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Cited by 1,375Hair Growth and DisordersSkin and Cellular Biology Researchmelanin and skin pigmentation

A Comprehensive Guide for the Accurate Classification of Murine Hair Follicles in Distinct Hair Cycle Stages

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Müller-Röver, Sven, Foitzik, Kerstin, Paus, Ralf, Handjiski, Bori, van der Veen, Carina, Eichmüller, Stefan, McKay, Ian A., Stenn, Kurt S.·2001·Journal of Investigative Dermatology
DOI 10.1046/j.0022-202x.2001.01377.x
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Abstract

Numerous strains of mice with defined mutations display pronounced abnormalities of hair follicle cycling, even in the absence of overt alterations of the skin and hair phenotype; however, in order to recognize even subtle, hair cycle-related abnormalities, it is critically important to be able to determine accurately and classify the major stages of the normal murine hair cycle. In this comprehensive guide, we present pragmatic basic and auxiliary criteria for recognizing key stages of hair follicle growth (anagen), regression (catagen) and quiescence (telogen) in C57BL/6NCrlBR mice, which are largely based on previous work from other authors. For each stage, a schematic drawing and representative micrographs are provided in order to illustrate these criteria. The basic criteria can be employed for all mouse strains and require only routine histochemical techniques. The auxiliary criteria depend on the immunohistochemical analysis of three markers (interleukin-1 receptor type I, transforming growth factor-beta receptor type II, and neural cell-adhesion molecule), which allow a refined analysis of anatomical hair follicle compartments during all hair cycle stages. In contrast to prior staging systems, we suggest dividing anagen III into three distinct substages, based on morphologic differences, onset and progression of melanogenesis, and the position of the dermal papilla in the subcutis. The computer-generated schematic representations of each stage are presented with the aim of standardizing reports on follicular gene and protein expression patterns. This guide should become a useful tool when screening new mouse mutants or mice treated with pharmaceuticals for discrete morphologic abnormalities of hair follicle cycling in a highly reproducible, easily applicable, and quantifiable manner.

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  • Follicles from i-TERT mice treated with doxycycline entered anagen by day 9 and were in mid-anagen by day 12, whereas non-transgenic follicles remained in telogen.

    “Whereas hair follicles from non-transgenic mice remained in telogen during the duration of the time course, follicles from i-TERT mice treated with doxycycline entered anagen by day 9 and were in mid-anagen20 by day 12 (Fig 2b) (chi-square analysis, p=0.005, Table S2).”

    From Conditional telomerase induction causes proliferation of hair follicle stem cells (2005) · cites this paper as [20]

  • Hair follicle cycles are synchronized for the first two postnatal periods of hair follicle growth, approximately 8 weeks.

    “exploiting the fact that hair follicle cycles are synchronized for the first two postnatal periods of hair follicle growth, approximately 8 weeks20.”

    From Conditional telomerase induction causes proliferation of hair follicle stem cells (2005) · cites this paper as [20]

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Authors
Müller-Röver, S., Foitzik, K., Paus, R., Handjiski, B., van der Veen, C., Eichmüller, S., McKay, I. A., & Stenn, K. S.
Affiliations
  • Universität Hamburg (DE)
  • Humboldt-Universität zu Berlin (DE)
  • Heidelberg University (DE)
  • University of London (GB)
  • Johnson & Johnson (United States) (US)
Corresponding author
Paus, Ralf
ORCID
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
2001-07
Volume
117
Issue
1
Pages
3-15
ISSN
0022-202X (ISSN-L)
DOI
10.1046/j.0022-202x.2001.01377.x
OpenAlex
W1964872204
Cited by
1,375
References
59
Citations by year
2026: 57 · 2025: 63 · 2024: 82 · 2023: 96 · 2022: 97 · 2021: 67 · 2020: 66 · 2019: 72 · 2018: 62 · 2017: 72 · 2016: 69 · 2015: 63 · 2014: 59 · 2013: 53 · 2012: 60
FWCI
4.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Investigative Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
Keywords
hair cycle, anagen, catagen, telogen, mouse mutants, IL1R1, CD56, melanogenesis, dermal papilla, hair follicle abnormalities
MeSH terms
Animals, Dermatology, Guidelines as Topic, Hair Follicle, Mice

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