Immune-evasive human islet-like organoids ameliorate diabetes

STAR: ultrafast universal RNA-seq aligner

Dobin, A., Davis, C. A., Schlesinger, F., Drenkow, J., Zaleski, C., Jha, S., Batut, P., Chaisson, M., & Gingeras, T. R. (2013) · Bioinformatics · doi:10.1093/bioinformatics/bts635 · Cited by 51,709 · see citing works

Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities

Heinz, S., Benner, C., Spann, N., Bertolino, E., Lin, Y. C., Laslo, P., Cheng, J. X., Murre, C., Singh, H., & Glass, C. K. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.05.004 · Cited by 13,382 · see citing works

Integrating single-cell transcriptomic data across different conditions, technologies, and species

Butler, A., Hoffman, P., Smibert, P., Papalexi, E., & Satija, R. (2018) · Nature Biotechnology · doi:10.1038/nbt.4096 · Cited by 13,174 · see citing works

The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells

Trapnell, C., Cacchiarelli, D., Grimsby, J., Pokharel, P., Li, S., Morse, M., Lennon, N. J., Livak, K. J., Mikkelsen, T. S., & Rinn, J. L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2859 · Cited by 7,314 · see citing works

Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets.

Macosko, E. Z., Basu, A., Satija, R., Nemesh, J., Shekhar, K., Goldman, M., Tirosh, I., Bialas, A. R., Kamitaki, N., Martersteck, E. M., et al. (2015) · Cell · doi:10.1016/j.cell.2015.05.002 · Cited by 7,110 · see citing works

Differential analysis of gene regulation at transcript resolution with RNA-seq

Trapnell, C., Hendrickson, D. G., Sauvageau, M., Goff, L., Rinn, J. L., & Pachter, L. (2013) · Nature Biotechnology · doi:10.1038/nbt.2450 · Cited by 3,492 · see citing works

ATAC-seq: a method for assaying chromatin accessibility genome-wide

Buenrostro, J. D., Wu, B., Chang, H. Y., & Greenleaf, W. J. (2015) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb2129s109 · Cited by 3,289 · see citing works

Vascularized and functional human liver from an iPSC-derived organ bud transplant

Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170 · see citing works

Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling

Huang, S.-M. A., Mishina, Y., Liu, S.-M., Cheung, A. K., Stegmeier, F., Michaud, G., Charlat, O., Wiellette, E., Zhang, Y., Wiessner, S., et al. (2009) · Nature · doi:10.1038/nature08356 · Cited by 2,077 · see citing works

Generation of Functional Human Pancreatic β Cells In Vitro

Pagliuca, F. W., Millman, J. R., Gürtler, M., Segel, M., Van Dervort, A., Ryu, J. H., Peterson, Q. P., Greiner, D., & Melton, D. A. (2014) · Cell · doi:10.1016/j.cell.2014.09.040 · Cited by 2,025 · see citing works

Recovering Gene Interactions from Single-Cell Data Using Data Diffusion

van Dijk, D., Sharma, R., Nainys, J., Yim, K., Kathail, P., Carr, A. J., Burdziak, C., Moon, K. R., Chaffer, C. L., Pattabiraman, D., et al. (2018) · Cell · doi:10.1016/j.cell.2018.05.061 · Cited by 1,829 · see citing works

GOplot: an R package for visually combining expression data with functional analysis

Walter, W., Sánchez‐Cabo, F., & Ricote, M. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btv300 · Cited by 1,760 · see citing works

Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells

Rezania, A., Bruin, J. E., Arora, P., Rubin, A., Batushansky, I., Asadi, A., O'Dwyer, S., Quiskamp, N., Mojibian, M., Albrecht, T., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.3033 · Cited by 1,575 · see citing works

Tissue expression of PD-L1 mediates peripheral T cell tolerance

Keir, M., Liang, S. C., Guleria, I., Latchman, Y., Qipo, A., Albacker, L., Koulmanda, M., Freeman, G., Sayegh, M., & Sharpe, A. (2006) · Journal of Experimental Medicine · doi:10.1084/jem.20051776 · Cited by 1,264 · see citing works

Identification of novel transcripts in annotated genomes using RNA-Seq

Roberts, A., Pimentel, H., Trapnell, C., & Pachter, L. S. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr355 · Cited by 963 · see citing works

A choice of death - the signal-transduction of immune-mediated beta-cell apoptosis

Eizirik, D. L., & Mandrup-Poulsen, T. R. (2001) · Diabetologia · doi:10.1007/s001250100021 · Cited by 880 · see citing works

The Programmed Death-1 (PD-1) Pathway Regulates Autoimmune Diabetes in Nonobese Diabetic (NOD) Mice

Ansari, M. J., Salama, A. D., Chitnis, T., Smith, R. N., Yagita∥, H., Akiba, H., Yamazaki, T., Azuma, M., Iwai, H., Khoury, S. J., et al. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20022125 · Cited by 806 · see citing works

Controlled induction of human pancreatic progenitors produces functional beta‐like cells in vitro

Russ, H. A., Parent, A. V., Ringler, J. J., Hennings, T. G., Nair, G. G., Shveygert, M., Guo, T., Puri, S., Haataja, L., Cirulli, V., et al. (2015) · The EMBO Journal · doi:10.15252/embj.201591058 · Cited by 591 · see citing works

Metabolic Signaling in Fuel-Induced Insulin Secretion

Prentki, M., Matschinsky, F. M., & Madiraju, S. R. M. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2013.05.018 · Cited by 548 · see citing works

Recapitulating endocrine cell clustering in culture promotes maturation of human stem-cell-derived β cells

