Chondrocyte-Targeted MicroRNA Delivery by Engineered Exosomes toward a Cell-Free Osteoarthritis Therapy
Abstract
Targeted delivery to the diseased cell or tissue is the key to the successful clinical use of nucleic acid drugs. In particular, delivery of microRNA-140 (miRNA-140, miR-140) into chondrocytes across the dense, nonvascular extracellular matrix of cartilage remains a major challenge. Here, we report the chondrocyte-targeting exosomes as vehicles for the delivery of miR-140 into chondrocytes as a new treatment for osteoarthritis (OA). By fusing a chondrocyte-affinity peptide (CAP) with the lysosome-associated membrane glycoprotein 2b protein on the surface of exosomes, we acquire CAP-exosomes that can efficiently encapsulate miR-140, specifically enter, and deliver the cargo into chondrocytes in vitro. CAP-exosomes, in contrast to nontagged exosome vesicles, are retained in the joints after intra-articular injection with minimal diffusion in vivo. CAP-exosomes also deliver miR-140 to deep cartilage regions through the dense mesochondrium, inhibit cartilage-degrading proteases, and alleviate OA progression in a rat model, pointing toward a potential organelle-based, cell-free therapy of OA.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Exosomes can be genetically engineered, chemically surface modified or enzymatically engineered to introduce peptides and proteins to enhance their targeting ability.
“In addition, exosomes can be genetically engineered, chemically surface modified or enzymatically engineered to introduce peptides and proteins to enhance their targeting ability [42, 83–86].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [84]
Cites
The biology <b>,</b> function <b>,</b> and biomedical applications of exosomes
Kalluri, R., & LeBleu, V. S. (2020) · Science · doi:10.1126/science.aau6977 · Cited by 10,770 · see citing works
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766 · see citing works
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930 · see citing works
Ageing and the pathogenesis of osteoarthritis
Loeser, R. F., Collins, J. A., & Diekman, B. O. (2016) · Nature Reviews Rheumatology · doi:10.1038/nrrheum.2016.65 · Cited by 1,309 · see citing works
Exosome Theranostics: Biology and Translational Medicine
He, C., Zheng, S., Luo, Y., & Wang, B. (2017) · Theranostics · doi:10.7150/thno.21945 · Cited by 1,259 · see citing works
Surface functionalized exosomes as targeted drug delivery vehicles for cerebral ischemia therapy
Tian, T., Zhang, H.-X., He, C.-P., Fan, S., Zhu, Y.-L., Qi, C., Huang, N.-P., Xiao, Z.-D., Lu, Z.-H., Tannous, B. A., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2017.10.012 · Cited by 1,154 · see citing works
Jo, C. H., Lee, Y. G., Shin, W. H., Kim, H., Chai, J. W., Jeong, E. C., Kim, J. E., Shim, H., Shin, J. S., Shin, I. S., et al. (2014) · Stem Cells · doi:10.1002/stem.1634 · Cited by 916 · see citing works
MicroRNA-140 plays dual roles in both cartilage development and homeostasis
Miyaki, S., Sato, T., Inoue, A., Otsuki, S., Ito, Y., Yokoyama, S., Kato, Y., Takemoto, F., Nakasa, T., Yamashita, S., et al. (2010) · Genes & Development · doi:10.1101/gad.1915510 · Cited by 660 · see citing works
Taylor, C. R., & Levenson, R. M. (2006) · Histopathology · doi:10.1111/j.1365-2559.2006.02513.x · Cited by 613 · see citing works
Re-Engineering Extracellular Vesicles as Smart Nanoscale Therapeutics
Armstrong, J. P. K., Holme, M. N., & Stevens, M. M. (2017) · ACS Nano · doi:10.1021/acsnano.6b07607 · Cited by 605 · see citing works
Progress in intra-articular therapy
Evans, C. H., Kraus, V. B., & Setton, L. A. (2013) · Nature Reviews Rheumatology · doi:10.1038/nrrheum.2013.159 · Cited by 584 · see citing works
The Role of MicroRNAs in Human Diseases.
