Combinatorial biosynthesis of reduced polyketides

Phylogenetic identification and in situ detection of individual microbial cells without cultivation

Amann, R., Ludwig, W., & SCHLEIFER, K. H. (1995) · Microbiological Reviews · doi:10.1128/mmbr.59.1.143-169.1995 · Cited by 9,093

Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)

Bentley, S. D., Chater, K. F., Cerdeño-Tárraga, A.-M., Challis, G. L., Thomson, N. R., James, K. D., Harris, D. E., Quail, M. A., Kieser, H., Harper, D., et al. (2002) · Nature · doi:10.1038/417141a · Cited by 3,233

Polyketide biosynthesis: a millennium review

Staunton, J., & Weissman, K. J. (2001) · Natural Product Reports · doi:10.1039/a909079g · Cited by 1,641

A new logic for DNA engineering using recombination in Escherichia coli

Zhang, Y., Buchholz, F., Muyrers, J. P. P., & Stewart, A. F. (1998) · Nature Genetics · doi:10.1038/2417 · Cited by 1,222

Modular Organization of Genes Required for Complex Polyketide Biosynthesis

Donadio, S., Staver, M. J., McAlpine, J. B., Swanson, S. J., & Katz, L. (1991) · Science · doi:10.1126/science.2024119 · Cited by 898

A new enzyme superfamily — the phosphopantetheinyl transferases

Lambalot, R. H., Gehring, A. M., Flugel, R. S., Zuber, P., LaCelle, M., Marahiel, M. A., Reid, R., Khosla, C., & Walsh, C. T. (1996) · Chemistry & Biology · doi:10.1016/s1074-5521(96)90181-7 · Cited by 851

Genome sequence of an industrial microorganism Streptomyces avermitilis : Deducing the ability of producing secondary metabolites

Ōmura, S., Ikeda, H., Ishikawa, J., Hanamoto, A., Takahashi, C., Shinose, M., Takahashi, Y., Horikawa, H., Nakazawa, H., Osonoe, T., et al. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.211433198 · Cited by 801

Where will new antibiotics come from?

Walsh, C. (2003) · Nature Reviews Microbiology · doi:10.1038/nrmicro727 · Cited by 755

Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli

Pfeifer, B. A., Admiraal, S. J., Gramajo, H., Cane, D. E., & Khosla, C. (2001) · Science · doi:10.1126/science.1058092 · Cited by 750

Characterization of Sfp, a Bacillus subtilis Phosphopantetheinyl Transferase for Peptidyl Carrier Protein Domains in Peptide Synthetases

Quadri, L. E. N., Weinreb, P. H., Lei, M., Nakano, M. M., Zuber, P., & Walsh, C. T. (1998) · Biochemistry · doi:10.1021/bi9719861 · Cited by 693

Structural and functional organization of the animal fatty acid synthase

Smith, S., Witkowski, A., & Joshi, A. K. (2003) · Progress in Lipid Research · doi:10.1016/s0163-7827(02)00067-x · Cited by 643

Polyketide and Nonribosomal Peptide Antibiotics: Modularity and Versatility

Walsh, C. T. (2004) · Science · doi:10.1126/science.1094318 · Cited by 628

An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea

Cortes, J., Haydock, S. F., Roberts, G. A., Bevitt, D. J., & Leadlay, P. F. (1990) · Nature · doi:10.1038/348176a0 · Cited by 624

A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles

Piel, J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.222481399 · Cited by 609

Harnessing the Biosynthetic Code: Combinations, Permutations, and Mutations

Cane, D. E., Walsh, C. T., & Khosla, C. (1998) · Science · doi:10.1126/science.282.5386.63 · Cited by 603

Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces species

Challis, G. L., & Hopwood, D. A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1934677100 · Cited by 597

Polyketide biosynthesis beyond the type I, II and III polyketide synthase paradigms

Shen, B. (2003) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(03)00020-6 · Cited by 565

Antitumor polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge Theonella swinhoei

Piel, J., Hui, D., Wen, G., Butzke, D., Platzer, M., Fusetani, N., & Matsunaga, S. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0405976101 · Cited by 543

Structure and Biosynthesis of the Jamaicamides, New Mixed Polyketide-Peptide Neurotoxins from the Marine Cyanobacterium Lyngbya majuscula