Nair, G. G., Liu, J. S., Russ, H. A., Tran, S., Saxton, M. S., Chen, R., Juang, C., Li, M.-L., Nguyen, V. Q., Giacometti, S., et al. (2019) · Nature Cell Biology · doi:10.1038/s41556-018-0271-4 · Cited by 472 · see citing works

Extracting Biological Meaning from Large Gene Lists with DAVID

Huang, D. W., Sherman, B. T., Zheng, X. M., Yang, J., Imamichi, T., Stephens, R. M., & Lempicki, R. A. (2009) · Current Protocols in Bioinformatics · doi:10.1002/0471250953.bi1311s27 · Cited by 457 · see citing works

Pancreas regeneration

Zhou, Q., & Melton, D. A. (2018) · Nature · doi:10.1038/s41586-018-0088-0 · Cited by 384 · see citing works

Functional β-cells maturation is marked by an increase in the glucose threshold for insulin secretion and by expression of urocortin3

Blum, B., Hrvatin, S., Schuetz, C., Bonal, C., Rezania, A., & Melton, D. (2012) · Nature Biotechnology · doi:10.1038/nbt.2141 · Cited by 349 · see citing works

Identification of proliferative and mature β-cells in the islets of Langerhans

Bader, E., Migliorini, A., Gegg, M., Moruzzi, N., Gerdes, J. M., Roscioni, S. S., Bakhti, M., Brandl, E., Irmler, M., Beckers, J., et al. (2016) · Nature · doi:10.1038/nature18624 · Cited by 338 · see citing works

Differentiated human stem cells resemble fetal, not adult, β cells

Hrvatin, S., O’Donnell, C. W., Deng, F., Millman, J. R., Pagliuca, F. W., DiIorio, P., Rezania, A., Gifford, D. K., & Melton, D. A. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1400709111 · Cited by 302 · see citing works

Urocortin3 mediates somatostatin-dependent negative feedback control of insulin secretion

van der Meulen, T., Donaldson, C. J., Cáceres, E., Hunter, A. E., Cowing-Zitron, C., Pound, L. D., Adams, M. W., Zembrzycki, A., Grove, K. L., & Huising, M. O. (2015) · Nature Medicine · doi:10.1038/nm.3872 · Cited by 256 · see citing works

Toward the development of a global induced pluripotent stem cell library

Turner, M., Leslie, S., Martin, N. G., Peschanski, M., Rao, M., Taylor, C. J., Trounson, A., Turner, D., Yamanaka, S., & Wilmut, I. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.08.003 · Cited by 245 · see citing works

MHC matching improves engraftment of iPSC-derived neurons in non-human primates

Morizane, A., Kikuchi, T., Hayashi, T., Mizuma, H., Takara, S., Doi, H., Mawatari, A., Glasser, M. F., Shiina, T., Ishigaki, H., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00926-5 · Cited by 244 · see citing works

Stem cell therapies for treating diabetes: progress and remaining challenges

Sneddon, J. B., Tang, Q., Stock, P., Bluestone, J., Roy, S., Desai, T., & Hebrok, M. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.05.016 · Cited by 222 · see citing works

β Cells that Resist Immunological Attack Develop during Progression of Autoimmune Diabetes in NOD Mice

Rui, J., Deng, S., Arazi, A., Perdigoto, A. L., Liu, Z. Z., & Herold, K. C. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.01.005 · Cited by 207 · see citing works

Vitamin D Switches BAF Complexes to Protect β Cells

Wei, Z., Yoshihara, E., He, N., Hah, N., Fan, W., Pinto, A. F. M., Huddy, T. F., Wang, Y., Ross, B., Estepa, G., et al. (2018) · Cell · doi:10.1016/j.cell.2018.04.013 · Cited by 206 · see citing works

PDL1 is expressed in the islets of people with type 1 diabetes and is up-regulated by interferons-α and-γ via IRF1 induction

Colli, M. L., Hill, J. L. E., Marroquí, L., Hopkinson, J. R., dos Santos, R. S., Leete, P., de Brachène, A. C., de Paula, F. M. M., Beeck, A. O. D., Castela, Â., et al. (2018) · EBioMedicine · doi:10.1016/j.ebiom.2018.09.040 · Cited by 193 · see citing works

ERRγ is required for the metabolic maturation of therapeutically functional glucose-responsive β cells

Yoshihara, E., Wei, Z., Lin, C.-S., Fang, S., Ahmadian, M., Kida, Y. S., Tseng, T. W., Dai, Y., Yu, R. T., Liddle, C., et al. (2016) · Cell Metabolism · doi:10.1016/j.cmet.2016.03.005 · Cited by 165 · see citing works

Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes

Osum, K. C., Burrack, A. L., Martinov, T., Sahli, N. L., Mitchell, J. S., Tucker, C., Pauken, K. E., Papas, K. K., Balamurugan, A. N., Spanier, J. A., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-26471-9 · Cited by 147 · see citing works

Paracrine signals regulate human liver organoid maturation from iPSC

Asai, A., Aihara, E., Watson, C. L., Mourya, R., Mizuochi, T., Shivakumar, P., Phelan, K. J., Mayhew, C. N., Helmrath, M. A., Takebe, T., et al. (2017) · Development · doi:10.1242/dev.142794 · Cited by 128 · see citing works

Disruption of TBP-2 ameliorates insulin sensitivity and secretion without affecting obesity

Yoshihara, E., Fujimoto, S., Inagaki, N., Okawa, K., Masaki, S., Yodoi, J., & Masutani, H. (2010) · Nature Communications · doi:10.1038/ncomms1127 · Cited by 117 · see citing works

