Cited by 7Thyroid Disorders and TreatmentsMetabolomics and Mass Spectrometry StudiesAnalytical chemistry methods development

Mass Spectrometry-Based Determination of Thyroid Hormones and Their Metabolites in Endocrine Diagnostics and Biomedical Research – Implications for Human Serum Diagnostics

Köhrle, Josef, Richards, Keith H.·2020·Experimental and Clinical Endocrinology & Diabetes

Abstract

The wide spectrum of novel applications for the LC-MS/MS-based analysis of thyroid hormone metabolites (THM) in blood samples and other biological specimen highlights the perspectives of this novel technology. However, thorough development of pre-analytical sample workup and careful validation of both pre-analytics and LC-MS/MS analytics, is needed, to allow for quantitative detection of the thyronome, which spans a broad concentration range in these biological samples. This minireview summarizes recent developments in advancing LC-MS/MS-based analytics and measurement of total concentrations of THM in blood specimen of humans, methods in part further refined in the context of previous achievements analyzing samples derived from cell-culture or tissues. Challenges and solutions to tackle efficient pre-analytic sample extraction and elimination of matrix interferences are compared. Options for automatization of pre-analytic sample-preparation and comprehensive coverage of the wide thyronome concentration range are presented. Conventional immunoassay versus LC-MS/MS-based determination of total and free THM concentrations are briefly compared.

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Crossref · 10.1055/a-1175-4610

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Authors
Köhrle, J., & Richards, K. H.
Affiliations
Humboldt-Universität zu Berlin (DE)
Corresponding author
Köhrle, Josef
Venue
Experimental and Clinical Endocrinology & Diabetes
Published
2020-06
Volume
128
Issue
06/07
Pages
358-374
ISSN
  • 0947-7349 (ISSN-L)
  • 1439-3646
OpenAlex
W3034824079
Cited by
7
References
84
Citations by year
2026: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2020: 2
FWCI
0.15
Type
journal article
Language
en
Open access
closed
Locations
  • Experimental and Clinical Endocrinology & Diabetes (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
Keywords
thyroid hormones, thyroid hormone metabolites, LC-MS/MS, human serum, matrix effects, sample extraction, assay validation, free thyroid hormones, immunoassay comparison, endocrine diagnostics
MeSH terms
Chromatography, Liquid, Humans, Thyroid Function Tests, Thyroid Hormones, Biomedical Research, Tandem Mass Spectrometry

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