Edwards, D. J., Marquez, B. L., Nogle, L. M., McPhail, K., Goeger, D. E., Roberts, M. A., & Gerwick, W. H. (2004) · Chemistry & Biology · doi:10.1016/j.chembiol.2004.03.030 · Cited by 506

Engineered Biosynthesis of Novel Polyketides

McDaniel, R. N., Ebert‐Khosla, S., Hopwood, D. A., & Khosla, C. (1993) · Science · doi:10.1126/science.8248802 · Cited by 472

Natural products ? a simple model to explain chemical diversity

Firn, R. D., & Jones, C. G. (2003) · Natural Product Reports · doi:10.1039/b208815k · Cited by 467

Cloning and Heterologous Expression of the Epothilone Gene Cluster

Tang, L., Shah, S., Chung, L., Carney, J., Katz, L., Khosla, C., & Julien, B. (2000) · Science · doi:10.1126/science.287.5453.640 · Cited by 424

Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host

Malpartida, F., & Hopwood, D. A. (1984) · Nature · doi:10.1038/309462a0 · Cited by 401

New PCR Primers for the Screening of NRPS and PKS-I Systems in Actinomycetes: Detection and Distribution of These Biosynthetic Gene Sequences in Major Taxonomic Groups

Ayuso-Sacido, A., & Genilloud, O. (2005) · Microbial Ecology · doi:10.1007/s00248-004-0249-6 · Cited by 398

Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans

Stinear, T. P., Mve-Obiang, A., Small, P. L. C., Frigui, W., Pryor, M. J., Brosch, R., Jenkin, G. A., Johnson, P. D. R., Davies, J. K., Lee, R. E., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0305877101 · Cited by 389

Conserved Amino Acid Residues Correlating With Ketoreductase Stereospecificity in Modular Polyketide Synthases

Caffrey, P. (2003) · ChemBioChem · doi:10.1002/cbic.200300581 · Cited by 368

Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host. 1984.

Malpartida, F., & Hopwood, D. A. (1992) · PubMed · Cited by 360

Evolving strategies for enzyme engineering

BLOOM, J., MEYER, M., MEINHOLD, P., OTEY, C., MACMILLAN, D., & ARNOLD, F. (2005) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2005.06.004 · Cited by 355

Recombinant Environmental Libraries Provide Access to Microbial Diversity for Drug Discovery from Natural Products

Courtois, S., Cappellano, C. M., Ball, M., Francou, F.-X., Normand, P., Helynck, G., Martinez, A., Kolvek, S. J., Hopke, J., Osburne, M. S., et al. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.1.49-55.2003 · Cited by 350

Myxobacteria: proficient producers of novel natural products with various biological activities—past and future biotechnological aspects with the focus on the genus Sorangium

Gerth, K., Pradella, S., Perlova, O., Beyer, S., & Müller, R. (2003) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2003.07.015 · Cited by 330

Dissecting and Exploiting Intermodular Communication in Polyketide Synthases

Gokhale, R. S., Tsuji, S. Y., Cane, D. E., & Khosla, C. (1999) · Science · doi:10.1126/science.284.5413.482 · Cited by 326

Combinatorial polyketide biosynthesis by de novo design and rearrangement of modular polyketide synthase genes

Menzella, H. G., Reid, R., Carney, J. R., Chandran, S. S., Reisinger, S. J., Patel, K. G., Hopwood, D. A., & Santi, D. V. (2005) · Nature Biotechnology · doi:10.1038/nbt1128 · Cited by 323

A genomics-guided approach for discovering and expressing cryptic metabolic pathways

Zazopoulos, E., Huang, K., Staffa, A., Liu, W., Bachmann, B. O., Nonaka, K., Ahlert, J., Thorson, J. S., Shen, B., & Farnet, C. M. (2003) · Nature Biotechnology · doi:10.1038/nbt784 · Cited by 316

A Model of Structure and Catalysis for Ketoreductase Domains in Modular Polyketide Synthases

Reid, R., Piagentini, M., Rodriguez, E., Ashley, G., Viswanathan, N., Carney, J., Santi, D. V., Hutchinson, C. R., & McDaniel, R. (2003) · Biochemistry · doi:10.1021/bi0268706 · Cited by 310

