Cited by 284Single-cell and spatial transcriptomicsCell Image Analysis TechniquesGene expression and cancer classification

BANKSY unifies cell typing and tissue domain segmentation for scalable spatial omics data analysis

Open access
Singhal, Vipul, Chou, Nigel, Lee, Joseph, Yue, Yifei, Liu, Jinyue, Chock, Wan Kee, Lin, Li, Chang, Yun-Ching +5 more·2024·Nature Genetics

Published version of a preprint

Abstract

Spatial omics data are clustered to define both cell types and tissue domains. We present Building Aggregates with a Neighborhood Kernel and Spatial Yardstick (BANKSY), an algorithm that unifies these two spatial clustering problems by embedding cells in a product space of their own and the local neighborhood transcriptome, representing cell state and microenvironment, respectively. BANKSY’s spatial feature augmentation strategy improved performance on both tasks when tested on diverse RNA (imaging, sequencing) and protein (imaging) datasets. BANKSY revealed unexpected niche-dependent cell states in the mouse brain and outperformed competing methods on domain segmentation and cell typing benchmarks. BANKSY can also be used for quality control of spatial transcriptomics data and for spatially aware batch effect correction. Importantly, it is substantially faster and more scalable than existing methods, enabling the processing of millions of cell datasets. In summary, BANKSY provides an accurate, biologically motivated, scalable and versatile framework for analyzing spatially resolved omics data.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/s41588-024-01664-3

Cites

76 of 76 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Using BANKSY-derived spatial domains and Harmony correction, the study identified 18 reproducible niches shared across 20 samples.

    “Using BANKSY50-derived spatial domains and Harmony39 correction, we identified 18 reproducible niches shared across 20 samples (Figures S5A–S5C; STAR Methods).”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 77 references couldn’t be matched to a known paper.

Research works printed but not linked: 1

  1. [28]Huuki-Myers, L. et al. Integrated single cell and unsupervised spatial transcriptomic analysis defines molecular anatomy of the human dorsolateral prefrontal cortex. Preprint at bioRxiv10.1101/2023.02.15.528722 (2023).

Cited by

Sorted by citations

Authors
Singhal, V., Chou, N., Lee, J., Yue, Y., Liu, J., Chock, W. K., Lin, L., Chang, Y.-C., Teo, E. M. L., Aow, J., Lee, H. K., Chen, K. H., & Prabhakar, S.
Affiliations
  • Agency for Science, Technology and Research (SG)
  • National University of Singapore (SG)
Corresponding author
  • Chen, Kok Hao
  • Prabhakar, Shyam
Venue
Nature Genetics
Published
2024-02-27
Volume
56
Issue
3
Pages
431-441
ISSN
  • 1061-4036 (ISSN-L)
  • 1546-1718
OpenAlex
W4392198237
Cited by
282
References
77
Citations by year
2026: 124 · 2025: 103 · 2024: 47 · 2023: 3 · 2022: 5
FWCI
37.44
Type
journal article
Language
en
Open access
Locations
  • Nature Genetics (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DR-NTU (Nanyang Technological University) (submittedVersion, cc-by)
Funders
  • MOH | National Medical Research Council (OFIRG21jun-0090, OF-YIRG18nov-0014, OFIRG-000618-00)
  • Agency for Science, Technology and Research (#H18/01/a0/020, 202D800010, H18/01/a0/020, I1801E0029)
  • National Research Foundation Singapore (NRF-CRP25-2020-0001)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
Keywords
spatial omics, cell typing, spatial clustering, mouse brain, batch effect correction, scalable data analysis
MeSH terms
Data Analysis, Algorithms, Animals, RNA, Benchmarking, Gene Expression Profiling, Mice, Transcriptome

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review