Ardekani, A. M., & Naeini, M. M. (2010) · Europe PMC (PubMed Central) · Cited by 552 · see citing works
Exosomes for targeted siRNA delivery across biological barriers
Andaloussi, S. E., Lakhal‐Littleton, S., Mäger, I., & Wood, M. J. A. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.08.008 · Cited by 545 · see citing works
Chevalier, X., Goupille, P., Beaulieu, A. D., Burch, F. X., Bensen, W., Conrozier, T., Lœuille, D., Kivitz, A., Silver, D., & Appleton, B. E. (2009) · Arthritis Care & Research · doi:10.1002/art.24096 · Cited by 538 · see citing works
Intraarticular drug delivery in osteoarthritis☆
Gerwin, N., HOPS, C., & LUCKE, A. (2006) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2006.01.018 · Cited by 458 · see citing works
MicroRNA delivery through nanoparticles
Lee, S. W. L., Paoletti, C., Campisi, M., Osaki, T., Adriani, G., Kamm, R. D., Mattu, C., & Chiono, V. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.10.007 · Cited by 443 · see citing works
Exosomes: new molecular targets of diseases
Samanta, S., Rajasingh, S., Drosos, N., Zhou, Z., Dawn, B., & Rajasingh, J. (2017) · Acta Pharmacologica Sinica · doi:10.1038/aps.2017.162 · Cited by 427 · see citing works
Matas, J., Orrego, M., Amenábar, D., Infante, C., Tapia‐Limonchi, R., Cádiz, M. I., Alcayaga‐Miranda, F., González, P. L., Emilio Muse, R., Khoury, M., et al. (2018) · Stem Cells Translational Medicine · doi:10.1002/sctm.18-0053 · Cited by 425 · see citing works
Stabilization of Exosome-targeting Peptides via Engineered Glycosylation
Hung, M. E., & Leonard, J. N. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.621383 · Cited by 368 · see citing works
Li, Z., Zhou, X., Wei, M., Gao, X., Zhao, L., Shi, R., Sun, W., Duan, Y., Yang, G., & Yuan, L. (2019) · Nano Letters · doi:10.1021/acs.nanolett.8b02689 · Cited by 318 · see citing works
Baumann, V., & Winkler, J. (2014) · Future Medicinal Chemistry · doi:10.4155/fmc.14.116 · Cited by 300 · see citing works
Larsen, C., Østergaard, J., Larsen, S. W., Jensen, H. M., Jacobsen, S. E., Lindegaard, C., & Andersen, P. H. (2008) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.21346 · Cited by 299 · see citing works
Dendritic Cell–Derived Exosomes as Immunotherapies in the Fight against Cancer
Pitt, J. M., Charrier, M., Viaud, S., André, F., Besse, B., Chaput, N., & Zitvogel, L. (2014) · The Journal of Immunology · doi:10.4049/jimmunol.1400703 · Cited by 296 · see citing works
Cohen, S. B., Proudman, S., Kivitz, A., Burch, F. X., Donohue, J. P., Burstein, D., Sun, Y., Banfield, C. R., Vincent, M. S., Ni, L., et al. (2011) · Arthritis Research & Therapy · doi:10.1186/ar3430 · Cited by 249 · see citing works
Recent progress in microRNA-based delivery systems for the treatment of human disease
Fu, Y., Chen, J., & Huang, Z. (2019) · ExRNA · doi:10.1186/s41544-019-0024-y · Cited by 213 · see citing works
Chevalier, X., Giraudeau, B., Conrozier, T., Marliere, J., Kiefer, P., & Goupille, P. (2005) · PubMed · Cited by 197 · see citing works
Tapping the RNA world for therapeutics
Lieberman, J. (2018) · Nature Structural & Molecular Biology · doi:10.1038/s41594-018-0054-4 · Cited by 195 · see citing works
Transport of solutes through cartilage: permeability to large molecules.
Maroudas, A. (1976) · PubMed · Cited by 194 · see citing works
Si, H., Zeng, Y., Liu, S.-Y., Zhou, Z., Chen, Y., Cheng, J. Q., Lu, Y., & Shen, B. (2017) · Osteoarthritis and Cartilage · doi:10.1016/j.joca.2017.06.002 · Cited by 172 · see citing works
Clinical applications of microRNAs
Hydbring, P., & Badalian‐Very, G. (2013) · F1000Research · doi:10.12688/f1000research.2-136.v3 · Cited by 168 · see citing works
Establishing outcome measures in early knee osteoarthritis
Emery, C. A., Whittaker, J. L., Mahmoudian, A., Lohmander, S. L., Roos, E. M., Bennell, K. L., Toomey, C., Reimer, R. A., Thompson, D., Ronsky, J. L., et al. (2019) · Nature Reviews Rheumatology · doi:10.1038/s41584-019-0237-3 · Cited by 152 · see citing works
Cerebral Autoregulation in Awake Versus Isoflurane-Anesthetized Rats
Hoffman, W. E., Edelman, G. J., Kochs, E. F., Werner, C. D., Segil, L. J., & Albrecht, R. F. (1991) · Anesthesia & Analgesia · doi:10.1213/00000539-199112000-00013 · Cited by 109 · see citing works
