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Cited by 89

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The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells

Askarian, Fatemeh, Ajayi, Clement, Hanssen, Anne-Merethe, van Sorge, Nina M., Pettersen, Ingvild, Diep, Dzung B., Sollid, Johanna U. E., Johannessen, Mona·2016·Scientific Reports
DOI 10.1038/srep22134
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Abstract

Staphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors involved in colonization are adhesins such as the microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Serine aspartate repeat containing protein D (SdrD) is involved in adhesion to human squamous cells isolated from the nose. Here, we identify Desmoglein 1 (Dsg1) as a novel interaction partner for SdrD. Genetic deletion of sdrD in S. aureus NCTC8325-4 through allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells in vitro. Complementary gain-of-function was demonstrated by heterologous expression of SdrD in Lactococcus lactis, which increased adherence to HaCaT cells. Also ectopic expression of Dsg1 in HEK293 cells resulted in increased adherence of S. aureus NCTC8325-4 in vitro. Increased adherence of NCTC8325-4, compared to NCTC8325-4Δ sdrD, to the recombinant immobilized Dsg1 demonstrated direct interaction between SdrD and Dsg1. Specificity of SdrD interaction with Dsg1 was further verified using flow cytometry and confirmed binding of recombinant SdrD to HaCaT cells expressing Dsg1 on their surface. These data demonstrate that Dsg1 is a host ligand for SdrD.

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Metadata

Authors
Askarian, F., Ajayi, C., Hanssen, A.-M., van Sorge, N. M., Pettersen, I., Diep, D. B., Sollid, J. U. E., & Johannessen, M.
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2016-02-29
Volume
6
Issue
1
ISSN
2045-2322
DOI
10.1038/srep22134
Cited by
89
References
6
Language
en
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CoChat. (2026). Citation check: The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells. https://app.cochat.ai/papers/10.1038/srep22134
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Askarian, F., Ajayi, C., Hanssen, A.-M., van Sorge, N. M., Pettersen, I., Diep, D. B., Sollid, J. U. E., & Johannessen, M. (2016). The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells. Scientific Reports. https://doi.org/10.1038/srep22134

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