C/EBP is an immediate-early gene required for the consolidation of long-term facilitation in Aplysia
Abstract
The consolidation of long-term memory requires protein and mRNA synthesis. A similar requirement has been demonstrated for learning-related synaptic plasticity in the gill-withdrawal reflex of Aplysia. The monosynaptic component of this reflex can be reconstituted in vitro, where it undergoes both short- and long-term increases in synaptic strength in response to serotonin (5-HT), a neurotransmitter released during behavioral sensitization, a simple form of learning. As with sensitization, the long-term synaptic modification is characterized by a brief consolidation period during which gene expression is required. We find that during this phase, the transcription factor Aplysia CCAAT enhancer-binding protein (ApC/EBP) is induced rapidly by 5-HT and by cAMP, even in the presence of protein synthesis inhibitors. Blocking the function of ApC/EBP blocks long-term facilitation selectively without affecting the short-term process. These data indicate that cAMP-inducible immediate-early genes have an essential role in the consolidation of stable long-term synaptic plasticity in Aplysia.
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The induction and consolidation of LTF in vitro depends on the activity of several transcription factors.
“The induction and consolidation of LTF in vitro depends on the activity of several transcription factors24–27.”
From Computational design of enhanced learning protocols (2012) · cites this paper as [24]
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Deficit in Long-Term Synaptic Plasticity Is Rescued by a Computationally Predicted Stimulus Protocol
Liu, R.-Y., Zhang, Y., Baxter, D. A., Smolen, P., Cleary, L. J., & Byrne, J. H. (2013) · The Journal of Neuroscience · doi:10.1523/jneurosci.0643-13.2013 · Cited by 29 · see citing works
Characterization and phosphorylation of CREB-like proteins in Aplysia central nervous system
Dash, P. K., & Moore, A. N. (1996) · Molecular Brain Research · doi:10.1016/0169-328x(95)00350-2 · Cited by 27 · see citing works
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Self-citationZhou, L., Zhang, Y., Liu, R.-Y., Smolen, P., Cleary, L. J., & Byrne, J. H. (2015) · The Journal of Neuroscience · doi:10.1523/jneurosci.3330-14.2015 · Cited by 14 · see citing works
Lee, Y.-S., Choi, S.-L., Jun, H., Yim, S.-J., Lee, J.-A., Kim, H. F., Lee, S.-H., Shim, J., Lee, K., Jang, D.-J., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1116224109 · Cited by 15 · see citing works
- Authors
- Alberini, C. M., Ghirardl, M., Metz, R., & Kandel, E. R.
- Affiliations
- Howard Hughes Medical Institute (US)
- Bristol-Myers Squibb (United States) (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1994-03
- Volume
- 76
- Issue
- 6
- Pages
- 1099-1114
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2055829187
- Cited by
- 523
- References
- 78
- Citations by year
- 2025: 4 · 2024: 6 · 2023: 4 · 2022: 9 · 2021: 8 · 2020: 7 · 2019: 7 · 2018: 6 · 2017: 14 · 2016: 8 · 2015: 13 · 2014: 13 · 2013: 17 · 2012: 16
- FWCI
- 14.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- C/EBP, immediate early genes, long-term memory consolidation, gill-withdrawal reflex, Aplysia, serotonin, cAMP signaling, synaptic plasticity, behavioral sensitization, transcription factors, protein synthesis
- MeSH terms
- Amino Acid Sequence, Animals, Aplysia, Avian Sarcoma Viruses, Base Sequence, Cells, Cultured, Cloning, Molecular, DNA-Binding Proteins, Memory, Molecular Sequence Data, Neuronal Plasticity, Nuclear Proteins, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Serotonin, Transcription Factors, Sequence Homology, Amino Acid, Genes, Immediate-Early, CCAAT-Enhancer-Binding Proteins
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