Cloning and Characterization of HuR, a Ubiquitously Expressed Elav-like Protein
Open accessAbstract
The neuronal-specific Elav-like proteins (HuD, Hel-N, and HuC) contain three RNP-type concensus motifs and bind to AU-rich elements. We have identified and cloned a fourth member of this family (HuR) that is expressed in a wide variety of cell types. The purified recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs. In the case of the c-fos AU-rich element, HuR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Mutational analysis has shown that all three AU motifs are required for maximal binding.
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ELAVL proteins recognize U- and AU-rich elements.
“that recognize U- and AU-rich elements112–118”
From The neurogenetics of alternative splicing (2016) · cites this paper as [113]
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- Authors
- Ma, W.-J., Cheng, S., Campbell, C., Wright, A., & Furneaux, H.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Cleveland Clinic (US)
- Corresponding author
- Furneaux, Henry
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-04
- Volume
- 271
- Issue
- 14
- Pages
- 8144-8151
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2060708740
- Cited by
- 667
- References
- 34
- Citations by year
- 2026: 9 · 2025: 15 · 2024: 13 · 2023: 17 · 2022: 19 · 2021: 17 · 2020: 19 · 2019: 17 · 2018: 16 · 2017: 10 · 2016: 17 · 2015: 24 · 2014: 26 · 2013: 27 · 2012: 21
- FWCI
- 8.68
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns029682-04)
- National Cancer Institute (P30CA008748)
- National Institute of Neurological Disorders and Stroke (NS29682)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- HuR, ELAV-like proteins, AU-rich elements, AUUUA motif, c-fos mRNA, RNA-binding proteins, protein-DNA binding specificity
- MeSH terms
- ELAV-Like Protein 1, Amino Acid Sequence, Antigens, Surface, Base Sequence, Binding Sites, Cloning, Molecular, Gene Expression Regulation, Humans, Interleukin-3, Molecular Sequence Data, RNA, Messenger, Genes, myc, Sequence Alignment, RNA-Binding Proteins, Genes, fos, Sequence Homology, Amino Acid, DNA Primers, DNA, Complementary, ELAV Proteins
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