Oseni, A. O., Butler, P. E. M., & Seifalian, A. M. (2012) · Journal of Surgical Research · doi:10.1016/j.jss.2012.05.087 · Cited by 101 · see citing works
MSC Therapy for Osteoarthritis: An Unfinished Story
Barry, F. (2019) · Journal of Orthopaedic Research® · doi:10.1002/jor.24343 · Cited by 100 · see citing works
Liang, Y., Duan, L., Xiong, J., Zhu, W., Liu, Q., Wang, D., Li, Z., Li, Z., & Wang, D. (2016) · Arthritis Research & Therapy · doi:10.1186/s13075-016-0997-y · Cited by 98 · see citing works
Self-citationmiR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence
Si, H.-B., Yang, T.-M., Li, L., Tian, M., Zhou, L., Li, D.-P., Huang, Q., Kang, P.-D., Yang, J., Zhou, Z.-K., et al. (2019) · Molecular Therapy: Nucleic Acids · doi:10.1016/j.omtn.2019.10.032 · Cited by 82 · see citing works
MicroRNA-140 and the silencing of osteoarthritisPreprint
Araldi, E., & Schipani, E. (2010) · Genes & Development · doi:10.1101/gad.1939310 · Cited by 74 · see citing works
Molecular therapy in the microRNA era
Würdinger, T., & Costa, F. F. (2006) · The Pharmacogenomics Journal · doi:10.1038/sj.tpj.6500429 · Cited by 71 · see citing works
Osteoarthritic cartilage loses its ability to remain avascular
Fenwick, S., GREGG, P. J., & Rooney, P. (1999) · Osteoarthritis and Cartilage · doi:10.1053/joca.1998.0238 · Cited by 59 · see citing works
Molecular mechanisms of the cartilage-specific microRNA-140 in osteoarthritis
Zhang, R., Ma, J., & Yao, J. (2013) · Inflammation Research · doi:10.1007/s00011-013-0654-8 · Cited by 59 · see citing works
DeWeerdt, S. (2019) · Nature · doi:10.1038/d41586-019-03068-4 · Cited by 34 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Engineering exosomes for targeted drug delivery
Liang, Y., Duan, L., Lu, J., & Xia, J. (2021) · Theranostics · doi:10.7150/thno.52570 · Cited by 1,547 · see citing works
Self-citationHybrid exosomes, exosome-like nanovesicles and engineered exosomes for therapeutic applications
Mondal, J., Pillarisetti, S., Junnuthula, V., Saha, M., Hwang, S. R., Park, I., & Lee, Y.-K. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.12.027 · Cited by 417 · see citing works
Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing
Duan, L., Ouyang, K., Xu, X. G., Xu, L., Wen, C., Zhou, X., Qin, Z., Xu, Z., Sun, W., & Liang, Y. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.673286 · Cited by 400 · see citing works
Self-citationExosome engineering in cell therapy and drug delivery
Sadeghi, S., Tehrani, F. R., Tahmasebi, S., Shafiee, A., & Hashemi, S. M. (2023) · Inflammopharmacology · doi:10.1007/s10787-022-01115-7 · Cited by 351 · see citing works
Exosomes, a New Star for Targeted Delivery
Chen, H., Wang, L., Zeng, X., Schwarz, H., Nanda, H. S., Peng, X., & Zhou, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.751079 · Cited by 345 · see citing works
Xu, G., Jin, J., Fu, Z., Wang, G., Lei, X., Xu, J., & Wang, J. (2025) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-025-02312-w · Cited by 309 · see citing works
Exosome-Based Drug Delivery: Translation from Bench to Clinic
Koh, H. B., Kim, H. J., Kang, S.-W., & Yoo, T.-H. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15082042 · Cited by 259 · see citing works
Exosome-mediated delivery of gene vectors for gene therapy
Duan, L., Xu, L., Xu, X., Qin, Z., Zhou, X., Xiao, Y., Liang, Y., & Xia, J. (2021) · Nanoscale · doi:10.1039/d0nr07622h · Cited by 238 · see citing works
Self-citationEngineered Exosome for Drug Delivery: Recent Development and Clinical Applications
Tian, J., Han, Z., Song, D., Peng, Y., Xiong, M., Chen, Z., Duan, S., & Zhang, L. (2023) · International Journal of Nanomedicine · doi:10.2147/ijn.s444582 · Cited by 239 · see citing works
Chondrocyte-specific genomic editing enabled by hybrid exosomes for osteoarthritis treatment
Liang, Y., Xu, X., Xu, L., Iqbal, Z., Ouyang, K., Zhang, H., Wen, C., Duan, L., & Xia, J. (2022) · Theranostics · doi:10.7150/thno.69368 · Cited by 217 · see citing works
Self-citationThe non-coding RNA interactome in joint health and disease
Ali, S. A., Peffers, M. J., Ormseth, M. J., Jurišica, I., & Kapoor, M. (2021) · Nature Reviews Rheumatology · doi:10.1038/s41584-021-00687-y · Cited by 213 · see citing works