The Calicheamicin Gene Cluster and Its Iterative Type I Enediyne PKS

Ahlert, J., Shepard, E., Lomovskaya, N., Zazopoulos, E., Staffa, A., Bachmann, B. O., Huang, K., Fonstein, L., Czisny, A., Whitwam, R. E., et al. (2002) · Science · doi:10.1126/science.1072105 · Cited by 292

The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum

Yu, T.-W., Bai, L., Clade, D., Hoffmann, D., Toelzer, S., Trinh, K. Q., Xu, J., Moss, S. J., Leistner, E., & Floss, H. G. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.092697199 · Cited by 286

Biosynthesis of the Enediyne Antitumor Antibiotic C-1027

Liu, W., Christenson, S. D., Standage, S., & Shen, B. (2002) · Science · doi:10.1126/science.1072110 · Cited by 281

λ Red-Mediated Genetic Manipulation of Antibiotic-Producing Streptomyces

Gust, B., Chandra, G., Jakimowicz, D., Yuqing, T., Bruton, C. J., & Chater, K. F. (2004) · Advances in Applied Microbiology · doi:10.1016/s0065-2164(04)54004-2 · Cited by 272

Modification of post-PKS tailoring steps through combinatorial biosynthesis

Rix, U., Fischer, C., Rix, L. L. R., & Rohr, J. (2002) · Natural Product Reports · doi:10.1039/b103920m · Cited by 266

Characterization of the Mupirocin Biosynthesis Gene Cluster from Pseudomonas fluorescens NCIMB 10586

El-Sayed, A. K., Hothersall, J., Cooper, S. M., Stephens, E., Simpson, T. J., & Thomas, C. M. (2003) · Chemistry & Biology · doi:10.1016/s1074-5521(03)00091-7 · Cited by 262

Biosynthesis of Polyketides in Heterologous Hosts

Pfeifer, B. A., & Khosla, C. (2001) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.65.1.106-118.2001 · Cited by 262

Benefit to Male Sailfin Mollies of Mating with Heterospecific Females

Schlupp, I., Marler, C., & Ryan, M. J. (1994) · Science · doi:10.1126/science.8278809 · Cited by 252

Repositioning of a Domain in a Modular Polyketide Synthase to Promote Specific Chain Cleavage

Cortés, J., Wiesmann, K. E. H., Roberts, G. A., Brown, M. J. B., Staunton, J., & Leadlay, P. F. (1995) · Science · doi:10.1126/science.7770773 · Cited by 251

Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl‐CoA:acyl carrier protein transacylase domains in modular polyketide synthases

Haydock, S. F., Aparicio, J. F., Molnár, I., Schwecke, T., Khaw, L. E., König, A., Marsden, A. F. A., Galloway, I. S., Staunton, J., & Leadlay, P. F. (1995) · FEBS Letters · doi:10.1016/0014-5793(95)01119-y · Cited by 243

Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster

Kodumal, S. J., Patel, K. G., Reid, R., Menzella, H. G., Welch, M., & Santi, D. V. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0406911101 · Cited by 235

Engineered Biosynthesis of a Complete Macrolactone in a Heterologous Host

Kao, C. M., Katz, L., & Khosla, C. (1994) · Science · doi:10.1126/science.8036492 · Cited by 229

Directed Evolution and Biocatalysis

Powell, K. A., Ramer, S. W., del Cardayré, S. B., Stemmer, W. P. C., Tobin, M. B., Longchamp, P. F., & Huisman, G. W. (2001) · Angewandte Chemie International Edition · doi:10.1002/1521-3773(20011105)40:21<3948::aid-anie3948>3.0.co;2-n · Cited by 226

The Structure of Docking Domains in Modular Polyketide Synthases

Broadhurst, R. W., Nietlispach, D., Wheatcroft, M. P., Leadlay, P. F., & Weissman, K. J. (2003) · Chemistry & Biology · doi:10.1016/s1074-5521(03)00156-x · Cited by 222

Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: Versatility from a unique substrate channel

