CoChat
Sign Up
Cited by 309

The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.

Palaeoproteomic evidence identifies archaic hominins associated with the Châtelperronian at the Grotte du Renne

Welker, Frido, Hajdinjak, Mateja, Talamo, Sahra, Jaouen, Klervia, Dannemann, Michael, David, Francine, Julien, Michèle, Meyer, Matthias, Kelso, Janet, Barnes, Ian, Brace, Selina, Kamminga, Pepijn, Fischer, Roman, Kessler, Benedikt M., Stewart, John R., Pääbo, Svante, Collins, Matthew J., Hublin, Jean-Jacques·2016·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1605834113
Open this paper in CoChat — free account

Abstract

Significance: The displacement of Neandertals by anatomically modern humans (AMHs) 50,000–40,000 y ago in Europe has considerable biological and behavioral implications. The Châtelperronian at the Grotte du Renne (France) takes a central role in models explaining the transition, but the association of hominin fossils at this site with the Châtelperronian is debated. Here we identify additional hominin specimens at the site through proteomic zooarchaeology by mass spectrometry screening and obtain molecular (ancient DNA, ancient proteins) and chronometric data to demonstrate that these represent Neandertals that date to the Châtelperronian. The identification of an amino acid sequence specific to a clade within the genus Homo demonstrates the potential of palaeoproteomic analysis in the study of hominin taxonomy in the Late Pleistocene and warrants further exploration.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.1605834113

Cites

17 of 17 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Distinct SAPs in the bone protein COL10a1 enabled the identification of Neanderthal remains using paleoproteomics.

    “This approach was adopted in the identification of Neanderthal remains using paleoproteomics, through the identification of distinct SAPs in the bone protein COL10a1”

    From Ancient protein analysis in archaeology (2021) · cites this paper as [145]

    The Palaeoproteomic (LC-MS/MS) analysis reports one COL10α1 amino-acid position indicative of an archaic sequence, supporting the role of a protein variant in identifying the remains. It does not report multiple distinct COL10α1 substitutions, and that position is shared with Denisovans; the Discussion attributes Neandertal ancestry to the biomolecular evidence together, including mitochondrial evidence. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 48