Extracellular vesicles as a drug delivery system: A systematic review of preclinical studies
de Castilla, P. E. M., Tong, L., Huang, C., Sofias, A. M., Pastorin, G., Chen, X. S., Storm, G., Schiffelers, R. M., & Wang, J. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.05.011 · Cited by 214 · see citing works
Liu, Q., Li, D., Pan, X., & Liang, Y. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-02081-0 · Cited by 201 · see citing works
Self-citationExosomes as natural nanocarrier-based drug delivery system: recent insights and future perspectives
Sen, S., Xavier, J., Kumar, N., Ahmad, M. Z., & Ranjan, O. P. (2023) · 3 Biotech · doi:10.1007/s13205-023-03521-2 · Cited by 194 · see citing works
Injectable Drug Delivery Systems for Osteoarthritis and Rheumatoid Arthritis
Bruno, M. C., Cristiano, M. C., Celia, C., d’Avanzo, N., Mancuso, A., Paolino, D., Wolfram, J., & Fresta, M. (2022) · ACS Nano · doi:10.1021/acsnano.2c06393 · Cited by 176 · see citing works
Cao, H., Chen, M., Cui, X., Liu, Y., Liu, Y., Deng, S., Yuan, T., Fan, Y., Wang, Q., & Zhang, X. (2023) · ACS Nano · doi:10.1021/acsnano.3c01612 · Cited by 168 · see citing works
Harnessing Tissue-derived Extracellular Vesicles for Osteoarthritis Theranostics
Yin, B., Ni, J., Witherel, C. E., Yang, M., Burdick, J. A., Wen, C., & Wong, S. H. D. (2021) · Theranostics · doi:10.7150/thno.62708 · Cited by 167 · see citing works
Liang, Y., Iqbal, Z., Lu, J., Wang, J., Zhang, H., Chen, X., Duan, L., & Xia, J. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2022.10.008 · Cited by 162 · see citing works
Self-citationChen, M., Lu, Y., Liu, Y., Liu, Q., Deng, S., Liu, Y., Cui, X., Liang, J., Zhang, X., Fan, Y., et al. (2024) · Advanced Materials · doi:10.1002/adma.202312559 · Cited by 158 · see citing works
Rankin‐Turner, S., Vader, P., O’Driscoll, L., Giebel, B., Heaney, L. M., & Davies, O. G. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.04.012 · Cited by 155 · see citing works
Cell-free exosome-laden scaffolds for tissue repair
Huang, J., Xiong, J., Yang, L., Zhang, J., Sun, S., & Liang, Y. (2021) · Nanoscale · doi:10.1039/d1nr01314a · Cited by 152 · see citing works
Self-citationExosome for mRNA delivery: Strategies and therapeutic applications
Iqbal, Z., Rehman, K., Mahmood, A., Shabbir, M., Liang, Y., Duan, L., & Zeng, H. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02634-x · Cited by 144 · see citing works
Self-citationEngineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications
Wang, L., Wang, D., Ye, Z., & Xu, J. (2023) · Advanced Science · doi:10.1002/advs.202300552 · Cited by 133 · see citing works
Self-citationInsulin-like growth factor-1 in articular cartilage repair for osteoarthritis treatment
Wen, C., Xu, L., Xu, X. G., Wang, D., Liang, Y., & Duan, L. (2021) · Arthritis Research & Therapy · doi:10.1186/s13075-021-02662-0 · Cited by 130 · see citing works
Self-citationWu, Y., Li, J., Zeng, Y., Pu, W., Mu, X., Sun, K., Peng, Y., & Shen, B. (2022) · International Journal of Oral Science · doi:10.1038/s41368-022-00187-z · Cited by 124 · see citing works
Exosomes as smart drug delivery vehicles for cancer immunotherapy
Zhang, H., Wang, S., Sun, M., Cui, Y., Xing, J., Teng, L., Xi, Z., & Yang, Z. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2022.1093607 · Cited by 118 · see citing works
Ortega, A., Martínez-Arroyo, O., Forner, M. J., & Cortés, R. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics13010003 · Cited by 111 · see citing works
Zhao, S., Xiu, G., Wang, J., Wen, Y., Lu, J., Wu, B., Wang, G., Yang, D., Ling, B., Du, D., et al. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-02086-9 · Cited by 109 · see citing works
Research Advances of Engineered Exosomes as Drug Delivery Carrier
Huang, L., Wu, E., Liao, J., Wei, Z., Wang, J., & Chen, Z. (2023) · ACS Omega · doi:10.1021/acsomega.3c04479 · Cited by 107 · see citing works
Breakthrough of extracellular vesicles in pathogenesis, diagnosis and treatment of osteoarthritis
Liu, Z., Zhuang, Y., Fang, L., Yuan, C., Wang, X., & Lin, K. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2022.10.012 · Cited by 100 · see citing works
