Mass Spectrometry-Based Determination of Thyroid Hormones and Their Metabolites in Endocrine Diagnostics and Biomedical Research – Implications for Human Serum Diagnostics
Abstract
The wide spectrum of novel applications for the LC-MS/MS-based analysis of thyroid hormone metabolites (THM) in blood samples and other biological specimen highlights the perspectives of this novel technology. However, thorough development of pre-analytical sample workup and careful validation of both pre-analytics and LC-MS/MS analytics, is needed, to allow for quantitative detection of the thyronome, which spans a broad concentration range in these biological samples. This minireview summarizes recent developments in advancing LC-MS/MS-based analytics and measurement of total concentrations of THM in blood specimen of humans, methods in part further refined in the context of previous achievements analyzing samples derived from cell-culture or tissues. Challenges and solutions to tackle efficient pre-analytic sample extraction and elimination of matrix interferences are compared. Options for automatization of pre-analytic sample-preparation and comprehensive coverage of the wide thyronome concentration range are presented. Conventional immunoassay versus LC-MS/MS-based determination of total and free THM concentrations are briefly compared.
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- Authors
- Köhrle, J., & Richards, K. H.
- Affiliations
- Humboldt-Universität zu Berlin (DE)
- Corresponding author
- Köhrle, Josef
- Venue
- Experimental and Clinical Endocrinology & Diabetes
- Publisher
- Georg Thieme Verlag KG
- Published
- 2020-06
- Volume
- 128
- Issue
- 06/07
- Pages
- 358-374
- ISSN
- 0947-7349 (ISSN-L)
- 1439-3646
- OpenAlex
- W3034824079
- Cited by
- 7
- References
- 84
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2020: 2
- FWCI
- 0.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental and Clinical Endocrinology & Diabetes (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
- Keywords
- thyroid hormones, thyroid hormone metabolites, LC-MS/MS, human serum, matrix effects, sample extraction, assay validation, free thyroid hormones, immunoassay comparison, endocrine diagnostics
- MeSH terms
- Chromatography, Liquid, Humans, Thyroid Function Tests, Thyroid Hormones, Biomedical Research, Tandem Mass Spectrometry
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