Barcoded nanoparticles for high throughput in vivo discovery of targeted therapeutics
Open accessAbstract
Significance: The effectiveness of nucleic acid drugs is limited by inefficient delivery to target tissues and cells and by unwanted accumulation in off-target organs. Although thousands of chemically distinct nanoparticles can be synthesized, nanoparticles designed to deliver nucleic acids in vivo were first tested in cell culture, yielding poor predictions for delivery in vivo. To facilitate testing of many nanoparticles in vivo, we designed and optimized a high-throughput DNA barcoding system to simultaneously measure nucleic acid delivery mediated by dozens of distinct nanoparticles in a single mouse. This nano-barcoding system can be used to study hundreds, or even thousands, of nanoparticles directly in vivo and could dramatically accelerate the discovery and understanding of nanoparticle drug delivery systems.
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Dahlman et al. designed and optimized a high-throughput DNA barcoding system for detection of lipid nanoparticles carrying specific nucleic acid barcodes in normal or tumor tissues in vivo, facilitating discovery of nanoparticles targeting tumor tissues and cells.
“Dahlman et al. designed and optimized a high-throughput DNA barcoding system for the detection of lipid nanoparticles carrying specific nucleic acid barcodes in normal or tumor tissues in vivo, which facilitates the discovery of nanoparticles targeting tumor tissues and cells.339”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [339]
Barcoded nanoparticles allow in vivo high-throughput profiling of lipid nanoparticle distribution at the cell level.
“However, barcoded nanoparticles allow in vivo high-throughput profiling of lipid nanoparticle distribution at the cell level121.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [121]
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Principles of nanoparticle design for overcoming biological barriers to drug delivery
Blanco, E., Shen, H., & Ferrari, M. (2015) · Nature Biotechnology · doi:10.1038/nbt.3330 · Cited by 7,002 · see citing works
Development and Applications of CRISPR-Cas9 for Genome Engineering
Hsu, P. D., Lander, E. S., & Zhang, F. (2014) · Cell · doi:10.1016/j.cell.2014.05.010 · Cited by 5,762 · see citing works
Next-generation DNA sequencing
Shendure, J., & Ji, H. (2008) · Nature Biotechnology · doi:10.1038/nbt1486 · Cited by 4,509 · see citing works
Knocking down barriers: advances in siRNA delivery
Whitehead, K. A., Langer, R., & Anderson, D. G. (2009) · Nature Reviews Drug Discovery · doi:10.1038/nrd2742 · Cited by 3,115 · see citing works
Self-citationBiomolecular coronas provide the biological identity of nanosized materials
Monopoli, M. P., Åberg, C., Salvati, A., & Dawson, K. A. (2012) · Nature Nanotechnology · doi:10.1038/nnano.2012.207 · Cited by 3,017 · see citing works
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
Platt, R. J., Chen, S., Zhou, Y., Yim, M. J., Swiech, L., Kempton, H. R., Dahlman, J. E., Parnas, O., Eisenhaure, T. M., Jovanovic, M., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.014 · Cited by 2,001 · see citing works
Self-citationRational design of cationic lipids for siRNA delivery
Semple, S. C., Akinc, A., Chen, J., Sandhu, A. P., Mui, B. L., Cho, C. K., Sah, D. W. Y., Stebbing, D., Crosley, E. J., Yaworski, E., et al. (2010) · Nature Biotechnology · doi:10.1038/nbt.1602 · Cited by 1,822 · cited 5× · see citing works
“study (12, 13, 27) in which liver-targeting siRNA-LNPs were formulated with 50% (14)C-labeled ionizable lipid, 10% DSPC, 38.”