Programming assembly of biomimetic exosomes: An emerging theranostic nanomedicine platform
Xu, X., Xu, L., Wen, C., Xia, J., Zhang, Y., & Liang, Y. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100760 · Cited by 101 · see citing works
Self-citation3D Bioprinting of Hydrogels for Cartilage Tissue Engineering
Huang, J., Xiong, J., Wang, D., Zhang, J., Yang, L., Sun, S., & Liang, Y. (2021) · Gels · doi:10.3390/gels7030144 · Cited by 97 · see citing works
Self-citationSong, H., Chen, X., Hao, Y., Wang, J., Xie, Q., & Wang, X. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01638-9 · Cited by 96 · see citing works
Ahmadi, S. E., Soleymani, M., Shahriyary, F., Amirzargar, M. R., Mahya, O., Fattahi, D., & Safa, M. (2023) · Cancer Gene Therapy · doi:10.1038/s41417-023-00597-z · Cited by 95 · see citing works
Liu, H., Geng, Z., & Su, J. (2022) · Extracellular Vesicles and Circulating Nucleic Acids · doi:10.20517/evcna.2022.04 · Cited by 94 · see citing works
Li, S., Stöckl, S., Lukas, C., Herrmann, M., Brochhausen, C., König, M. A., Johnstone, B., & Grässel, S. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02317-6 · Cited by 96 · see citing works
Bone-targeted biomaterials: Strategies and applications
Ren, X., Chen, X., Geng, Z., & Su, J. (2022) · Chemical Engineering Journal · doi:10.1016/j.cej.2022.137133 · Cited by 92 · see citing works
Wang, C., Xu, M., Fan, Q., Li, C., & Zhou, X. (2022) · Asian Journal of Pharmaceutical Sciences · doi:10.1016/j.ajps.2022.100772 · Cited by 91 · see citing works
Akbari, A., Nazari‐Khanamiri, F., Ahmadi, M., Shoaran, M., & Rezaie, J. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics15010066 · Cited by 89 · see citing works
Zhang, H., Yan, W.-J., Wang, J., Xie, S.-Q., Tao, W., Lee, C.-W., Zhang, X., Zhang, G.-Y., Liu, Y.-F., Wei, D., et al. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.04.009 · Cited by 88 · see citing works
Self-citationBone‐Targeted Exosomes: Strategies and Applications
Wang, J., Li, X., Wang, S., Cui, J., Ren, X., & Su, J. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202203361 · Cited by 88 · see citing works
Self-citationHarnessing knee joint resident mesenchymal stem cells in cartilage tissue engineering
Xu, X. G., Xu, L., Xia, J., Wen, C., Liang, Y., & Zhang, Y. (2023) · Acta Biomaterialia · doi:10.1016/j.actbio.2023.07.024 · Cited by 92 · see citing works
Self-citationKnee Osteoarthritis Therapy: Recent Advances in Intra-Articular Drug Delivery Systems
Ma, L., Zheng, X., Lin, R., Sun, A. R., Song, J., Ye, Z., Liang, D., Zhang, M., Tian, J. H., Zhou, X., et al. (2022) · Drug Design Development and Therapy · doi:10.2147/dddt.s357386 · Cited by 86 · see citing works
Exosome-based drug delivery systems and their therapeutic applications
Lee, J., Lee, J.-H., Chakraborty, K., Hwang, J., & Lee, Y.-K. (2022) · RSC Advances · doi:10.1039/d2ra02351b · Cited by 85 · see citing works
Understanding exosomes: Part 2—Emerging leaders in regenerative medicine
Miron, R. J., Estrin, N. E., Sculean, A., & Zhang, Y. (2024) · Periodontology 2000 · doi:10.1111/prd.12561 · Cited by 83 · see citing works
Sharma, A., Yadav, A., Nandy, A., & Ghatak, S. (2024) · Pharmaceutics · doi:10.3390/pharmaceutics16060709 · Cited by 83 · see citing works
Han, J., Sul, J. H., Lee, J., Kim, E., Kim, H. K., Chae, M., Lim, J., Kim, J., Kim, C. H., Kim, J. S., et al. (2024) · Science Translational Medicine · doi:10.1126/scitranslmed.adi4830 · Cited by 85 · see citing works
Exosomes as Targeted Delivery Platform of CRISPR/Cas9 for Therapeutic Genome Editing
Duan, L., Ouyang, K., Wang, J., Xu, L., Xu, X., Wen, C., Xie, Y., Liang, Y., & Xia, J. (2021) · ChemBioChem · doi:10.1002/cbic.202100359 · Cited by 82 · see citing works
Self-citationIntra-articular nanodrug delivery strategies for treating osteoarthritis
Zhou, D., Zhou, F., Sheng, S., Wei, Y., Chen, X., & Su, J. (2022) · Drug Discovery Today · doi:10.1016/j.drudis.2022.103482 · Cited by 81 · see citing works
Zhang, X. Y., Jiang, Y., Huang, Q., Wu, Z., Pu, H., Xu, Z., Li, B., Lu, X., Yang, X., Qin, J., et al. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02475-7 · Cited by 80 · see citing works
