays, Proteomics, Tandem Mass Spectrometry, High-Throughput Screening Assays
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