BackgroundMethodInfluentialDelivery materials for siRNA therapeutics
Kanasty, R., Dorkin, J. R., Vegas, A., & Anderson, D. (2013) · Nature Materials · doi:10.1038/nmat3765 · Cited by 1,800 · see citing works
Self-citationModeling Tissue Morphogenesis and Cancer in 3D
Yamada, K. M., & Cukierman, E. (2007) · Cell · doi:10.1016/j.cell.2007.08.006 · Cited by 1,751 · see citing works
PEGylated nanoparticles for biological and pharmaceutical applications
Otsuka, H., Nagasaki, Y., & Kataoka, K. (2003) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(02)00226-0 · Cited by 1,587 · see citing works
Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms
Akinc, A., Querbes, W., De, S., Qin, J., Frank-Kamenetsky, M., Jayaprakash, K. N., Jayaraman, M., Rajeev, K. G., Cantley, W. L., Dorkin, J. R., et al. (2010) · Molecular Therapy · doi:10.1038/mt.2010.85 · Cited by 1,261 · see citing works
A combinatorial library of lipid-like materials for delivery of RNAi therapeutics
Akinc, A., Zumbuehl, A., Goldberg, M. S., Leshchiner, E. S., Busini, V., Hossain, N., Bacallado, S., Nguyen, D. N., Fuller, J. E., Alvarez, R., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1402 · Cited by 1,228 · see citing works
Self-citationLipid-like materials for low-dose, in vivo gene silencing
Love, K. T., Mahon, K. P., Levins, C. G., Whitehead, K. A., Querbes, W., Dorkin, J. R., Qin, J., Cantley, W., Qin, L. L., Racie, T., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0910603106 · Cited by 969 · see citing works
Self-citationSingle-cell RNA-seq: advances and future challenges
Saliba, A.-E., Westermann, A. J., Gorski, S. A., & Vogel, J. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku555 · Cited by 847 · see citing works
Endothelial Cell HeterogeneityPreprint
Aird, W. C. (2011) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a006429 · Cited by 729 · see citing works
Subcellular targeting strategies for drug design and delivery
Rajendran, L., Knölker, H., & Simons, K. (2009) · Nature Reviews Drug Discovery · doi:10.1038/nrd2897 · Cited by 709 · see citing works
In vivo endothelial siRNA delivery using polymeric nanoparticles with low molecular weight
Dahlman, J. E., Barnes, C., Khan, O. F., Thiriot, A., Jhunjhunwala, S., Shaw, T. E., Xing, Y., Sager, H. B., Sahay, G., Speciner, L., et al. (2014) · Nature Nanotechnology · doi:10.1038/nnano.2014.84 · Cited by 593 · see citing works
Self-citationDesigning Chemically Modified Oligonucleotides for Targeted Gene Silencing
Deleavey, G. F., & Damha, M. J. (2012) · Chemistry & Biology · doi:10.1016/j.chembiol.2012.07.011 · Cited by 590 · see citing works
Cationic lipid saturation influences intracellular delivery of encapsulated nucleic acids
Heyes, J., Palmer, L., Bremner, K., & MacLachlan, I. (2005) · Journal of Controlled Release · doi:10.1016/j.jconrel.2005.06.014 · Cited by 572 · see citing works
Mui, B. L., Tam, Y. K., Jayaraman, M., Ansell, S. M., Du, X., Tam, Y. Y. C., Lin, P. J. C., Chen, S., Narayanannair, J. K., Rajeev, K. G., et al. (2013) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2013.66 · Cited by 509 · see citing works
Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates
Dong, Y., Love, K. T., Dorkin, J. R., Sirirungruang, S., Zhang, Y., Chen, D., Bogorad, R. L., Yin, H., Chen, Y., Vegas, A. J., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1322937111 · Cited by 488 · see citing works
Self-citationA holistic approach to targeting disease with polymeric nanoparticles
Cheng, C. J., Tietjen, G. T., Saucier-Sawyer, J. K., & Saltzman, W. M. (2015) · Nature Reviews Drug Discovery · doi:10.1038/nrd4503 · Cited by 480 · see citing works