Self-citationEngineering approaches for RNA-based and cell-based osteoarthritis therapies
DeJulius, C. R., Walton, B. L., Colazo, J. M., d’Arcy, R., Francini, N., Brunger, J. M., & Duvall, C. L. (2024) · Nature Reviews Rheumatology · doi:10.1038/s41584-023-01067-4 · Cited by 81 · see citing works
Liu, H., Zhang, H., Wang, S., Cui, J., Weng, W., Liu, X., Tang, H., Hu, Y., Li, X., Zhang, K., et al. (2023) · Composites Part B Engineering · doi:10.1016/j.compositesb.2023.110610 · Cited by 78 · see citing works
Self-citationBiodistribution of therapeutic extracellular vesicles
Gupta, D., Wiklander, O. P. B., Wood, M. J. A., & Andaloussi, S. E. (2023) · Extracellular Vesicles and Circulating Nucleic Acids · doi:10.20517/evcna.2023.12 · Cited by 82 · see citing works
Song, Z., Tao, Y., Liu, Y., & Li, J. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1444437 · Cited by 79 · see citing works
Lin, Z., Xu, G., Lu, X., Liu, S., Zou, F., Ma, X., Jiang, J., Wang, H., & Song, J. G. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02517-1 · Cited by 76 · see citing works
Plant-derived exosomal nanoparticles: potential therapeutic for inflammatory bowel disease
Li, D., Tang, Q., Yang, M., Xu, H., Zhu, M., Zhang, Y., Tian, C., Nie, Y., Wang, J., Liang, Y., et al. (2023) · Nanoscale Advances · doi:10.1039/d3na00093a · Cited by 78 · see citing works
Self-citationNanomaterials for mRNA ‐based therapeutics: Challenges and opportunities
Li, D., Liu, Q., Yang, M., Xu, H., Zhu, M., Zhang, Y., Xu, J., Tian, C., Yao, J., Wang, L., et al. (2023) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10492 · Cited by 71 · see citing works
Self-citationLiang, Y., Iqbal, Z., Wang, J., Xu, L., Xu, X., Ouyang, K., Zhang, H., Lu, J., Duan, L., & Xia, J. (2022) · Biomaterials Science · doi:10.1039/d2bm00480a · Cited by 72 · see citing works
Self-citationCurrent Nanoparticle-Based Technologies for Osteoarthritis Therapy
Jin, G.-Z. (2020) · Nanomaterials · doi:10.3390/nano10122368 · Cited by 64 · see citing works
Engineered extracellular vesicles for bone therapy
Jiang, Y., Li, J., Xu, X. J., Yin, Z., Xu, K., & Su, J. (2022) · Nano Today · doi:10.1016/j.nantod.2022.101487 · Cited by 68 · see citing works
Self-citationMitochondria-targeting sonosensitizer-loaded extracellular vesicles for chemo-sonodynamic therapy
Nguyen Cao, T. G., Truong Hoang, Q., Hong, E. J., Kang, S. J., Kang, J. H., Ravichandran, V., Kang, H. C., Ko, Y. T., Rhee, W. J., & Shim, M. S. (2023) · Journal of Controlled Release · doi:10.1016/j.jconrel.2023.01.044 · Cited by 69 · see citing works
Namini, M. S., Daneshimehr, F., Beheshtizadeh, N., Mansouri, V., Ai, J., Jahromi, H. K., & Ebrahimi‐Barough, S. (2023) · Stem Cell Research & Therapy · doi:10.1186/s13287-023-03467-5 · Cited by 72 · see citing works
Engineered Extracellular Vesicles as a Targeted Delivery Platform for Precision Therapy
Sun, Y., Sun, F., Xu, W., & Qian, H. (2023) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-022-00503-y · Cited by 69 · see citing works
Cartilage-targeted drug nanocarriers for osteoarthritis therapy
Morici, L., Allémann, É., Rodríguez‐Nogales, C., & Jordan, O. (2024) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2024.124843 · Cited by 72 · see citing works
Osteoclast-targeted delivery of anti-miRNA oligonucleotides by red blood cell extracellular vesicles
Xu, L., Xu, X., Liang, Y., Wen, C., Ouyang, K., Huang, J., Xiao, Y., Deng, X., Xia, J., & Duan, L. (2023) · Journal of Controlled Release · doi:10.1016/j.jconrel.2023.04.043 · Cited by 65 · see citing works
Self-citationTang, J., Wang, X., Lin, X., & Wu, C. (2024) · Cell Death Discovery · doi:10.1038/s41420-024-01973-w · Cited by 69 · see citing works
Liu, X., Zhang, L., Xu, Z., Xiong, X., Yu, Y., Wu, H., Qiao, H., Zhong, J., Zhao, Z., Dai, J., et al. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.10.027 · Cited by 65 · see citing works
Mesenchymal stem cell-derived exosomes as a promising cell-free therapy for knee osteoarthritis
Luo, D., Zhu, H., Li, S., Wang, Z., & Xiao, J. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1309946 · Cited by 64 · see citing works