TGFβ-dependent expression of PD-1 and PD-L1 controls CD8+ T cell anergy in transplant tolerance

Baas, M. C., Besançon, A., Goncalves, T., Valette, F., Yagita∥, H., Sawitzki, B., Volk, H., Waeckel-Énée, E., Rocha, B., Chatenoud, L., et al. (2016) · eLife · doi:10.7554/elife.08133 · Cited by 116 · see citing works

Urocortin 3 Marks Mature Human Primary and Embryonic Stem Cell-Derived Pancreatic Alpha and Beta Cells

van der Meulen, T., Xie, R., Kelly, O. G., Vale, W., Sander, M., & Huising, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0052181 · Cited by 98 · see citing works

Protective Role of Programmed Death 1 Ligand 1 (PD-L1)in Nonobese Diabetic Mice

Wang, C.-J., Chou, F., Chu, C.-H., Wu, J., Lin, S., Chang, D., & Sytwu, H. (2008) · Diabetes · doi:10.2337/db07-1260 · Cited by 88 · see citing works

All mixed up: defining roles for β-cell subtypes in mature isletsPreprint

Liu, J. S. E., & Hebrok, M. (2017) · Genes & Development · doi:10.1101/gad.294389.116 · Cited by 68 · see citing works

Closing in on Mass Production of Mature Human Beta Cells.

Kieffer, T. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.05.014 · Cited by 40 · see citing works

PD-1 pathway-mediated regulation of islet-specific CD4+ T cell subsets in autoimmune diabetes

Martinov, T., Spanier, J. A., Pauken, K. E., & Fife, B. T. (2016) · Immunoendocrinology · doi:10.14800/ie.1164 · Cited by 24 · see citing works

PD-L1 Deficiency within Islets Reduces Allograft Survival in Mice

Ma, D., Duan, W., Li, Y., Wang, Z., Li, S., Gong, N., Chen, G., Chen, Z., Wan, C., & Yang, J. (2016) · PLoS ONE · doi:10.1371/journal.pone.0152087 · Cited by 20 · see citing works

Organoids

Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,001 · see citing works

Engineering the next generation of cell-based therapeutics

Bashor, C. J., Hilton, I. B., Bandukwala, H. S., Smith, D. M., & Omid Veiseh, A. (2022) · Nature Reviews Drug Discovery · doi:10.1038/s41573-022-00476-6 · Cited by 448 · see citing works

Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells

Balboa, D., Barsby, T., Lithovius, V., Saarimäki-Vire, J., Omar-Hmeadi, M., Dyachok, O., Montaser, H., Lund, P.-E., Yang, M., Ibrahim, H., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01219-z · Cited by 433 · see citing works

Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids

Marsee, A., Roos, F. J. M., Verstegen, M. M. A., Marsee, A., Roos, F., Verstegen, M., Clevers, H., Vallier, L., Takebe, T., Huch, M., et al. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.04.005 · Cited by 310 · see citing works

Three-Dimensional Cell Cultures: The Bridge between In Vitro and In Vivo Models

Urzì, O., Gasparro, R., Costanzo, E., Luca, A. D., Giavaresi, G., Fontana, S., & Alessandro, R. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241512046 · Cited by 247 · see citing works

Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes

Hogrebe, N. J., Ishahak, M., & Millman, J. R. (2023) · Cell Stem Cell · doi:10.1016/j.stem.2023.04.002 · Cited by 210 · see citing works

Integrating organoids and organ-on-a-chip devices

Zhao, Y., Landau, S., Okhovatian, S., Liu, C., Lu, R. X. Z., Lai, B. F. L., Wu, Q., Kieda, J., Cheung, K., Rajasekar, S., et al. (2024) · Nature Reviews Bioengineering · doi:10.1038/s44222-024-00207-z · Cited by 206 · see citing works

Immunological considerations and challenges for regenerative cellular therapies

Petrus-Reurer, S., Romano, M., Howlett, S., Jones, J., Lombardi, G., & Saeb‐Parsy, K. (2021) · Communications Biology · doi:10.1038/s42003-021-02237-4 · Cited by 160 · see citing works

Single-cell transcriptomic and spatial landscapes of the developing human pancreas

Olaniru, O. E., Kadolsky, U. D., Kannambath, S., Vaikkinen, H., Fung, K., Dhami, P., & Persaud, S. J. (2022) · Cell Metabolism · doi:10.1016/j.cmet.2022.11.009 · Cited by 138 · see citing works

Organoid based personalized medicine: from bench to bedside

Li, Y., Tang, P., Cai, S., Peng, J., & Hua, G. (2020) · Cell Regeneration · doi:10.1186/s13619-020-00059-z · Cited by 138 · see citing works

Applications of iPSC-derived beta cells from patients with diabetes

Maxwell, K. G., & Millman, J. (2021) · Cell Reports Medicine · doi:10.1016/j.xcrm.2021.100238 · Cited by 118 · see citing works

Engineering islets from stem cells for advanced therapies of diabetes

Siehler, J., Blöchinger, A. K., Meier, M., & Lickert, H. (2021) · Nature Reviews Drug Discovery · doi:10.1038/s41573-021-00262-w · Cited by 125 · see citing works

Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes

Cayabyab, F., Nih, L. R., & Yoshihara, E. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.732431 · Cited by 119 · see citing works

Partners in Crime: Beta-Cells and Autoimmune Responses Complicit in Type 1 Diabetes Pathogenesis

Toren, E., Burnette, K., Banerjee, R. R., Hunter, C. S., & Tse, H. M. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.756548 · Cited by 110 · see citing works

Human hypoimmune primary pancreatic islets avoid rejection and autoimmunity and alleviate diabetes in allogeneic humanized mice

