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Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
Open accessAbstract
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.
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Metadata
- Authors
- Gierahn, T. M., Wadsworth, M. H., Hughes, T. K., Bryson, B. D., Butler, A., Satija, R., Fortune, S., Love, J. C., & Shalek, A. K.
- Venue
- Nature Methods
- Published
- 2017-02-13
- Volume
- 14
- Issue
- 4
- Pages
- 395-398
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2588569583
- Cited by
- 865
- References
- 14
- FWCI
- 33.87
- Type
- journal article
- Language
- en
- Open access
- green
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