Xu, S., Chang, L., Zhao, X., Hu, Y., Lin, Y., Chen, Z., Ren, X., & Mei, X. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.03.038 · Cited by 62 · see citing works
Nguyen, T. H., Duong, C. M., Nguyen, X., & Than, U. T. T. (2021) · Cells · doi:10.3390/cells10112887 · Cited by 62 · see citing works
Emerging role of bacterial outer membrane vesicle in gastrointestinal tract
Tian, C., Yang, M., Xu, H., Zhu, M., Zhang, Y., Yao, J., Wang, L., Liang, Y.-J., & Li, D. (2023) · Gut Pathogens · doi:10.1186/s13099-023-00543-2 · Cited by 61 · see citing works
Self-citationAdvanced Nanocomposite Hydrogels for Cartilage Tissue Engineering
Huang, J., Liu, F., Su, H., Xiong, J., Yang, L., Xia, J., & Liang, Y. (2022) · Gels · doi:10.3390/gels8020138 · Cited by 57 · see citing works
Self-citationCharge‐Reversed Exosomes for Targeted Gene Delivery to Cartilage for Osteoarthritis Treatment
Zhang, C., Pathrikar, T. V., Baby, H. M., Li, J., Zhang, H., Selvadoss, A., Ovchinnikova, A., Ionescu, A. M., Chubinskaya, S., Miller, R. E., et al. (2024) · Small Methods · doi:10.1002/smtd.202301443 · Cited by 57 · see citing works
Osteoarthritis year in review: genetics, genomics, epigenetics
Young, D. A., Barter, M. J., & Soul, J. (2021) · Osteoarthritis and Cartilage · doi:10.1016/j.joca.2021.11.004 · Cited by 56 · see citing works
Yin, Z., Qin, C., Pan, S., Shi, C., Wu, G., Feng, Y., Zhang, J., Yu, Z., Liang, B., & Gui, J. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100813 · Cited by 56 · see citing works
Tissue Engineering and Regenerative Medicine: Perspectives and Challenges
Hoang, V. T., Nguyen, Q. T., Phan, T. T. K., Pham, T. H., Dinh, N. T. H., Anh, L. P. H., Dao, L. T. M., Bui, V. D., Dao, H., Le, D. S., et al. (2025) · MedComm · doi:10.1002/mco2.70192 · Cited by 62 · see citing works
Deng, S., Cao, H., Cui, X., Fan, Y., Wang, Q., & Zhang, X. (2023) · Acta Biomaterialia · doi:10.1016/j.actbio.2023.09.023 · Cited by 56 · see citing works
Bone Targeting Nanoparticles for the Treatment of Osteoporosis
Wen, C., Xu, X. M., Zhang, Y., Xia, J., Liang, Y., & Xu, L. (2024) · International Journal of Nanomedicine · doi:10.2147/ijn.s444347 · Cited by 58 · see citing works
Self-citationNon-surgical osteoarthritis therapy, intra-articular drug delivery towards clinical applications
Liang, Y.-J., Xu, X., Xu, L., Prasadam, I., Duan, L., Xiao, Y., & Xia, J. (2020) · Journal of drug targeting (Print) · doi:10.1080/1061186x.2020.1870231 · Cited by 46 · see citing works
Self-citationBiomaterial‐based gene therapy
Yu, Y., Gao, Y., He, L., Fang, B., Ge, W., Pu, Y., Ju, Y., Xie, X., & Lei, L. (2023) · MedComm · doi:10.1002/mco2.259 · Cited by 53 · see citing works
Exosome: From biology to drug delivery
Baruah, H., Sarma, A., Basak, D., & Das, M. (2024) · Drug Delivery and Translational Research · doi:10.1007/s13346-024-01515-y · Cited by 54 · see citing works
Ahmed, W., Mushtaq, A., Ali, S., Khan, N., Liang, Y., & Duan, L. (2024) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.4c00856 · Cited by 54 · see citing works
Self-citationMei, Z.-X., Yilamu, K., Ni, W., Shen, P., Pan, N., Chen, H., Su, Y., Guo, L., Sun, Q., Li, Z., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-60037-4 · Cited by 55 · see citing works
Advanced 3D-Printing Bioinks for Articular Cartilage Repair
Liang, Q., Ma, Y., Yao, X., & Wei, W. (2022) · International Journal of Bioprinting · doi:10.18063/ijb.v8i3.511 · Cited by 55 · see citing works
Wen, C., Lin, L., Zou, R., Lin, F., & Liu, Y. (2021) · Cell Cycle · doi:10.1080/15384101.2021.2019411 · Cited by 52 · see citing works
Rao, Q., Ma, G., Li, M., Wu, H., Zhang, Y., Zhang, C.-E., Ma, Z., & Huang, L. (2022) · British Journal of Pharmacology · doi:10.1111/bph.15958 · Cited by 53 · see citing works
Hadizadeh, N., Bagheri, D., Shamsara, M., Hamblin, M. R., Farmany, A., Xu, M., Liang, Z., Razi, F., & Hashemi, E. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1019821 · Cited by 49 · see citing works
Tracking tools of extracellular vesicles for biomedical research
Liu, Q., Huang, J., Xia, J., Liang, Y., & Li, G. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.943712 · Cited by 54 · see citing works
Self-citationEngineering extracellular vesicles to deliver CRISPR ribonucleoprotein for gene editing