Hu, X., Gattis, C., Olroyd, A. G., Friera, A. M., White, K. A., Young, C., Basco, R., Lamba, M., Wells, F., Ankala, R., et al. (2023) · Science Translational Medicine · doi:10.1126/scitranslmed.adg5794 · Cited by 104 · see citing works

How the mechanical microenvironment of stem cell growth affects their differentiation: a review

Zhang, X., Zhang, S., & Wang, T. (2022) · Stem Cell Research & Therapy · doi:10.1186/s13287-022-03070-0 · Cited by 100 · see citing works

The promise of stem cell-derived islet replacement therapy

Melton, D. (2021) · Diabetologia · doi:10.1007/s00125-020-05367-2 · Cited by 96 · see citing works

TXNIP/TBP-2: A Master Regulator for Glucose Homeostasis

Yoshihara, E. (2020) · Antioxidants · doi:10.3390/antiox9080765 · Cited by 96 · see citing works

Selective deletion of human leukocyte antigens protects stem cell-derived islets from immune rejection

Parent, A. V., Faleo, G., Chavez, J., Saxton, M., Berrios, D. I., Kerper, N. R., Tang, Q., & Hebrok, M. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109538 · Cited by 96 · see citing works

Organoids: development and applications in disease models, drug discovery, precision medicine, and regenerative medicine

Yao, Q., Cheng, S., Pan, Q., Yu, J., Cao, G., Li, L., & Cao, H. (2024) · MedComm · doi:10.1002/mco2.735 · Cited by 97 · see citing works

Landscape of human organoids: Ideal model in clinics and research

Han, X., Cai, C., Deng, W., Shi, Y., Li, L., Wang, C., Zhang, J., Rong, M., Liu, J., Fang, B., et al. (2024) · The Innovation · doi:10.1016/j.xinn.2024.100620 · Cited by 98 · see citing works

Harnessing cellular therapeutics for type 1 diabetes mellitus: progress, challenges, and the road ahead

Grattoni, A., Korbutt, G., Tomei, A. A., García, A. J., Pepper, A. R., Stabler, C., Brehm, M., Papas, K., Citro, A., Shirwan, H., et al. (2025) · Nature Reviews Endocrinology · doi:10.1038/s41574-024-01029-0 · Cited by 92 · see citing works

Single-nucleus multi-omics of human stem cell-derived islets identifies deficiencies in lineage specification

Augsornworawat, P., Hogrebe, N. J., Ishahak, M., Schmidt, M. D., Marquez, E., Maestas, M. M., Veronese-Paniagua, D., Gale, S. E., Miller, J. R., Velazco-Cruz, L., et al. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01150-8 · Cited by 85 · see citing works

Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance

Gerace, D., Zhou, Q., Kenty, J. H.-R., Veres, A., Sintov, E., Wang, X., Boulanger, K. R., Li, H., & Melton, D. A. (2023) · Cell Reports Medicine · doi:10.1016/j.xcrm.2022.100879 · Cited by 88 · see citing works

Stem cells differentiation into insulin-producing cells (IPCs): recent advances and current challenges

Silva, I. B. B., Kimura, C. H., Colantoni, V. P., & Sogayar, M. (2022) · Stem cell research & therapeutics · doi:10.1186/s13287-022-02977-y · Cited by 68 · see citing works

Roles and action mechanisms of WNT4 in cell differentiation and human diseases: a review

Zhang, Q., Pan, Y., Ji, J., Xu, Y., Zhang, Q., & Qin, L. (2021) · Cell Death Discovery · doi:10.1038/s41420-021-00668-w · Cited by 84 · see citing works

The Human Islet: Mini-Organ With Mega-Impact

Walker, J. T., Saunders, D. C., Briššová, M., & Powers, A. C. (2021) · Endocrine Reviews · doi:10.1210/endrev/bnab010 · Cited by 80 · see citing works

Advances in Encapsulation and Delivery Strategies for Islet Transplantation

Wu, S., Wang, L., Fang, Y., Huang, H. W., You, X., & Wu, J. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202100965 · Cited by 78 · see citing works

The Wnt Signaling Pathway in Diabetic Nephropathy

Wang, H., Zhang, R., Wu, X., Chen, Y., Ji, W., Wang, J., Zhang, Y., Xia, Y., Tang, Y., & Yuan, J. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.701547 · Cited by 76 · see citing works

Deciphering early human pancreas development at the single-cell level

Ma, Z., Zhang, X., Zhong, W., Yi, H., Chen, X., Zhao, Y., Ma, Y., Song, E., & Xu, T. (2023) · Nature Communications · doi:10.1038/s41467-023-40893-8 · Cited by 73 · see citing works

Nerve Growth Factor-Laden Anisotropic Silk Nanofiber Hydrogels to Regulate Neuronal/Astroglial Differentiation for Scarless Spinal Cord Repair

Gao, X., Cheng, W., Zhang, X., Zhou, Z., Ding, Z., Zhou, X., Lü, Q., & Kaplan, D. L. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.1c19229 · Cited by 75 · see citing works

Towards a Functional Cure for Diabetes Using Stem Cell-Derived Beta Cells: Are We There Yet?

