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Fatty acid carbon is essential for dNTP synthesis in endothelial cells
Open accessAbstract
The metabolism of endothelial cells during vessel sprouting remains poorly studied. Here we report that endothelial loss of CPT1A, a rate-limiting enzyme of fatty acid oxidation (FAO), causes vascular sprouting defects due to impaired proliferation, not migration, of human and murine endothelial cells. Reduction of FAO in endothelial cells did not cause energy depletion or disturb redox homeostasis, but impaired de novo nucleotide synthesis for DNA replication. Isotope labelling studies in control endothelial cells showed that fatty acid carbons substantially replenished the Krebs cycle, and were incorporated into aspartate (a nucleotide precursor), uridine monophosphate (a precursor of pyrimidine nucleoside triphosphates) and DNA. CPT1A silencing reduced these processes and depleted endothelial cell stores of aspartate and deoxyribonucleoside triphosphates. Acetate (metabolized to acetyl-CoA, thereby substituting for the depleted FAO-derived acetyl-CoA) or a nucleoside mix rescued the phenotype of CPT1A-silenced endothelial cells. Finally, CPT1 blockade inhibited pathological ocular angiogenesis in mice, suggesting a novel strategy for blocking angiogenesis.
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“Acetyl CoA from FAO is used for the TCA cycle as well as for aspartate and nucleotide synthesis.52”
From The multifaceted contributions of mitochondria to cellular metabolism (2018) · cites this paper as [52]
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Jaffe, E. A., Nachman, R., Becker, C. G., & Minick, C. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107470 · Cited by 7,146
Basic and Therapeutic Aspects of Angiogenesis
Potente, M., Gerhardt, H., & Carmeliet, P. (2011) · Cell · doi:10.1016/j.cell.2011.08.039 · Cited by 2,771
Self-citationVisualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression
Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Osawa, H., Kashiwagi, S., Fukami, K., Miyata, T., Miyoshi, H., et al. (2008) · Cell · doi:10.1016/j.cell.2007.12.033 · Cited by 2,194
The transcription factor Myc controls metabolic reprogramming upon T lymphocyte activation
Wang, R., Dillon, C. P., Shi, L. Z., Milasta, S., Carter, R., Finkelstein, D., McCormick, L. L., Fitzgerald, P., Chi, H., Munger, J., et al. (2011) · Immunity · doi:10.1016/j.immuni.2011.09.021 · Cited by 2,140
Carmeliet, P., Moons, L., Luttun, A., Vincenti, V., Compernolle, V., Mol, M. D., Wu, Y., Bono, F., Devy, L., Beck, H., et al. (2001) · Nature Medicine · doi:10.1038/87904 · Cited by 1,708
Self-citationRole of PFKFB3-Driven Glycolysis in Vessel Sprouting
Bock, K. D., Γεωργιάδου, Μ., Schoors, S., Kuchnio, A., Wong, B. W., Cantelmo, A. R., Quaegebeur, A., Ghesquière, B., Cauwenberghs, S., Eelen, G., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.037 · Cited by 1,591
Self-citationCancer metabolism: fatty acid oxidation in the limelight
Carracedo, A., Cantley, L. C., & Pandolfi, P. P. (2013) · Nature reviews. Cancer · doi:10.1038/nrc3483 · Cited by 1,353
Ausprunk, D. H., & Folkman, J. (1977) · Microvascular Research · doi:10.1016/0026-2862(77)90141-8 · Cited by 1,290
The Notch Ligands Dll4 and Jagged1 Have Opposing Effects on Angiogenesis
Benedito, R., Roca, C., Sörensen, I., Adams, S., Gossler, A., Fruttiger, M., & Adams, R. H. (2009) · Cell · doi:10.1016/j.cell.2009.03.025 · Cited by 1,138
Recent molecular discoveries in angiogenesis and antiangiogenic therapies in cancer
Welti, J. C., Loges, S., Dimmeler, S., & Carmeliet, P. (2013) · Journal of Clinical Investigation · doi:10.1172/jci70212 · Cited by 601
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Helsley, R. N., Zelows, M. M., Noffsinger, V. P., Anspach, G., Dharanipragada, N., Mead, A. E., Cobo, I., Carter, A., Wu, Q., SHALAUROVA, I. Y., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.13.628437