Tsai, S.-C., Miercke, L. J. W., Krucinski, J., Gokhale, R., Chen, J. C.-H., Foster, P. G., Cane, D. E., Khosla, C., & Stroud, R. M. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.011399198 · Cited by 214

Alteration of the Substrate Specificity of a Modular Polyketide Synthase Acyltransferase Domain through Site-Specific Mutations

Reeves, C. D., Murli, S., Ashley, G. W., Piagentini, M., Hutchinson, C. R., & McDaniel, R. N. (2001) · Biochemistry · doi:10.1021/bi015864r · Cited by 192

A hybrid modular polyketide synthase obtained by domain swapping

Oliynyk, M., Brown, M. J. B., Cortés, J., Staunton, J., & Leadlay, P. F. (1996) · Chemistry & Biology · doi:10.1016/s1074-5521(96)90069-1 · Cited by 184

Iteration as Programmed Event during Polyketide Assembly; Molecular Analysis of the Aureothin Biosynthesis Gene Cluster

He, J., & Hertweck, C. (2003) · Chemistry & Biology · doi:10.1016/j.chembiol.2003.11.009 · Cited by 179

Merging the potential of microbial genetics with biological and chemical diversity: an even brighter future for marine natural product drug discovery

Salomon, C. E., Magarvey, N. A., & Sherman, D. H. (2004) · Natural Product Reports · doi:10.1039/b301384g · Cited by 171

Manipulation of macrolide ring size by directed mutagenesis of a modular polyketide synthase

Kao, C. M., Luo, G., Katz, L., Cane, D. E., & Khosla, C. (1995) · Journal of the American Chemical Society · doi:10.1021/ja00140a043 · Cited by 167

Stereospecific Acyl Transfers on the Erythromycin-Producing Polyketide Synthase

Marsden, A. F. A., Caffrey, P., Aparicio, J. F., Loughran, M. S., Staunton, J., & Leadlay, P. F. (1994) · Science · doi:10.1126/science.8278811 · Cited by 165

Phylogenetic Analysis of Polyketide Synthase I Domains from Soil Metagenomic Libraries Allows Selection of Promising Clones

Ginolhac, A., Jarrin, C., Gillet, B., Robe, P., Pujić, P., Tuphile, K., Bertrand, H., Vogel, T. M., Perrière, G., Simonet, P., et al. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.9.5522-5527.2004 · Cited by 159

Cloning and characterization of a gene cluster for geldanamycin production inStreptomyces hygroscopicusNRRL 3602

Rascher, A., Hu, Z., Viswanathan, N., Schirmer, A., Reid, R. C., Nierman, W. C., Lewis, M., & Hutchinson, C. R. (2003) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(02)01148-5 · Cited by 149

A multiplasmid approach to preparing large libraries of polyketides

Xue, Q., Ashley, G. W., Hutchinson, C. R., & Santi, D. V. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.21.11740 · Cited by 148

Engineered Biosynthesis of Geldanamycin Analogs for Hsp90 Inhibition

Patel, K. G., Piagentini, M., Rascher, A., Tian, Z., Buchanan, G. O., Regentin, R., Hu, Z., Hutchinson, C. R., & McDaniel, R. N. (2004) · Chemistry & Biology · doi:10.1016/j.chembiol.2004.09.012 · Cited by 131

Selective Protein−Protein Interactions Direct Channeling of Intermediates between Polyketide Synthase Modules

Tsuji, S. Y., Cane, D. E., & Khosla, C. (2001) · Biochemistry · doi:10.1021/bi002463n · Cited by 129

Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine

Milne, J. L. S., Shi, D., Rosenthal, P. B., Sunshine, J. S., Domingo, G. J., Wu, X., Brooks, B. R., Perham, R. N., Henderson, R., & Subramaniam, S. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf574 · Cited by 128

Biosynthesis of Yersiniabactin, a Complex Polyketide-Nonribosomal Peptide, Using Escherichia coli as a Heterologous Host

Pfeifer, B. A., Wang, C. C. C., Walsh, C. T., & Khosla, C. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.11.6698-6702.2003 · Cited by 126

Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae

Xue, Y., Wilson, D. J., Zhao, L., Liu, H., & Sherman, D. H. (1998) · Chemistry & Biology · doi:10.1016/s1074-5521(98)90293-9 · Cited by 125