  1. RE Green, , A draft sequence of the Neandertal genome. Science 328, 710–722 (2010). · DOI · Paper page
  2. SE Churchill, FH Smith, Makers of the early Aurignacian of Europe. Am J Phys Anthropol 31, 61–115 (2000). · DOI
  3. M Roussel, M Soressi, J-J Hublin, The Châtelperronian conundrum: Blade and bladelet lithic technologies from Quinçay, France. J Hum Evol 95, 13–32 (2016). · DOI
  4. J-M Granger F Lévêque Parure castelperronienne et aurignacienne: Étude de trois séries inédites de dents percées et comparaisons. CR Acad Sci Ser IIa Sci Terre Planets 325(7):537–543. (1997). · DOI
  5. F d’Errico, M Julien, D Liolios, M Vanhaeren, D Baffier, Many awls in our argument. Bone tool manufacture and use in the Châtelperronian and Aurignacian levels of the Grotte du Renne at Arcy-sur-Cur. The Chronology of the Aurignacian and of the Transitional Technocomplexes. Dating, Stratigraphies, Cultural Implications, Trabalhos de arquelogia, eds J Zilhão, F d’Errico (Instituto Português de Arqueologia, Lisboa), pp. 247–270 (2003).
  6. J-J Hublin, F Spoor, M Braun, F Zonneveld, S Condemi, A late Neanderthal associated with Upper Palaeolithic artefacts. Nature 381, 224–226 (1996). · DOI
  7. SE Bailey, J-J Hublin, Dental remains from the Grotte du Renne at Arcy-sur-Cure (Yonne). J Hum Evol 50, 485–508 (2006). · DOI
  8. SE Bailey, TD Weaver, J-J Hublin, Who made the Aurignacian and other early Upper Paleolithic industries? J Hum Evol 57, 11–26 (2009). · DOI
  9. J-J Hublin, The modern human colonization of western Eurasia: When and where? Quat Sci Rev 118, 194–210 (2015). · DOI
  10. T Higham, , Chronology of the Grotte du Renne (France) and implications for the context of ornaments and human remains within the Châtelperronian. Proc Natl Acad Sci USA 107, 20234–20239 (2010). · DOI
  11. R White, Personal ornaments from the Grotte du Renne at Arcy-sur-Cure. Athena Review 2, 41–46 (2001).
  12. O Bar-Yosef, J-G Bordes, Who were the makers of the Châtelperronian culture? J Hum Evol 59, 586–593 (2010). · DOI
  13. J-J Hublin, , Radiocarbon dates from the Grotte du Renne and Saint-Césaire support a Neandertal origin for the Châtelperronian. Proc Natl Acad Sci USA 109, 18743–18748 (2012). · DOI
  14. F Caron, F d’Errico, P Del Moral, F Santos, J Zilhão, The reality of Neandertal symbolic behavior at the Grotte du Renne, Arcy-sur-Cure, France. PLoS One 6, e21545 (2011). · DOI
  15. M Buckley, M Collins, J Thomas-Oates, JC Wilson, Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 23, 3843–3854 (2009). · DOI · Paper page
  16. F Welker, M Soressi, W Rendu, J-J Hublin, MJ Collins, Using ZooMS to identify fragmentary bone from the Late Middle/Early Upper Palaeolithic sequence of Les Cottés, France. J Archaeol Sci 54, 279–286 (2015). · DOI · Paper page
  17. S Brown, , Identification of a new hominin bone from Denisova Cave, Siberia using collagen fingerprinting and mitochondrial DNA analysis. Sci Rep 6, 23559 (2016). · DOI · Paper page
  18. CM Nielsen-Marsh, , Osteocalcin protein sequences of Neanderthals and modern primates. Proc Natl Acad Sci USA 102, 4409–4413 (2005). · DOI
  19. F Welker, , Ancient proteins resolve the evolutionary history of Darwin’s South American ungulates. Nature 522, 81–84 (2015). · DOI · Paper page
  20. NL van Doorn, H Hollund, MJ Collins, A novel and non-destructive approach for ZooMS analysis: Ammonium bicarbonate buffer extraction. Archaeol Anthropol Sci 3, 281–289 (2011). · DOI · Paper page
  21. M Buckley, SW Kansa, Collagen fingerprinting of archaeological bone and teeth remains from Domuztepe, South Eastern Turkey. Archaeol Anthropol Sci 3, 271–280 (2011). · DOI
  22. S Evans, , Using combined biomolecular methods to explore whale exploitation and social aggregation in hunter–gatherer–fisher society in Tierra del Fuego. J Archaeol Sci: Reports 6, 757–767 (2015).
  23. NE Robinson, , Structure-dependent nonenzymatic deamidation of glutaminyl and asparaginyl pentapeptides. J Pept Res 63, 426–436 (2004). · DOI
  24. NL van Doorn, J Wilson, H Hollund, M Soressi, MJ Collins, Site-specific deamidation of glutamine: A new marker of bone collagen deterioration. Rapid Commun Mass Spectrom 26, 2319–2327 (2012). · DOI · Paper page
  25. E Cappellini, , Proteomic analysis of a pleistocene mammoth femur reveals more than one hundred ancient bone proteins. J Proteome Res 11, 917–926 (2012). · DOI
  26. C Wadsworth, M Buckley, Proteome degradation in fossils: Investigating the longevity of protein survival in ancient bone. Rapid Commun Mass Spectrom 28, 605–615 (2014). · DOI
  27. R Hjorten, , Type XXVII collagen at the transition of cartilage to bone during skeletogenesis. Bone 41, 535–542 (2007). · DOI
  28. F Long, Building strong bones: Molecular regulation of the osteoblast lineage. Nat Rev Mol Cell Biol 13, 27–38 (2011). · DOI