Bourgeois, S., Sawatani, T., Mulders, A. V., Leu, N. D., Heremans, Y., Heimberg, H., Cnop, M., & Staels, W. (2021) · Cells · doi:10.3390/cells10010191 · Cited by 61 · see citing works

Design Considerations for Macroencapsulation Devices for Stem Cell Derived Islets for the Treatment of Type 1 Diabetes

Goswami, D., Domingo‐Lopez, D. A., Ward, N. A., Millman, J. R., Duffy, G. P., Dolan, E. B., & Roche, E. T. (2021) · Advanced Science · doi:10.1002/advs.202100820 · Cited by 68 · see citing works

Maturation of beta cells: lessons from in vivo and in vitro models

Barsby, T., & Otonkoski, T. (2022) · Diabetologia · doi:10.1007/s00125-022-05672-y · Cited by 66 · see citing works

Protecting Stem Cell Derived Pancreatic Beta-Like Cells From Diabetogenic T Cell Recognition

Castro-Gutiérrez, R., Alkanani, A. K., Mathews, C. E., Michels, A. W., & Russ, H. A. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.707881 · Cited by 60 · see citing works

Human pluripotent stem cell-derived insulin-producing cells: A regenerative medicine perspective

Migliorini, A., Nostro, M. C., & Sneddon, J. B. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2021.03.021 · Cited by 61 · see citing works

The Importance of Proper Oxygenation in 3D Culture

Tse, H., Gardner, G., Domínguez-Bendala, J., & Fraker, C. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.634403 · Cited by 54 · see citing works

Engineering immune-evasive allogeneic cellular immunotherapies

Martin, K. E., Hammer, Q., Perica, K., Sadelain, M., & Malmberg, K. (2024) · Nature reviews. Immunology · doi:10.1038/s41577-024-01022-8 · Cited by 61 · see citing works

Generating 3D human cardiac constructs from pluripotent stem cells

Liu, C., Feng, X., Li, G., Gokulnath, P., & Xiao, J. (2022) · EBioMedicine · doi:10.1016/j.ebiom.2022.103813 · Cited by 57 · see citing works

Local Immunomodulatory Strategies to Prevent Allo‐Rejection in Transplantation of Insulin‐Producing Cells

Wang, X., Brown, N. K., Wang, B., Shariati, K., Wang, K., Fuchs, S., Melero‐Martin, J. M., & Ma, M. (2021) · Advanced Science · doi:10.1002/advs.202003708 · Cited by 57 · see citing works

Differential Function and Maturation of Human Stem Cell-Derived Islets After Transplantation

Maxwell, K. G., Kim, M. H., Gale, S. E., & Millman, J. R. (2022) · Stem Cells Translational Medicine · doi:10.1093/stcltm/szab013 · Cited by 54 · see citing works

Progress in Organ Bioprinting for Regenerative Medicine

Wang, X. S., Zhang, D., Singh, Y. P., Yeo, M., Deng, G., Lai, J., Chen, F., Ozbolat, I. T., & Yu, Y. (2024) · Engineering · doi:10.1016/j.eng.2024.04.023 · Cited by 59 · see citing works

CD81 marks immature and dedifferentiated pancreatic β-cells

Salinno, C., Büttner, M., Cota, P., Tritschler, S., Tarquis-Medina, M., Bastidas-Ponce, A., Scheibner, K., Burtscher, I., Böttcher, A., Theis, F. J., et al. (2021) · Molecular Metabolism · doi:10.1016/j.molmet.2021.101188 · Cited by 52 · see citing works

Transcriptional mechanisms of pancreatic β-cell maturation and functional adaptation

Wortham, M., & Sander, M. (2021) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2021.04.011 · Cited by 56 · see citing works

Human expandable pancreatic progenitor–derived β cells ameliorate diabetes

Ma, X., Lu, Y., Zhou, Z., Li, Q., Chen, X., Wang, W., Jin, Y., Hu, Z., Chen, G., Deng, Q., et al. (2022) · Science Advances · doi:10.1126/sciadv.abk1826 · Cited by 60 · see citing works

Islet organoid as a promising model for diabetes

Zhang, X., Ma, Z., Song, E., & Xu, T. (2021) · Protein & Cell · doi:10.1007/s13238-021-00831-0 · Cited by 52 · see citing works

Engineered immunomodulatory accessory cells improve experimental allogeneic islet transplantation without immunosuppression

Wang, X., Wang, K., Yu, M., Velluto, D., Hong, X., Wang, B., Chiu, A., Melero-Martin, J. M., Tomei, A. A., & Ma, M. (2022) · Science Advances · doi:10.1126/sciadv.abn0071 · Cited by 62 · see citing works

Emerging diabetes therapies: Bringing back the β-cells

Basile, G., Qadir, F., Mauvais-Jarvis, F., Vetere, A., Shoba, V., Modell, A. E., Pastori, R., Russ, H., Wagner, B., & Domínguez-Bendala, J. (2022) · Molecular Metabolism · doi:10.1016/j.molmet.2022.101477 · Cited by 47 · see citing works

Advancing Organoid Engineering for Tissue Regeneration and Biofunctional Reconstruction

Jin, H., Xue, Z., Liu, J., Ma, B., Yang, J., & Lei, L. (2024) · Biomaterials Research · doi:10.34133/bmr.0016 · Cited by 53 · see citing works

Stomach-derived human insulin-secreting organoids restore glucose homeostasis

Huang, X., Gu, W. Y., Zhang, J., Lan, Y., Colarusso, J. L., Li, S., Pertl, C., Lü, J., Kim, H., Zhu, J., et al. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01130-y · Cited by 54 · see citing works

From organoids to organoids-on-a-chip: Current applications and challenges in biomedical research

Liu, K., Chen, X., Fan, Z., Ren, F., Liu, J., & Hu, B. (2025) · Chinese Medical Journal · doi:10.1097/cm9.0000000000003535 · Cited by 56 · see citing works

Progress and challenges in developing allogeneic cell therapies

Deuse, T., & Schrepfer, S. (2025) · Cell Stem Cell · doi:10.1016/j.stem.2025.03.004 · Cited by 51 · see citing works

