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Liposomes from ionic, single-chain amphiphiles
Abstract
In studies of the minimum physiochemical requirements for lipid membrane formation, we have made liposomes from dilute, aqueous dispersions of C(8)-C(18) single-chain amphiphiles. In general, membrane formation from ionic soaps and detergents requires the presence of uncharged amphiphiles. Vesicles were characterized by phase-contrast microscopy, by trapping of ionic dyes, as well as by negativestain and freez-frature electron microscopy. They were typically heterogeneous in size, but the average diameter could be experimentally varied in some cases over the range of 1 to 100 micrometer. Uni-, oligo-, and multilamellar vesicles were observed. Membrane permeability to various solutes was determined in part by a new technique which utilized phase-contract microscopy; when impermeable vesciles exclude added solutes such as sucrose, refractive index differences are created between vesicle contents and surrounding medium, so that the vesicles appear bright in the phase microscope. Permeant solutes do not produce this effect. Spectrophotometric permeability determinations confirmed the results of this technique and provided quantitative measures of permeability. Monoalkyl liposomes have potential uses as models of biomembranes and in drug delivery. They are also relevant to the prebiotic origin of biomembranes.
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Apel, C. L., Deamer, D. W., & Mautner, M. N. (2002) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(01)00400-x · Cited by 350
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Surfactant vesicles as membrane mimetic agents: characterization and utilization
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Schrum, J. P., Zhu, T. F., & Szostak, J. W. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a002212 · Cited by 255
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Chen, I. A., & Szostak, J. W. (2004) · Biophysical Journal · doi:10.1529/biophysj.104.039875 · Cited by 251
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Jordan, S. F., Rammu, H., Zheludev, I. N., Hartley, A. M., Maréchal, A., & Lane, N. (2019) · Nature Ecology & Evolution · doi:10.1038/s41559-019-1015-y · Cited by 178
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Self-citation
Metadata
- Authors
- Hargreaves, W. R., & Deamer, D. W.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1978-09-05
- Volume
- 17
- Issue
- 18
- Pages
- 3759-3768
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2073934614
- Cited by
- 575
- References
- 0
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
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