Whitley, J. A., & Cai, H. (2023) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12343 · Cited by 54 · see citing works
Potential of Exosomes as Cell-Free Therapy in Articular Cartilage Regeneration: A Review
Ng, C. Y., Chai, J. Y., Foo, J. B., Yahaya, N. H. M., Yang, Y., Ng, M. H., & Law, J. X. (2021) · International Journal of Nanomedicine · doi:10.2147/ijn.s327059 · Cited by 51 · see citing works
Native and engineered exosomes for inflammatory disease
Ma, X., Liu, B., Fan, L., Liu, Y., Zhao, Y., Ren, T., Li, Y., & Li, Y. (2022) · Nano Research · doi:10.1007/s12274-022-5275-5 · Cited by 50 · see citing works
Yang, Y., & Zhang, H. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202401547 · Cited by 51 · see citing works
Householder, N. A., Raghuram, A. K., Agyare, K., Thipaphay, S., & Zumwalt, M. A. (2023) · Orthopaedic Journal of Sports Medicine · doi:10.1177/23259671231155950 · Cited by 49 · see citing works
Recent progresses of exosome–liposome fusions in drug delivery
Zheng, L., Hu, B., Zhao, D., Liu, W., Liu, Q., Huang, Y., & Ruan, S. (2023) · Chinese Chemical Letters · doi:10.1016/j.cclet.2023.108647 · Cited by 49 · see citing works
Fan, X., Zhang, Y., Liu, W., Shao, M., Gong, Y., Wang, T., Xue, S., & Nian, R. (2024) · Biomaterials Science · doi:10.1039/d4bm00558a · Cited by 49 · see citing works
Challenges in osteoarthritis treatment
Emami, A., Namdari, H., Parvizpour, F., & Arabpour, Z. (2022) · Tissue and Cell · doi:10.1016/j.tice.2022.101992 · Cited by 48 · see citing works
Engineered exosomes as drug and RNA co-delivery system: new hope for enhanced therapeutics?
Chen, H., Yao, H., Chi, J., Li, C., Liu, Y., Jiayi, Y., Yu, J., Wang, J., Ruan, Y., Pi, J., et al. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1254356 · Cited by 47 · see citing works
Biomimetic and Bioinspired Intervention Strategies for the Treatment of Rheumatoid Arthritis
Han, Y., Pang, X., & Pi, G. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202104640 · Cited by 48 · see citing works
Modification of Extracellular Vesicle Surfaces: An Approach for Targeted Drug Delivery
Mohammadi, A. H., Ghazvinian, Z., Bagheri, F., Harada, M., & Baghaei, K. (2023) · BioDrugs · doi:10.1007/s40259-023-00595-5 · Cited by 47 · see citing works
Zeng, B., Li, Y., Xia, J., Xiao, Y., Khan, N., Jiang, B., Liang, Y., & Duan, L. (2024) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10623 · Cited by 45 · see citing works
Self-citation
- Authors
- Liang, Y., Xu, X., Li, X., Xiong, J., Li, B., Duan, L., Wang, D., & Xia, J.
- Affiliations
- Chinese University of Hong Kong (HK)
- Shenzhen University Health Science Center (CN)
- Southern University of Science and Technology (CN)
- Corresponding author
- Duan, Li
- Wang, Daping
- Xia, Jiang
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-07-30
- Volume
- 12
- Issue
- 33
- Pages
- 36938-36947
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W3045995463
- Cited by
- 391
- References
- 41
- Citations by year
- 2026: 52 · 2025: 77 · 2024: 78 · 2023: 79 · 2022: 62 · 2021: 38 · 2020: 5
- FWCI
- 14.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Research Grants Council, University Grants Committee (14306317, 14307218, AoE/M-09/12, N_CUHK422/18)
- Ministry of Science and Technology of the People's Republic of China (2018YFA0903204)
- National Natural Science Foundation of China (81772394, 81972085, 81972116)
- Natural Science Foundation of Guangdong Province (2018B0303110003)
- Chinese University of Hong Kong
- Shenzhen Science and Technology Projects (JCYJ20170306092215436, JCYJ20170413161649437, JCYJ20170817172023838, KQTD20170331100838136)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- microRNA-140, exosomes, osteoarthritis, cell-free therapy, Lamp2b, cartilage, intra-articular injection, nucleic acid drug delivery
- MeSH terms
- Animals, Cartilage, Articular, Cells, Cultured, Extracellular Matrix, Gene Expression Regulation, Humans, Male, Membrane Glycoproteins, Osteoarthritis, Peptides, Genetic Therapy, Rats, Sprague-Dawley, Gene Transfer Techniques, Chondrocytes, MicroRNAs, Rats, Exosomes, Molecular Targeted Therapy, Optical Imaging
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
