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Cited by 129Fatty Acid Research and HealthEdible Oils Quality and AnalysisInflammatory mediators and NSAID effects

Pro- and anti-inflammatory cytokines in healthy volunteers fed various doses of fish oil for 1 year

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BLOK, W. L., DESLYPERE, J.‐P., DEMACKER, P. N. M., VAN DER VEN‐JONGEKRIJG, J., HECTORS, M. P. C., VAN DER MEER, J. W. M., KATAN, M. B.·1997·European Journal of Clinical Investigation
DOI 10.1046/j.1365-2362.1997.2240775.x
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Abstract

Dietary supplementation with n-3 fatty acids from fish oil alleviates inflammation in various chronic inflammatory disease states. Reductions in the production of pro-inflammatory cytokines interleukin 1 beta (IL-1 beta), tumour necrosis factor alpha (TNF-alpha), and interleukin 6 (IL-6) have been seen in humans after short-term n-3 fatty acid supplementation. We investigated long-term effects of dietary n-3 fatty acids on circulating cytokine concentrations and on ex vivo stimulated whole-blood production of IL-1 beta, TNF-alpha and interleukin 1 receptor antagonist (IL-1Ra), the naturally occurring antagonist of IL-1. A total of 58 monks with a mean age of 56 years were randomized into four groups and their diets were supplemented with 0, 3, 6, or 9 g of fish oil, providing 0, 1.06, 2.13 or 3.19 g of n-3 fatty acids per day. Subjects received equal amounts of saturated fatty acids, vitamin E and cholesterol. Compliance was excellent and erythrocyte fatty acid profiles closely reflected the amounts of n-3 fatty acids ingested. In the group receiving 9 g of fish oil per day, no influence of n-3 fatty acids on circulating cytokine concentrations was observed relative to placebo. Endotoxin-stimulated whole-blood cytokine production was measured at 26 and 52 weeks after the start and at 4, 8 and 26 weeks after cessation of supplementation. In all groups, the production of IL-1 beta and IL-1Ra was higher during supplementation than afterwards. However, no differences in cytokine production were noted between the placebo group and the various treatment groups at any point in time. Our results suggest that long-term supplementation of fish oil does not affect ex vivo cytokine production in man.

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  • Among subjects with low erythrocyte n-3 PUFA content, the 6054 G allele was associated with increased risk of metabolic syndrome (OR 3.29, 95% CI 1.49, 7.26 for GG and OR 1.95, 95% CI 0.85, 4.46 for GA) compared with AA genotype, but no significant genotype associations were found among subjects with high erythrocyte n-3 PUFA content.

    “Among subjects with low erythrocyte n-3 PUFA content (below the median), the 6054 G allele was associated with increased risk of the metabolic syndrome (OR 3•29, 95 % CI 1 •49, 7•26 for GG and OR 1•95, 95 % CI 0•85, 4•46 for GA) compared with the AA genotype, but there were no significant genotype associations among subjects with high erythrocyte n-3 PUFA content (above the median).”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [650]

  • Shen et al. identified that the IL-1b 6054 G>A SNP was significantly associated with CRP and adiponectin concentrations and with prevalence of metabolic syndrome among 1120 men and women with mean age 49.

    “They first identified that the IL-1b 6054 G .A SNP was significantly associated with CRP and adiponectin concentrations and with the prevalence of the metabolic syndrome among a group of 1120 men and women with a mean age of 49 years.”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [650]

  • There was a significant interaction between the IL-1b 6054 G>A polymorphism and erythrocyte membrane n-3 PUFA content.

    “There was also a significant interaction between this polymorphism and erythrocyte membrane n-3 PUFA content.”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [650]

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  1. 19 Endres S, Ghorbani R, Lonnemann G, van der Meer JWM, Dinarello CA. Measurement of immunoreactive interleukin-1 beta from human mononuclear cells: optimization of recovery, intrasub­ ject consistency, and comparison with interleukin -1 alpha and tumor necrosis factor. Clin Immunol Immunopathol 1988;49:424-38.

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Authors
BLOK, W. L., DESLYPERE, J. ‐., DEMACKER, P. N. M., VAN DER VEN‐JONGEKRIJG, J., HECTORS, M. P. C., VAN DER MEER, J. W. M., & KATAN, M. B.
Affiliations
  • Radboud University Nijmegen (NL)
  • Ghent University Hospital (BE)
  • Wageningen University & Research (NL)
Corresponding author
DEMACKER, P. N. M.
ORCID
KATAN, M. B.
Venue
European Journal of Clinical Investigation
Publisher
Wiley
Published
1997-12
Volume
27
Issue
12
Pages
1003-1008
ISSN
  • 0014-2972 (ISSN-L)
  • 1365-2362
DOI
10.1046/j.1365-2362.1997.2240775.x
OpenAlex
W2104625208
Cited by
129
References
35
Citations by year
2026: 1 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 4 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 1 · 2012: 7
FWCI
1.89
Type
journal article
Language
en
Open access
Licence
Locations
  • European Journal of Clinical Investigation (publishedVersion)
  • PubMed (publishedVersion)
  • VU Research Portal (publishedVersion, other-oa)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • Radboud Repository (Radboud University) (submittedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion)
  • Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam) (submittedVersion)
  • Radboud Repository (Radboud University) (submittedVersion, other-oa)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
  • Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
Keywords
fish oil, omega-3 fatty acids, dietary supplementation, inflammatory cytokines, anti-inflammatory cytokines, IL-1β, TNF-α, interleukin-6, interleukin-1 receptor antagonist, whole blood cytokine production, endotoxin stimulation, erythrocyte fatty acids, long-term supplementation, healthy volunteers
MeSH terms
Adult, Aged, Aged, 80 and over, Fish Oils, Humans, Inflammation, Interleukin-1, Male, Middle Aged, Patient Compliance, Sialoglycoproteins, Tumor Necrosis Factor-alpha, Cytokines, Dietary Supplements, Interleukin 1 Receptor Antagonist Protein

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