Cited by 237Hydrogels: synthesis, properties, applications3D Printing in Biomedical ResearchCellular Mechanics and Interactions

Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis

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Chrisnandy, Antonius, Blondel, Delphine, Rezakhani, Saba, Broguiere, Nicolas, Lutolf, Matthias P.·2022·Nature Materials

Abstract

Epithelial organoids are most efficiently grown from mouse-tumour-derived, reconstituted extracellular matrix hydrogels, whose poorly defined composition, batch-to-batch variability and immunogenicity limit clinical applications. Efforts to replace such ill-defined matrices for organoid culture have largely focused on non-adaptable hydrogels composed of covalently crosslinked hydrophilic macromolecules. However, the excessive forces caused by tissue expansion in such elastic gels severely restrict organoid growth and morphogenesis. Chemical or enzymatic degradation schemes can partially alleviate this problem, but due to their irreversibility, long-term applicability is limited. Here we report a family of synthetic hydrogels that promote extensive organoid morphogenesis through dynamic rearrangements mediated by reversible hydrogen bonding. These tunable matrices are stress relaxing and thus promote efficient crypt budding in intestinal stem-cell epithelia through increased symmetry breaking and Paneth cell formation dependent on yes-associated protein 1. As such, these well-defined gels provide promising versatile matrices for fostering elaborate in vitro morphogenesis.

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Crossref · 10.1038/s41563-021-01136-7

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  • Adult stem cell activity can be controlled using PEG hydrogels with photo-degradable moieties.

    “the activity of adult stem cells can be controlled using PEG hydrogels with photo-degradable moieties67”

    From Organoids (2022) · cites this paper as [67]

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Authors
Chrisnandy, A., Blondel, D., Rezakhani, S., Broguiere, N., & Lutolf, M. P.
Affiliations
École Polytechnique Fédérale de Lausanne (CH)
Venue
Nature Materials
Published
2021-11-15
Volume
21
Issue
4
Pages
479-487
ISSN
  • 1476-1122 (ISSN-L)
  • 1476-4660
OpenAlex
W3211744428
Cited by
237
References
53
Citations by year
2026: 32 · 2025: 59 · 2024: 54 · 2023: 55 · 2022: 35 · 2021: 2
FWCI
11.52
Type
journal article
Language
en
Open access
Locations
  • Nature Materials (publishedVersion)
  • PubMed (publishedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
synthetic hydrogels, dynamic hydrogels, stress relaxation, intestinal organoids, symmetry breaking, Paneth cells, YAP, extracellular matrix, tissue expansion
MeSH terms
Animals, Extracellular Matrix, Organoids, Stem Cells, Hydrogels, Organogenesis, Mice

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