Chen, D. L., Love, K. T., Chen, Y., Eltoukhy, A. A., Kastrup, C. J., Sahay, G., Jeon, A., Dong, Y., Whitehead, K. A., & Anderson, D. G. (2012) · Journal of the American Chemical Society · doi:10.1021/ja301621z · Cited by 422 · see citing works
Self-citationMultiplexed Detection of Protein Cancer Markers with Biobarcoded Nanoparticle Probes
Stoeva, S. I., Lee, J., Smith, J., Rosen, S. T., & Mirkin, C. A. (2006) · Journal of the American Chemical Society · doi:10.1021/ja0613106 · Cited by 420 · see citing works
Polycation-siRNA nanoparticles can disassemble at the kidney glomerular basement membrane
Zuckerman, J. E., Choi, C. H. J., Han, H., & Davis, M. E. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1200718109 · Cited by 375 · see citing works
Multiplexed DNA Detection with Biobarcoded Nanoparticle Probes
Stoeva, S. I., Lee, J., Thaxton, C. S., & Mirkin, C. A. (2006) · Angewandte Chemie International Edition · doi:10.1002/anie.200600124 · Cited by 282 · see citing works
Mannocci, L., Zhang, Y., Scheuermann, J., Leimbacher, M., Bellis, G. D., Rizzi, E., Dumelin, C. E., Melkko, S., & Neri, D. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805130105 · Cited by 226 · see citing works
Sequence-Defined Polymers via Orthogonal Allyl Acrylamide Building Blocks
Porel, M., & Alabi, C. A. (2014) · Journal of the American Chemical Society · doi:10.1021/ja507262t · Cited by 221 · see citing works
The new paradigm of flow cell sequencing: Table 1.Preprint
Holt, R. A., & Jones, S. J. M. (2008) · Genome Research · doi:10.1101/gr.073262.107 · Cited by 217 · see citing works
Sager, H. B., Dutta, P., Dahlman, J. E., Hulsmans, M., Courties, G., Sun, Y., Heidt, T., Vinegoni, C., Borodovsky, A., Fitzgerald, K. J., et al. (2016) · Science Translational Medicine · doi:10.1126/scitranslmed.aaf1435 · Cited by 205 · see citing works
Self-citationCombinatorial synthesis of chemically diverse core-shell nanoparticles for intracellular delivery
Siegwart, D. J., Whitehead, K. A., Nuhn, L., Sahay, G., Cheng, H., Jiang, S., Ma, M., Lytton‐Jean, A. K. R., Vegas, A. J., Fenton, P., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1106379108 · Cited by 193 · see citing works
Self-citationSilencing or Stimulation? siRNA Delivery and the Immune System
Whitehead, K. A., Dahlman, J. E., Langer, R., & Anderson, D. G. (2011) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-061010-114133 · Cited by 192 · see citing works
Self-citationTheranostic barcoded nanoparticles for personalized cancer medicine
Yaari, Z. A., da Silva, D., Zinger, A., Goldman, E., Kajal, A., Tshuva, R., Barak, E., Dahan, N., Hershkovitz, D., Goldfeder, M., et al. (2016) · Nature Communications · doi:10.1038/ncomms13325 · Cited by 147 · see citing works
White, K., Lu, Y., Annis, S., Hale, A., Chau, B. N., Dahlman, J. E., Hemann, C. F., Opotowsky, A. R., Vargas, S. O., Rosas, I. O., et al. (2015) · EMBO Molecular Medicine · doi:10.15252/emmm.201404511 · Cited by 131 · see citing works
Self-citationIn Vitro–In Vivo Translation of Lipid Nanoparticles for Hepatocellular siRNA Delivery
Whitehead, K. A., Matthews, J., Chang, P., Niroui, F., Dorkin, J. R., Severgnini, M., & Anderson, D. G. (2012) · ACS Nano · doi:10.1021/nn301922x · Cited by 130 · see citing works
Self-citationHao, J., Kós, P., Zhou, K., Miller, J. B., Xue, L., Yan, Y., Xiong, H., Elkassih, S. A., & Siegwart, D. J. (2015) · Journal of the American Chemical Society · doi:10.1021/jacs.5b03429 · Cited by 104 · see citing works
Loss of α-catenin elicits a cholestatic response and impairs liver regeneration