Organoids and regenerative hepatology

Jalan‐Sakrikar, N., Brevini, T., Huebert, R. C., & Sampaziotis, F. (2022) · Hepatology · doi:10.1002/hep.32583 · Cited by 50 · see citing works

Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression

Gravina, A., Tediashvili, G., Rajalingam, R., Quandt, Z., Deisenroth, C., Schrepfer, S., & Deuse, T. (2023) · Nature Biotechnology · doi:10.1038/s41587-022-01540-7 · Cited by 48 · see citing works

Current status of stem cell therapy for type 1 diabetes: a critique and a prospective consideration

Ghoneim, M. A., Gabr, M. M., El-Halawani, S. M., & Refaie, A. F. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03636-0 · Cited by 49 · see citing works

Complications and comorbidities associated with antineoplastic chemotherapy: Rethinking drug design and delivery for anticancer therapy

Mao, X., Wu, S., Huang, D., & Li, C. (2024) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2024.03.006 · Cited by 51 · see citing works

iPSC-Derived Organoids as Therapeutic Models in Regenerative Medicine and Oncology

Turhan, A. G., Hwang, J. W., Chaker, D., Tasteyre, A., Latsis, T., Griscelli, F., Desterke, C., & Griscelli, A. B. (2021) · Frontiers in Medicine · doi:10.3389/fmed.2021.728543 · Cited by 46 · see citing works

Emerging immunomodulatory strategies for cell therapeutics

Chua, C. Y. X., Jiang, A. Y., Eufrásio-da-Silva, T., Dolatshahi‐Pirouz, A., Langer, R., Orive, G., & Grattoni, A. (2022) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.11.008 · Cited by 46 · see citing works

Identification of unique cell type responses in pancreatic islets to stress

Maestas, M. M., Ishahak, M., Augsornworawat, P., Veronese-Paniagua, D., Maxwell, K. G., Velazco-Cruz, L., Marquez, E., Sun, J., Shunkarova, M., Gale, S. E., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49724-w · Cited by 51 · see citing works

Human Pluripotent Stem Cells to Model Islet Defects in Diabetes

Balboa, D., Iworima, D. G., & Kieffer, T. J. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.642152 · Cited by 47 · see citing works

Whole-genome CRISPR screening identifies genetic manipulations to reduce immune rejection of stem cell-derived islets

Sintov, E., Nikolskiy, I., Barrera, V., Hyoje-Ryu Kenty, J., Atkin, A. S., Gerace, D., Ho Sui, S. J., Boulanger, K., & Melton, D. A. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.08.002 · Cited by 45 · see citing works

In depth functional characterization of human induced pluripotent stem cell-derived beta cells in vitro and in vivo

Fantuzzi, F., Toivonen, S., Schiavo, A. A., Chae, H., Mohammad, T., Sawatani, T., Pachera, N., Cai, Y., Vinci, C., Virgilio, E., et al. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.967765 · Cited by 42 · see citing works

Advances in secondary prevention mechanisms of macrovascular complications in type 2 diabetes mellitus patients: a comprehensive review

Guan, H., Tian, J., Wang, Y., Niu, P., Zhang, Y., Zhang, Y., Fang, X., Miao, R., Yin, R., & Tong, X. (2024) · European journal of medical research · doi:10.1186/s40001-024-01739-1 · Cited by 42 · see citing works

Modeling human T1D-associated autoimmune processes

Maharlooei, M. K., Madley, R., Borsotti, C., Ferreira, L. M. R., Sharp, R. C., Brehm, M. A., Greiner, D. L., Parent, A. V., Anderson, M. S., Sykes, M., et al. (2021) · Molecular Metabolism · doi:10.1016/j.molmet.2021.101417 · Cited by 42 · see citing works

Making human pancreatic islet organoids: Progresses on the cell origins, biomaterials and three-dimensional technologies

Jiang, L., Shen, Y., Liu, Y., Zhang, L. J., & Jiang, W. (2022) · Theranostics · doi:10.7150/thno.66670 · Cited by 42 · see citing works

Genetically engineered hypoimmunogenic cell therapy

Hotta, A., Schrepfer, S., & Nagy, A. (2024) · Nature Reviews Bioengineering · doi:10.1038/s44222-024-00219-9 · Cited by 41 · see citing works

Immune Protection of Stem Cell-Derived Islet Cell Therapy for Treating Diabetes

Tahbaz, M., & Yoshihara, E. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.716625 · Cited by 42 · see citing works

Type 1 diabetes and engineering enhanced islet transplantation

Jeyagaran, A., Lu, C., Zbinden, A., Birkenfeld, A. L., Brucker, S. Y., & Layland, S. L. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114481 · Cited by 41 · see citing works

Proinflammatory signaling in islet β cells propagates invasion of pathogenic immune cells in autoimmune diabetes

Piñeros, A. R., Kulkarni, A., Gao, H., Orr, K. S., Glenn, L. M., Huang, F., Liu, Y., GANNON, M. A., Syed, F., Wu, W., et al. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.111011 · Cited by 38 · see citing works

Organoid-guided precision hepatology for metabolic liver disease

Osonoi, S., & Takebe, T. (2024) · Journal of Hepatology · doi:10.1016/j.jhep.2024.01.002 · Cited by 40 · see citing works

Immune Editing: Overcoming Immune Barriers in Stem Cell Transplantation

Meissner, T. B., Schulze, H. S., & Dale, S. M. (2022) · Current Stem Cell Reports · doi:10.1007/s40778-022-00221-0 · Cited by 39 · see citing works