Insights into Channel Architecture and Substrate Specificity from Crystal Structures of Two Macrocycle-Forming Thioesterases of Modular Polyketide Synthases,

Tsai, S., Lu, H., Cane, D. E., Khosla, C., & Stroud, R. M. (2002) · Biochemistry · doi:10.1021/bi0260177 · Cited by 124

Evidence for a double-helical structure for modular polyketide synthases

Staunton, J., Caffrey, P., Aparicio, J. F., Roberts, G. A., Saalau-Bethell, S. M., & Leadlay, P. F. (1996) · Nature Structural Biology · doi:10.1038/nsb0296-188 · Cited by 122

3-Amino-5-hydroxybenzoic Acid Synthase, the Terminal Enzyme in the Formation of the Precursor of mC7N Units in Rifamycin and Related Antibiotics

Kim, C.-G., Yu, T., Fryhle, C. B., Handa, S., & Floss, H. G. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.11.6030 · Cited by 111

Genetic Approaches to Polyketide Antibiotics. 1

McDaniel, R. N., Welch, M., & Hutchinson, C. R. (2005) · Chemical Reviews · doi:10.1021/cr0301189 · Cited by 111

Generation of polyketide libraries via combinatorial biosynthesis

Khosla, C., & Zawada, R. J. X. (1996) · Trends in biotechnology · doi:10.1016/0167-7799(96)10046-9 · Cited by 111

Generation of Multiple Bioactive Macrolides by Hybrid Modular Polyketide Synthases in Streptomyces venezuelae

Yoon, Y. J., Beck, B. J., Kim, B. S., Kang, H., Reynolds, K. A., & Sherman, D. H. (2002) · Chemistry & Biology · doi:10.1016/s1074-5521(02)00095-9 · Cited by 109

Polyketide Synthase Genes from Marine Dinoflagellates

Rein, K. S., Gibbs, P. D. L., Palacios, A., Abiy, L. W., DICKEY, R. W., Snyder, R. V., & Lopez, J. V. (2003) · Marine Biotechnology · doi:10.1007/s10126-002-0077-y · Cited by 106

Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: a route to simple statin analogues

Ranganathan, A., Timoney, M. C., Bycroft, M., Cortés, J., Thomas, I. P., Wilkinson, B., Kellenberger, L., Hanefeld, U., Galloway, I. S., Staunton, J., et al. (1999) · Chemistry & Biology · doi:10.1016/s1074-5521(00)80020-4 · Cited by 105

Production of the Potent Antibacterial Polyketide Erythromycin C in Escherichia coli

Peirú, S., Menzella, H. G., Rodrı́guez, E., Carney, J. R., & Gramajo, H. C. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.5.2539-2547.2005 · Cited by 103

Combinatorial biosynthesis of antimicrobials and other natural products

Rodríguez, E. (2001) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(00)00246-0 · Cited by 94

The Molecular Basis of Celmer's Rules: The Stereochemistry of the Condensation Step in Chain Extension on the Erythromycin Polyketide Synthase

Weissman, K. J., Timoney, M. C., Bycroft, M., Grice, P., Hanefeld, U., Staunton, J., & Leadlay, P. F. (1997) · Biochemistry · doi:10.1021/bi971566b · Cited by 94

Process and Metabolic Strategies for Improved Production of Escherichia coli -Derived 6-Deoxyerythronolide B

Pfeifer, B. A., Hu, Z., Licari, P., & Khosla, C. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.7.3287-3292.2002 · Cited by 93

Engineered biosynthesis of an ansamycin polyketide precursor in Escherichia coli

Watanabe, K., Rude, M. A., Walsh, C. T., & Khosla, C. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1632167100 · Cited by 89

Probing the Breadth of Macrolide Glycosyltransferases: In Vitro Remodeling of a Polyketide Antibiotic Creates Active Bacterial Uptake and Enhances Potency

Yang, M., Proctor, M. R., Bolam, D. N., Errey, J. C., Field, R. A., Gilbert, H. J., & Davis, B. G. (2005) · Journal of the American Chemical Society · doi:10.1021/ja051482n · Cited by 88

Biosynthesis of the anti‐parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea

