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Cited by 183Luminescence and Fluorescent MaterialsMolecular Sensors and Ion DetectionOptical Imaging and Spectroscopy Techniques

Bright Chromenylium Polymethine Dyes Enable Fast, Four-Color In Vivo Imaging with Shortwave Infrared Detection

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Cosco, Emily D., Arús, Bernardo A., Spearman, Anthony L., Atallah, Timothy L., Lim, Irene, Leland, Olivia S., Caram, Justin R., Bischof, Thomas S. +2 more·2021·Journal of the American Chemical Society
DOI 10.1021/jacs.0c11599PMC PMC8327756
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Abstract

High Resolution Image Download MS PowerPoint Slide Optical imaging within the shortwave infrared (SWIR, 1000–2000 nm) region of the electromagnetic spectrum has enabled high-resolution and high-contrast imaging in mice, non-invasively. Polymethine dyes, with their narrow absorption spectra and high absorption coefficients, are optimal probes for fast and multiplexed SWIR imaging. Here, we expand upon the multiplexing capabilities in SWIR imaging by obtaining brighter polymethine dyes with varied excitation wavelengths spaced throughout the near-infrared (700–1000 nm) region. Building on the flavylium polymethine dye scaffold, we explored derivatives with functional group substitution at the 2-position, deemed chromenylium polymethine dyes. The reported dyes have reduced nonradiative rates and enhanced emissive properties, enabling non-invasive imaging in mice in a single color at 300 fps and in three colors at 100 fps. Combined with polymethine dyes containing a red-shifted julolidine flavylium heterocycle and indocyanine green, distinct channels with well-separated excitation wavelengths provide non-invasive video-rate in vivo imaging in four colors.

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Authors
Cosco, E. D., Arús, B. A., Spearman, A. L., Atallah, T. L., Lim, I., Leland, O. S., Caram, J. R., Bischof, T. S., Bruns, O. T., & Sletten, E. M.
Affiliations
  • University of California, Los Angeles (US)
  • Helmholtz Munich (DE)
  • Helmholtz Pioneer Campus, Helmholtz Zentrum München, D-85764 Neuherberg, Germany
Corresponding author
Sletten, Ellen M.
ORCID
Venue
Journal of the American Chemical Society
Publisher
American Chemical Society (ACS)
Published
2021-05-03
Volume
143
Issue
18
Pages
6836-6846
ISSN
  • 0002-7863 (ISSN-L)
  • 1520-5126
DOI
10.1021/jacs.0c11599
PMC
PMC8327756
OpenAlex
W3158817610
Cited by
183
References
4
Citations by year
2026: 29 · 2025: 33 · 2024: 30 · 2023: 42 · 2022: 34 · 2021: 14
FWCI
9.11
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of the American Chemical Society (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by-nc-nd)
  • mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion, other-oa)
  • Site cant be reached (submittedVersion, cc-by)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • PuSH - Publication Server of Helmholtz Zentrum München (submittedVersion, cc-by)
Funders
  • University of California
  • National Science Foundation (DGE-1144087)
  • Alfred P. Sloan Foundation (FG-2018-10855)
  • Deutsche Forschungsgemeinschaft (BR 5355/2-1)
  • U.S. Department of Health and Human Services (1R01EB027172)
  • U.S. Department of Energy (DE-SC0019245)
  • Helmholtz Pioneer Campus Institute for Biomedical Engineering
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
Keywords
polymethine dyes, short-wave infrared imaging, in vivo imaging, multiplexed imaging, indocyanine green, nonradiative decay, video-rate imaging, fluorescent probes
MeSH terms
Animals, Color, Fluorescent Dyes, Indoles, Infrared Rays, Molecular Structure, Mice, Optical Imaging

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