NEUROD1 reinforces endocrine cell fate acquisition in pancreatic development

Bohuslavová, R., Fabriciova, V., Smolík, O., Lebrón‐Mora, L., Abaffy, P., Benesova, S., Žucha, D., Valihrach, L., Berková, Z., Saudek, F., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41306-6 · Cited by 39 · see citing works

A Century-long Journey From the Discovery of Insulin to the Implantation of Stem Cell–derived Islets

Ramzy, A., Belmonte, P. J., Braam, M. J. S., Ida, S., Wilts, E. M., Levings, M. K., Rezania, A., & Kieffer, T. J. (2022) · Endocrine Reviews · doi:10.1210/endrev/bnac021 · Cited by 36 · see citing works

Encapsulated islet transplantation

Liu, S. S., Shim, S., Kudo, Y., Stabler, C. L., O’Cearbhaill, E. D., Karp, J. M., & Yang, K. (2024) · Nature Reviews Bioengineering · doi:10.1038/s44222-024-00238-6 · Cited by 41 · see citing works

Genetic Engineering of Immune Evasive Stem Cell-Derived Islets

Sackett, S. D., Kaplan, S. J., Mitchell, S. A., Brown, M. E., Burrack, A. L., Grey, S., Huangfu, D., & Odorico, J. (2022) · Transplant International · doi:10.3389/ti.2022.10817 · Cited by 32 · see citing works

Pancreatic β-cell failure, clinical implications, and therapeutic strategies in type 2 diabetes

Cui, D., Feng, X., Lei, S., Zhang, H., Hu, W., Yang, S., Yu, X., & Su, Z. (2024) · Chinese Medical Journal · doi:10.1097/cm9.0000000000003034 · Cited by 36 · see citing works

Engineered vasculature induces functional maturation of pluripotent stem cell-derived islet organoids

Jun, Y., Nguyen-Ngoc, K.-V., Sai, S., Bender, R. H. F., Gong, W., Kravets, V. V., Zhu, H., Hatch, C. J., Schlichting, M., Gaetani, R., et al. (2022) · Developmental Cell · doi:10.1016/j.devcel.2025.04.024 · Cited by 43 · see citing works

Heterogeneity of Diabetes: β-Cells, Phenotypes, and Precision Medicine: Proceedings of an International Symposium of the Canadian Institutes of Health Research’s Institute of Nutrition, Metabolism and Diabetes and the U.S. National Institutes of Health’s National Institute of Diabetes and Digestive and Kidney Diseases

Cefalu, W. T., Andersen, D. K., Arreaza-Rubín, G., Pin, C. L., Sato, S. M., Verchere, C. B., Woo, M., & Rosenblum, N. D. (2021) · Diabetes Care · doi:10.2337/dci21-0051 · Cited by 36 · see citing works

From Disease and Patient Heterogeneity to Precision Medicine in Type 1 Diabetes

den Hollander, N. H. M., & Roep, B. O. (2022) · Frontiers in Medicine · doi:10.3389/fmed.2022.932086 · Cited by 32 · see citing works

Developing Hypoimmunogenic Human iPSC‐Derived Oligodendrocyte Progenitor Cells as an Off‐The‐Shelf Cell Therapy for Myelin Disorders

Feng, L., Chao, J.-F., Ye, P., Luong, Q., Sun, G.-Q., Liu, W., Cui, Q., Flores, S., Jackson, N., Shayento, A. N. H., et al. (2023) · Advancement of science · doi:10.1002/advs.202206910 · Cited by 32 · see citing works

An in situ dual-anchoring strategy for enhanced immobilization of PD-L1 to treat autoimmune diseases

Wang, S., Zhang, Y., Wang, Y., Yang, Y., Zhao, S., Sheng, T., Zhang, Y., Gu, Z., Wang, J., & Yu, J. (2023) · Nature Communications · doi:10.1038/s41467-023-42725-1 · Cited by 32 · see citing works

Islet Cell Replacement and Regeneration for Type 1 Diabetes: Current Developments and Future Prospects

Tondin, A. R., & Lanzoni, G. (2025) · BioDrugs · doi:10.1007/s40259-025-00703-7 · Cited by 32 · see citing works

Innovations in bio-engineering and cell-based approaches to address immunological challenges in islet transplantation

Ho, B. X., Teo, A. K. K., & Ng, N. H. J. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1375177 · Cited by 32 · see citing works

Enhanced viability and functional maturity of iPSC-derived islet organoids by collagen-VI-enriched ECM scaffolds.

Zhu, D., Chen, Z.-X., Guo, K., Xie, Q., Zou, Y., Mou, Q.-Z., Zhou, Z.-J., & Jin, G.-X. (2025) · Cell Stem Cell · doi:10.1016/j.stem.2025.02.001 · Cited by 30 · see citing works

From Mesenchymal Stromal/Stem Cells to Insulin-Producing Cells: Immunological Considerations

Refaie, A. F., Elbassiouny, B. L., Kloc, M., Sabek, O. M., Khater, S. M., Ismail, A. M., Mohamed, R. H., & Ghoneim, M. A. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.690623 · Cited by 31 · see citing works

Synthetic immune checkpoint engagers protect HLA-deficient iPSCs and derivatives from innate immune cell cytotoxicity

Gravina, A., Tediashvili, G., Zheng, Y., Iwabuchi, K. A., Peyrot, S. M., Roodsari, S. Z., Gargiulo, L., Kaneko, S., Osawa, M., Schrepfer, S., et al. (2023) · Cell stem cell · doi:10.1016/j.stem.2023.10.003 · Cited by 30 · see citing works