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Zhang, Z., Qin, X., Liu, J., Yan, Z., Xie, H., & Zhang, M. (2025) · ACS Omega · doi:10.1021/acsomega.4c10288
Durot, S., Doubleday, P. F., Schulla, L., Sabine, A., Petrova, T. V., & Zamboni, N. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.03.03.641143
Liu, L., Yu, X., Chi, Z.-C., Shi, S., Lan, T., Leng, J.-H., Li, J.-H., He, Y., Qu, L.-Y., Jia, G.-L., et al. (2025) · SSRN Electronic Journal · doi:10.2139/ssrn.5249796
Sun, Q. L., Xie, L., An, H., Chen, W., Yang, Q., Wang, P., Tang, Y., & Peng, C. (2025) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2025.1585030
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Shen, Y. L., Lin, L., Yu, W., Cui, Z., Lin, T., Hu, S., Shi, Y., Gu, Y., Jin, M., & Lu, E. (2025) · International Dental Journal · doi:10.1016/j.identj.2025.100837
Hong, S., Shin, J., Saredy, J., Choi, S. Y., Wang, H., & Park, J. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.21.660867
Yuan, S. S., Hahn, S. A., Colussi, N., Mullett, S. J., Gelhaus, S. L., Schopfer, F. J., & Straub, A. C. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.17.676962
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Kaczara, P., Czyżyńska-Cichoń, I., Kuś, E., Kurpińska, A., Olkowicz, M., Wojnar‐Lason, K., Pacia, M. Z., Lytvynenko, O., Baes, M. I., & Chłopicki, S. (2024) · Figshare · doi:10.6084/m9.figshare.25792787
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Kaczara, P., Czyżyńska-Cichoń, I., Kuś, E., Kurpińska, A., Olkowicz, M., Wojnar‐Lason, K., Pacia, M. Z., Lytvynenko, O., Baes, M. I., & Chłopicki, S. (2024) · Figshare · doi:10.6084/m9.figshare.25792871
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Pain, E., Singh, P. K., Khan, Y., Mears, G., Yang, X., Lynham, S., Devlin, P. F., Rutherford, T., Jones, K. L., Walker, M. C., et al. (2026) · BMC Cancer · doi:10.1186/s12885-026-15909-3
Zheng, X., Ye, H., Zhang, T., Hu, X., Yang, R., Yu, L., & Yan, N. (2026) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.126.324346
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Fan, X., Zhou, R., Raftrey, B. C., Coronado, P. E. R., Trimm, E., Clancy, E., Chen, X., Bozeman, J., Chen, M. S., Alimukhamedov, S., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.04.29.721711
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Joo, J. H., Jidigam, V. K., & Rao, S. P. V. S. (2026) · Handbook of clinical neurology · doi:10.1016/b978-0-443-22193-4.00005-6
Warmke, N., Walker, A., & Cubbon, R. M. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-15734-9.00160-6
Riesner, K., Li, N., Jung, B., Fricke, C., Mandzimba‐Maloko, B., Mertlitz, S., Guevara, L. B., Amer, M., Jacobi, A., Kräter, M., et al. (2026) · Research Square · doi:10.21203/rs.3.rs-9620419/v1
Dreger, S. A., Kiu, R., & Robinson, S. D. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.05.29.728684
Citrin, K. M., Huang, Y., Ramos-Perez, A., Castellanos, C., Canfrán‐Duque, A., Zhang, X., Chaube, B., de Urturi, D. S., Gómez‐Coronado, D., Libreros, S., et al. (2026) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2026.120800
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Giorgio, F. D., Munaron, L., Tolosano, E., & Petrillo, S. (2026) · Cell Communication and Signaling · doi:10.1186/s12964-026-02986-x
Gómez-Escudero, J., Maniati, E., Holdsworth, J., Beattie, G., Hijazi, M., Guelbert, M., Elorbany, S., Cutillas, P. R., Wang, J., Hodivala‐Dilke, K., et al. (2026) · Angiogenesis · doi:10.1007/s10456-026-10063-7 · Cited by 1
Ye, K., Jiang, L., Chen, J., Yuan, S., Xiao, T., Chen, Y., Xiong, T., Luo, Z., Deng, C., & Xiao, S. (2026) · Frontiers in Physiology · doi:10.3389/fphys.2026.1865368
Shiino, S., Pascale, C. L., Motazedian, K., Lesser, E., Dumont, A., & Martinez, A. N. (2026) · Journal of Molecular Neuroscience · doi:10.1007/s12031-026-02576-z
The emerging metabolic role and treatment target of CPT1A in CRC
Zhu, X., Sun, T., Yuan, J., Mao, F., Jin, T., & Yi, C. (2026) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2026.1881073
Tang, D., Li, Q., Tacchi, C., Banchi, M., & Bocci, G. (2026) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2026.189713
Cao, X., Huang, S., Li, C., Jiang, S., 李俊如, Zhou, Y., Huang, K., Ma, W., Chu, Y., Yang, P., et al. (2026) · Environmental Science & Technology · doi:10.1021/acs.est.6c06569 · Cited by 1
Raval, K., Ilegems, E., Title, A. C., Fishburn, E., Bhardwaj, G., Mirguet, O., Ratcliffe, P. D., Greeff, O. B. W., van Wyk, A., & Tudzarova, S. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.09.20.752954
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Subramanian, A., Cuypers, A., Yagüe, P., Declercq, M., Souffreau, J., Coolen, P.-J., Sokol, L., Truong, A.-C., Borri, M., Veys, K., et al. (2026) · EMBO Molecular Medicine · doi:10.1038/s44321-026-00524-3
Self-citationGuo, X., Chao, A., Ding, Y., Yudan, 张. Z., Haijuan, 张. Z., 王金勇, Zhang, Y., & Wang, T. (2026) · Virulence · doi:10.1080/21505594.2026.2739013
Endothelial Dysfunction and Metabolic Reprogramming in Cardiovascular Diseases
Chen, H., Zhao, Y., Ge, C., Wang, J., Wang, Z., Wang, H., Cheng, S., Wang, Y., Jie, Y., Wang, D., et al. (2026) · Reviews in Cardiovascular Medicine · doi:10.31083/rcm51541
Wang, H., Li, Y., Qin, C., Chen, X., Wang, K., Zhang, M., & Cheng, W. (2026) · iScience · doi:10.1016/j.isci.2026.117553
Yang, J., Luo, Y., Zhou, Y., An, M., Pang, M., 李治华, Xie, Q., Zheng, H., Fang, Z., Kong, Y., et al. (2026) · Journal of Extracellular Vesicles · doi:10.1002/jev2.70383
Bunk, P., Ergin, I., Maher, T., Shah, V., Bonney, J. R., Kumar, L. B. S. S., Chung, C., Wellhausen, N., Moresco, P., Shao, S., et al. (2026) · Nature Immunology · doi:10.1038/s41590-026-02685-2
Mcintosh, O., Millan, M., Buch, A., Freissinet, C., Brucato, J., & Szopa, C. (2026) · Icarus · doi:10.1016/j.icarus.2026.117359
Liu, N., Zhou, D., Shi, M., Xiu, Y., Jiang, Y., Wang, Y., Moyano, G. A., McGuire, J. L., Iskender, A., Kilgore, M. D., et al. (2026) · Redox Biology · doi:10.1016/j.redox.2026.104446
Metadata
- Authors
- Schoors, S., Bruning, U., Missiaen, R., Queiroz, K. C. S., Borgers, G., Elia, I., Zecchin, A., Cantelmo, A. R., Christen, S., Goveia, J., Heggermont, W., Goddé, L., Vinckier, S., Van Veldhoven, P. P., Eelen, G., Schoonjans, L., Gerhardt, H., Dewerchin, M., Baes, M., De Bock, K., Ghesquière, B., Lunt, S. Y., Fendt, S.-M., & Carmeliet, P.
- Affiliations
- KU Leuven (BE)
- Universitair Ziekenhuis Leuven (BE)
- Max Delbrück Center (DE)
- Michigan State University (US)
- Corresponding author
- Fendt, Sarah-Maria
- Carmeliet, Peter
- ORCID
- Venue
- Nature
- Published
- 2015-04-01
- Volume
- 520
- Issue
- 7546
- Pages
- 192-197
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC4413024
- OpenAlex
- W2075209047
- Cited by
- 646
- References
- 37
- Citations by year
- 2026: 50 · 2025: 65 · 2024: 78 · 2023: 39 · 2022: 66 · 2021: 55 · 2020: 68 · 2019: 45 · 2018: 61 · 2017: 55 · 2016: 43 · 2015: 19
- FWCI
- 19.61
- Type
- journal article
- Language
- en
- Update notices
- correction (2015-08-05)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
- Lirias (KU Leuven) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- fatty acid oxidation, CPT1A, endothelial cells, angiogenesis, vessel sprouting, de novo nucleotide synthesis, dNTP synthesis, aspartic acid, uridine monophosphate, acetyl-CoA, TCA cycle, endothelial cell proliferation, pathological angiogenesis, retinopathy of prematurity, ocular angiogenesis
- MeSH terms
- Adenosine Triphosphate, Animals, Blood Vessels, Carbon, Carnitine O-Palmitoyltransferase, Citric Acid Cycle, Disease Models, Animal, DNA, Fatty Acids, Glucose, Humans, Neovascularization, Pathologic, Nucleotides, Oxidation-Reduction, Retinopathy of Prematurity, Acetic Acid, Gene Silencing, Endothelial Cells, Cell Line, Tumor, Cell Proliferation, Mice, Human Umbilical Vein Endothelial Cells
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