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Cited by 638

The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.

Gold Nanoparticles as a Vaccine Platform: Influence of Size and Shape on Immunological Responses in Vitro and in Vivo

Niikura, Kenichi, Matsunaga, Tatsuya, Suzuki, Tadaki, Kobayashi, Shintaro, Yamaguchi, Hiroki, Orba, Yasuko, Kawaguchi, Akira, Hasegawa, Hideki, Kajino, Kiichi, Ninomiya, Takafumi, Ijiro, Kuniharu, Sawa, Hirofumi·2013·ACS Nano
DOI 10.1021/nn3057005
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Abstract

This paper demonstrates how the shape and size of gold nanoparticles (AuNPs) affect immunological responses in vivo and in vitro for the production of antibodies for West Nile virus (WNV). We prepared spherical (20 and 40 nm in diameter), rod (40 × 10 nm), and cubic (40 × 40 × 40 nm) AuNPs as adjuvants and coated them with WNV envelope (E) protein. We measured anti-WNVE antibodies after inoculation of these WNVE-coated AuNPs (AuNP-Es) into mice. The 40 nm spherical AuNP-Es (Sphere40-Es) induced the highest level of WNVE-specific antibodies, while rod AuNP-Es (Rod-Es) induced only 50% of that of Sphere40-E. To examine the mechanisms of the shape-dependent WNVE antibody production, we next measured the efficiency of cellular uptake of AuNP-Es into RAW264.7 macrophage cells and bone-marrow-derived dendritic cells (BMDCs) and the subsequent cytokine secretion from BMDCs. The uptake of Rod-Es into the cells proceeded more efficiently than those of Sphere-Es or cubic WNVE-coated AuNPs (Cube-Es), suggesting that antibody production was not dependent on the uptake efficiency of the different AuNP-Es. Cytokine production from BMDCs treated with the AuNP-Es revealed that only Rod-E-treated cells produced significant levels of interleukin-1β (IL-1β) and interleukin-18 (IL-18), indicating that Rod-Es activated inflammasome-dependent cytokine secretion. Meanwhile, Sphere40-Es and Cube-Es both significantly induced inflammatory cytokine production, including tumor necrosis factor-α (TNF-α), IL-6, IL-12, and granulocyte macrophage colony-stimulating factor (GM-CSF). These results suggested that AuNPs are effective vaccine adjuvants and enhance the immune response via different cytokine pathways depending on their sizes and shapes.

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Metadata

Authors
Niikura, K., Matsunaga, T., Suzuki, T., Kobayashi, S., Yamaguchi, H., Orba, Y., Kawaguchi, A., Hasegawa, H., Kajino, K., Ninomiya, T., Ijiro, K., & Sawa, H.
Venue
ACS Nano
Publisher
American Chemical Society (ACS)
Published
2013-05-16
Volume
7
Issue
5
Pages
3926-3938
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
DOI
10.1021/nn3057005
OpenAlex
W1972187290
Cited by
638
References
10
FWCI
14.47
Type
journal article
Language
en
Open access
closed

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