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A clinician's guide to microbiome analysis
Abstract
Microbiome analysis involves determining the composition and function of a community of microorganisms in a particular location. For the gastroenterologist, this technology opens up a rapidly evolving set of challenges and opportunities for generating novel insights into the health of patients on the basis of microbiota characterizations from intestinal, hepatic or extraintestinal samples. Alterations in gut microbiota composition correlate with intestinal and extraintestinal disease and, although only a few mechanisms are known, the microbiota are still an attractive target for developing biomarkers for disease detection and management as well as potential therapeutic applications. In this Review, we summarize the major decision points confronting new entrants to the field or for those designing new projects in microbiome research. We provide recommendations based on current technology options and our experience of sequencing platform choices. We also offer perspectives on future applications of microbiome research, which we hope convey the promise of this technology for clinical applications.
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“Rapid improvements in sequencing technology have facilitated the development of two techniques that are integral to answering these questions: 16S sequencing and whole genome shotgun sequencing (Claesson et al., 2017).”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Claesson et al., 2017
In the citing paper
“The large intestine is thus an important host site for microbial metabolism, a convenient truth that fortunately aligns with our reliance on the analysis of stool samples to gain insights into our microbial inhabitants (Fraher et al., 2012;Claesson et al., 2017).”
From Gut reactions: Breaking down xenobiotic–microbiome interactions (2019) · cites this paper as Claesson et al., 2017
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Metadata
- Authors
- Claesson, M. J., Clooney, A. G., & O'Toole, P. W.
- Affiliations
- University College Cork (IE)
- Corresponding author
- Claesson, Marcus J.
- Venue
- Nature Reviews Gastroenterology & Hepatology
- Published
- 2017-08-09
- Volume
- 14
- Issue
- 10
- Pages
- 585-595
- ISSN
- 1759-5045 (ISSN-L)
- 1759-5053
- OpenAlex
- W2743544008
- Cited by
- 153
- References
- 210
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 18 · 2023: 12 · 2022: 19 · 2021: 25 · 2020: 28 · 2019: 22 · 2018: 11 · 2017: 3
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Reviews Gastroenterology & Hepatology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Science Foundation Ireland
- APC Microbiome Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- microbiome analysis, gut microbiota, microbiota composition, microbiota function, biomarkers, microbiota-based therapy, intestinal diseases, extraintestinal diseases
- MeSH terms
- Gastrointestinal Microbiome, Genetic Techniques, Humans, Research Design, Computational Biology, Metagenomics
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