Cited by 1,154Extracellular vesicles in diseaseNanoparticle-Based Drug DeliveryBarrier Structure and Function Studies

Surface functionalized exosomes as targeted drug delivery vehicles for cerebral ischemia therapy

Tian, Tian, Zhang, Hui-Xin, He, Chun-Peng, Fan, Song, Zhu, Yan-Liang, Qi, Cui, Huang, Ning-Ping, Xiao, Zhong-Dang +3 more·2018·Biomaterials

Abstract

The safe and effective delivery of drugs is a major obstacle in the treatment of ischemic stroke. Exosomes hold great promise as an endogenous drug delivery nanosystem for the treatment of cerebral ischemia given their unique properties, including low immunogenicity, innate stability, high delivery efficiency, and ability to cross the blood-brain barrier (BBB). However, exosome insufficient targeting capability limits their clinical applications. In this study, the c(RGDyK) peptide has been conjugated to the exosome surface by an easy, rapid, and bio-orthogonal chemistry. In the transient middle cerebral artery occlusion (MCAO) mice model, The engineered c(RGDyK)-conjugated exosomes (cRGD-Exo) target the lesion region of the ischemic brain after intravenous administration. Furthermore, curcumin has been loaded onto the cRGD-Exo, and administration of these exosomes has resulted in a strong suppression of the inflammatory response and cellular apoptosis in the lesion region. The results suggest a targeting delivery vehicle for ischemic brain based on exosomes and provide a strategy for the rapid and large-scale production of functionalized exosomes.

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Crossref · 10.1016/j.biomaterials.2017.10.012

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Authors
Tian, T., Zhang, H.-X., He, C.-P., Fan, S., Zhu, Y.-L., Qi, C., Huang, N.-P., Xiao, Z.-D., Lu, Z.-H., Tannous, B. A., & Gao, J.
Affiliations
  • Harvard University (US)
  • Sun Yat-sen University (CN)
  • Nanjing Medical University (CN)
  • State Key Laboratory of Digital Medical Engineering (CN)
Corresponding author
Gao, Jun
Venue
Biomaterials
Publisher
Elsevier BV
Published
2018-01
Volume
150
Pages
137-149
ISSN
0142-9612 (ISSN-L)
OpenAlex
W2761425229
Cited by
1,154
References
72
Citations by year
2026: 98 · 2025: 146 · 2024: 182 · 2023: 186 · 2022: 173 · 2021: 150 · 2020: 108 · 2019: 77 · 2018: 32 · 2017: 1
FWCI
24.34
Type
journal article
Language
en
Open access
closed
Locations
  • Biomaterials (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81222013, 81673416, 81401092)
  • Jiangsu Province (BE2016761)
  • Natural Science Foundation of Jiangsu Province (BK20170107, BK20130886)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
exosomes, surface functionalization, targeted drug delivery, cerebral ischemia, ischemic stroke, RGD peptide conjugation, bioorthogonal chemistry, curcumin, middle cerebral artery occlusion, blood-brain barrier, neuroinflammation, apoptosis, exosome production
MeSH terms
Animals, Curcumin, Disease Models, Animal, HeLa Cells, Humans, Injections, Intravenous, Male, Mice, Inbred C57BL, Peptides, Pharmaceutical Vehicles, Infarction, Middle Cerebral Artery, Stroke, Cell Line, Tumor, Mice, Exosomes

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