Herr, K. J., Tsang, Y. N., Ong, J., Li, Q., Yap, L. L., Yu, W., Yin, H., Bogorad, R. L., Dahlman, J. E., Chan, Y. G., et al. (2014) · Scientific Reports · doi:10.1038/srep06835 · Cited by 40 · see citing works
Self-citationNanobarcoding: detecting nanoparticles in biological samples using in situ polymerase chain reaction
Leary, J. F., & Eustaquio (2012) · International Journal of Nanomedicine · doi:10.2147/ijn.s37433 · Cited by 10 · see citing works
Eustaquio, T., & Leary, J. F. (2015) · Cytometry Part A · doi:10.1002/cyto.a.22699 · Cited by 8 · see citing works
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Lipid nanoparticles for mRNA delivery
Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00358-0 · Cited by 4,076 · see citing works
Self-citationDrug delivery systems for RNA therapeutics
Paunovska, K., Loughrey, D., & Dahlman, J. E. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-021-00439-4 · Cited by 1,349 · see citing works
Self-citationvan der Meel, R., Sulheim, E., Shi, Y., Kiessling, F. M., Mulder, W. J. M., & Lammers, T. (2019) · Nature Nanotechnology · doi:10.1038/s41565-019-0567-y · Cited by 1,251 · see citing works
The role of lipid components in lipid nanoparticles for vaccines and gene therapy
Gregersen, C. H., Kulkarni, J. A., Witzigmann, D., Lind, M. L., Petersson, K., & Simonsen, J. B. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114416 · Cited by 1,215 · see citing works
Smart nanoparticles for cancer therapy
Sun, L., Liu, H., Ye, Y., Lei, Y., Islam, R., Tan, S., Tong, R., Miao, Y.-B., & Cai, L. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01642-x · Cited by 1,073 · see citing works
mRNA vaccine for cancer immunotherapy
Miao, L., Zhang, Y., & Huang, L. (2021) · Molecular Cancer · doi:10.1186/s12943-021-01335-5 · Cited by 947 · see citing works
Dilliard, S. A., & Siegwart, D. J. (2023) · Nature Reviews Materials · doi:10.1038/s41578-022-00529-7 · Cited by 707 · see citing works
Lipids and Lipid Derivatives for RNA Delivery
Zhang, Y., Sun, C., Wang, C., Jankovic, K. E., & Dong, Y. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00244 · Cited by 638 · see citing works
Lipid nanoparticles for nucleic acid delivery: Current perspectives
Samaridou, E., Heyes, J. A., & Lutwyche, P. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.06.002 · Cited by 593 · see citing works
Therapeutic in vivo delivery of gene editing agents
Raguram, A., Banskota, S., & Liu, D. R. (2022) · Cell · doi:10.1016/j.cell.2022.03.045 · Cited by 532 · see citing works
A framework for designing delivery systems
Poon, W., Kingston, B. R., Ouyang, B., Ngo, W., & Chan, W. C. W. (2020) · Nature Nanotechnology · doi:10.1038/s41565-020-0759-5 · Cited by 526 · see citing works
Integrating Artificial Intelligence and Nanotechnology for Precision Cancer Medicine
Adir, O., Poley, M., Chen, G., Froim, S., Krinsky, N., Shklover, J., Shainsky‐Roitman, J., Lammers, T., & Schroeder, A. (2020) · Advanced Materials · doi:10.1002/adma.201901989 · Cited by 496 · see citing works
Clinical translation of nanomedicines: Challenges, opportunities, and keys
Younis, M. A., Tawfeek, H. M., Abdellatif, A. A. H., Abdel-Aleem, J. A., & Harashima, H. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114083 · Cited by 494 · see citing works
Microfluidic formulation of nanoparticles for biomedical applications
Shepherd, S. J., Issadore, D., & Mitchell, M. J. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.120826 · Cited by 448 · see citing works
Brief update on endocytosis of nanomedicines
Patel, S., Kim, J., