The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Efficient genome editing in zebrafish using a CRISPR-Cas system
Open accessAbstract
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
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“Also in January 2013, similar publications came out from the laboratories of Jennifer Doudna (Berkeley College, USA) [36], Jin-Soo Kim (Seoul University, South Korea) [37] and J. Keith Joung (Harvard School of Medicine, USA) [38].”
From CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing (2022) · cites this paper as [38]
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“Recently, the CRISPR/Cas system has been successfully applied to human cells19,20,21,22 and several model organisms for genome editing.23,24,25,26”
From The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo (2014) · cites this paper as [24]
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Citations
Cites
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Genome engineering using the CRISPR-Cas9 system
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013) · Nature Protocols · doi:10.1038/nprot.2013.143 · Cited by 11,133
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
Metadata
- Authors
- Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K.
- Affiliations
- Massachusetts General Hospital (US)
- Harvard University (US)
- Broad Institute (US)
- Corresponding author
- Yeh, J-R Joanna
- Joung, J Keith
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2013-01-29
- Volume
- 31
- Issue
- 3
- Pages
- 227-229
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC3686313
- OpenAlex
- W1976391205
- Cited by
- 2,937
- References
- 24
- Citations by year
- 2026: 68 · 2025: 125 · 2024: 146 · 2023: 159 · 2022: 207 · 2021: 203 · 2020: 227 · 2019: 230 · 2018: 252 · 2017: 246 · 2016: 306 · 2015: 276 · 2014: 300 · 2013: 189
- FWCI
- 160.03
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Helmholtz Centre for Ocean Research Kiel (GEOMAR) (acceptedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- National Institute on Aging (K01AG031300)
- National Human Genome Research Institute (P50HG005550)
- National Cancer Institute (R01CA140188)
- National Institute of General Medical Sciences (DP1GM105378, R01GM088040, F32GM105189)
- National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105378)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- CRISPR-Cas, genome editing, zebrafish embryos, Cas9 endonuclease, guide RNA, zinc finger nucleases, TALEN
- MeSH terms
- Animals, Base Sequence, DNA, Embryo, Nonmammalian, Endonucleases, Genetic Engineering, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Zebrafish, Genome, RNA Editing, DNA Cleavage, Inverted Repeat Sequences, RNA, Small Untranslated, CRISPR-Cas Systems
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