Bioengineered Artificial Extracellular Vesicles Presenting PD-L1 and Gal-9 Ameliorate New-Onset Type 1 Diabetes

Yang, Z., Zhang, Z., Li, L., Jing, Z., Ma, Y., Lan, T., Li, Y., Lin, Z., Fang, W., Zhang, J., et al. (2024) · Diabetes · doi:10.2337/db23-0987 · Cited by 30 · see citing works

Engineering human pluripotent stem cell lines to evade xenogeneic transplantation barriers

Pizzato, H. A., Alonso‐Guallart, P., Woods, J., Jóhannesson, B., Connelly, J. P., Fehniger, T. A., Atkinson, J. P., Pruett‐Miller, S. M., Monsma, F. J., & Bhattacharya, D. (2024) · Stem Cell Reports · doi:10.1016/j.stemcr.2023.12.003 · Cited by 28 · see citing works

Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets

Kim, M., Cho, S., Hwang, D. G., Shim, I., Kim, S. C., Jang, J., & Jang, J.-A. (2025) · Nature Communications · doi:10.1038/s41467-025-56665-5 · Cited by 31 · see citing works

Metabolic Studies in Organoids: Current Applications, Opportunities and Challenges

Richiardone, E., den Bossche, V. V., & Corbet, C. (2022) · Organoids · doi:10.3390/organoids1010008 · Cited by 29 · see citing works

Challenges of CRISPR/Cas-Based Cell Therapy for Type 1 Diabetes: How Not to Engineer a “Trojan Horse”

Karpov, D. S., Sosnovtseva, A. O., Pylina, S. V., Bastrich, A., Petrova, D. A., Kovalev, M. A., Shuvalova, A. I., Константиновна, Е. А., & Mokrysheva, N. G. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms242417320 · Cited by 29 · see citing works

Immuno-protective vesicle-crosslinked hydrogel for allogenic transplantation

Wang, Y., Huang, R., Lu, Y., Liu, M., & Mo, R. (2024) · Nature Communications · doi:10.1038/s41467-024-49135-x · Cited by 28 · see citing works

Islet Regeneration: Endogenous and Exogenous Approaches

Docherty, F. M., & Sussel, L. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073306 · Cited by 28 · see citing works

From Pancreatic β-Cell Gene Networks to Novel Therapies for Type 1 Diabetes

Eizirik, D. L., Szymczak, F., Alvelos, M. I., & Martin, F. (2021) · Diabetes · doi:10.2337/dbi20-0046 · Cited by 28 · see citing works

Overcoming the Limitations of Stem Cell-Derived Beta Cells

Karimova, M. V., Gvazava, I. G., & Vorotelyak, E. A. (2022) · Biomolecules · doi:10.3390/biom12060810 · Cited by 30 · see citing works

Three-in-one customized bioink for islet organoid: GelMA/ECM/PRP orchestrate pro-angiogenic and immunoregulatory function

Zhu, B., Wang, D., Pan, H., Gong, T., Ren, Q., Wang, Z., & Guo, Y. (2022) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2022.113017 · Cited by 28 · see citing works

Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives

Zhu, Z., Cheng, Y., Liu, X., Ding, W., Liu, J., Ling, Z., & Wu, L. (2025) · Cell Transplantation · doi:10.1177/09636897241303271 · Cited by 27 · see citing works

Organoid: Bridging the gap between basic research and clinical practice

Weng, G., Tao, J., Liu, Y., Qiu, J., Su, D., Wang, R., Luo, W., & Zhang, T. (2023) · Cancer Letters · doi:10.1016/j.canlet.2023.216353 · Cited by 27 · see citing works

Wnt4 is heterogeneously activated in maturing β-cells to control calcium signaling, metabolism and function

Katsumoto, K., Yennek, S., Chen, C., Delgadillo-Silva, L. F., Traikov, S., Sever, D., Azad, A., Shan, J., Vainio, S. J., Ninov, N., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-33841-5 · Cited by 25 · see citing works

Trends and challenges in organoid modeling and expansion with pluripotent stem cells and somatic tissue

Ge, J.-Y., Wang, Y., Li, Q., Liu, F.-K., Lei, Q.-K., & Zheng, Y. (2024) · PeerJ · doi:10.7717/peerj.18422 · Cited by 25 · see citing works

Type 1 diabetes mellitus: another step closer to pancreatic β-cell transplantation

Morris, A. W. J. (2020) · Nature Reviews Endocrinology · doi:10.1038/s41574-020-00417-6

Generation and long-term expansion of human pancreatic islet organoids in vitroPreprint

Song, W., Liu, C., Wang, S. Y., Wang, D., Xu, Y., Yuan, S., Chang, J., Zhang, B. Y., Xu, H. M., Fu, H., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.04.15.718643

Generation of hypoimmunogenic gastric insulin-secreting organoidsPreprint

Dattoli, A. A., Brown, M. E., Pearson, B., Feinstein, Z., Lan, Y., Polavarapu, V., Zhou, M., Nachman, R., Kelemen, Y., Rafii, S., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.07.08.736836

Immune Protection of Stem Cell-Derived Islet Cell Therapy for Treating Diabetes

Tahbaz, M., & Yoshihara, E. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.716625 · Cited by 42 · see citing works

Brown Adipose Tissue as a Unique Niche for Islet Organoid Transplantation: Insights From In Vivo Imaging

Sun, A., Hayat, H., Kenyon, E., Quadri, T., Amos, D., Perkins, K., Nigam, S., Tarleton, D., Mallett, C. L., Deng, C. X., et al. (2024) · Transplantation Direct · doi:10.1097/txd.0000000000001658 · Cited by 7 · see citing works