  29. N Matsuo, , Collagen XXIV (Col24a1) gene expression is a specific marker of osteoblast differentiation and bone formation. Connect Tissue Res 49, 68–75 (2008). · DOI
  30. TF Linsenmayer, RD Eavey, TM Schmid, Type X collagen: A hypertrophic cartilage-specific molecule. Pathol Immunopathol Res 7, 14–19 (1988). · DOI
  31. AK Coussens, , Unravelling the molecular control of calvarial suture fusion in children with craniosynostosis. BMC Genomics 8, 458 (2007). · DOI
  32. S Castellano, , Patterns of coding variation in the complete exomes of three Neandertals. Proc Natl Acad Sci USA 111, 6666–6671 (2014). · DOI · Paper page
  33. C Warinner, , Pathogens and host immunity in the ancient human oral cavity. Nat Genet 46, 336–344 (2014). · DOI · Paper page
  34. R Kendall, J Hendy, MJ Collins, AR Millard, RL Gowland, Poor preservation of antibodies in archaeological human bone and dentine. STAR: Science & Technology of Archaeological Research 2, 15–24 (2016). · DOI · Paper page
  35. NE Robinson, A Robinson Molecular Clocks: Deamidation of Asparaginyl and Glutaminyl Residues in Peptides and Proteins (Althouse Press, Cave Junction, OR, 2004).
  36. A Kong, , A high-resolution recombination map of the human genome. Nat Genet 31, 241–247 (2002). · DOI
  37. MP Richards, S Mays, BT Fuller, Stable carbon and nitrogen isotope values of bone and teeth reflect weaning age at the Medieval Wharram Percy site, Yorkshire, UK. Am J Phys Anthropol 119, 205–210 (2002). · DOI
  38. E Discamps, B Gravina, N Teyssandier, In the eye of the beholder: Contextual issues for Bayesian modelling at the Middle-to-Upper Palaeolithic transition. World Archaeol 47, 601–621 (2015). · DOI
  39. T Higham, , The timing and spatiotemporal patterning of Neanderthal disappearance. Nature 512, 306–309 (2014). · DOI
  40. P Bodu, L’application de la méthode des remontages à l’étude du matériel lithique des premiers niveaux châtelperroniens d’Arcy-sur-Cure. Paléolithique Moyen Récent et Paléolithique Supérieur Ancien En Europe. Ruptures et Transitions: Examen Critique Des Documents Archéologiques, ed C Farzy (APRAIF, Nemours), pp. 309–312 (1990).
  41. HL Movius, The Châtelperronian in French Archaeology: The Evidence of Arcy-sur-Cure. Antiquity 43, 111–123 (1969). · DOI
  42. J Zilhão, F d’Errico, M Julien, F David, Chronology of the site of Grotte du Renne, Arcy-sur-Cure, France: Implications for radiocarbon dating. Before Farming 2011, 1–14 (2011). · DOI
  43. K Ruebens, SJP McPherron, J-J Hublin, On the local Mousterian origin of the Châtelperronian: Integrating typo-technological, chronostratigraphic and contextual data. J Hum Evol 86, 55–91 (2015). · DOI
  44. REM Hedges, JG Clement, CDL Thomas, TC O’connell, Collagen turnover in the adult femoral mid-shaft: Modeled from anthropogenic radiocarbon tracer measurements. Am J Phys Anthropol 133, 808–816 (2007). · DOI
  45. L Orlando, , Recalibrating Equus evolution using the genome sequence of an early Middle Pleistocene horse. Nature 499, 74–78 (2013). · DOI · Paper page
  46. M-T Gansauge, M Meyer, Selective enrichment of damaged DNA molecules for ancient genome sequencing. Genome Res 24, 1543–1549 (2014). · DOI · Paper page
  47. P Korlević, , Reducing microbial and human contamination in DNA extractions from ancient bones and teeth. Biotechniques 59, 87–93 (2015). · DOI · Paper page
  48. S Talamo, M Richards, A comparison of bone pretreatment methods for AMS dating of samples> 30,000 BP. Radiocarbon 53, 443–449 (2011). · DOI

Cited by

Sorted by citations

Metadata

Authors
Welker, F., Hajdinjak, M., Talamo, S., Jaouen, K., Dannemann, M., David, F., Julien, M., Meyer, M., Kelso, J., Barnes, I., Brace, S., Kamminga, P., Fischer, R., Kessler, B. M., Stewart, J. R., Pääbo, S., Collins, M. J., & Hublin, J.-J.
Affiliations
  • Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany;
  • Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany;
  • Paris Unité Mixte de Recherche 7041, Archéologies et Sciences de l'Antiquité, Centre National de la Recherche Scientifique, 92023 Nanterre, France;
  • Department of Earth Sciences, Natural History Museum, London SW7 5BD, United Kingdom;
  • Naturalis Biodiversity Center, 2300 RA Leiden, The Netherlands;
  • Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7FZ, United Kingdom;
  • Faculty of Science and Technology, Bournemouth University, Dorset BH12 5BB, United Kingdom
  • BioArCh, University of York, York YO10 5DD, United Kingdom;
ORCID
Kelso, Janet
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2016-09-16
Volume
113
Issue
40
Pages
11162-11167
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.1605834113
Cited by
309
References
17
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review