Volchegursky, Y., Hu, Z., Katz, L., & McDaniel, R. N. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.02059.x · Cited by 88

Engineering specificity of starter unit selection by the erythromycin‐producing polyketide synthase

Long, P. F., Wilkinson, C. J., Bisang, C., Cortés, J., Dunster, N. J., Oliynyk, M., McCormick, E. L., McArthur, H. A. I., Méndez, C., Salas, J. A., et al. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02815.x · Cited by 88

Construction of desosamine containing polyketide libraries using a glycosyltransferase with broad substrate specificity

Tang, L., & McDaniel, R. N. (2001) · Chemistry & Biology · doi:10.1016/s1074-5521(01)00032-1 · Cited by 86

Engineering a polyketide with a longer chain by insertion of an extra module into the erythromycin-producing polyketide synthase

Rowe, C. J., Böhm, I. U., Thomas, I. P., et al. (2001) · Chemistry & Biology · doi:10.1016/s1074-5521(01)00024-2 · Cited by 84

Gain of Function Mutagenesis of the Erythromycin Polyketide Synthase. 2. Engineered Biosynthesis of an Eight-Membered Ring Tetraketide Lactone

Kao, C. M., McPherson, M., McDaniel, R. N., Fu, H. L., Cane, D. E., & Khosla, C. (1997) · Journal of the American Chemical Society · doi:10.1021/ja972609e · Cited by 78

Formation of functional heterologous complexes using subunits from the picromycin, erythromycin and oleandomycin polyketide synthases

Tang, L., Fu, H., & McDaniel, R. N. (2000) · Chemistry & Biology · doi:10.1016/s1074-5521(00)00073-9 · Cited by 77

Identification of a Phosphopantetheinyl Transferase for Erythromycin Biosynthesis in Saccharopolyspora erythraea

Weissman, K. J., Hong, H., Oliynyk, M., Siskos, A., & Leadlay, P. F. (2003) · ChemBioChem · doi:10.1002/cbic.200300775 · Cited by 75

Understanding Substrate Specificity of Polyketide Synthase Modules by Generating Hybrid Multimodular Synthases

Watanabe, K., Wang, C. C. C., Boddy, C. N. C., Cane, D. E., & Khosla, C. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305339200 · Cited by 75

Rapid engineering of polyketide overproduction by gene transfer to industrially optimized strains

Rodríguez, E., Hu, Z., Ou, S., Volchegursky, Y., Hutchinson, C. R., & McDaniel, R. N. (2003) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-003-0045-1 · Cited by 73

Cloning, Characterization and Heterologous Expression of a Polyketide Synthase and P-450 Oxidase Involved in the Biosynthesis of the Antibiotic Oleandomycin.

SHAH, S., XUE, Q., TANG, L., Carney, J. R., Betlach, M. C., & McDaniel, R. N. (2000) · The Journal of Antibiotics · doi:10.7164/antibiotics.53.502 · Cited by 68

Inactivation of the Carbamoyltransferase Gene Refines Post-Polyketide Synthase Modification Steps in the Biosynthesis of the Antitumor Agent Geldanamycin

Hong, Y., Lee, D., Kim, W.-C., Jeong, J.-K., Kim, C.-G., Sohng, J. K., Lee, J.-H., Paik, S., & Lee, J. J. (2004) · Journal of the American Chemical Society · doi:10.1021/ja047769m · Cited by 65

A novel actinomycete strain de-replication approach based on the diversity of polyketide synthase and nonribosomal peptide synthetase biosynthetic pathways

Ayuso‐Sacido, Á., Clark, D. J., González, I., Salazar, Ó., Anderson, A. S., & Genilloud, O. (2005) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-004-1828-7 · Cited by 64

Chemoenzymatic Route to Macrocyclic Hybrid Peptide/Polyketide-like Molecules

Kohli, R. M., Burke, M. D., Tao, J., & Walsh, C. T. (2003) · Journal of the American Chemical Society · doi:10.1021/ja0352202 · Cited by 62

Heterologous expression in Escherichia coli of an intact multienzyme component of the erythromycin‐producing polyketide synthase

Roberts, G. A., Staunton, J., & Leadlay, P. F. (1993) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1993.tb17925.x · Cited by 61

Precursor-Directed Biosynthesis

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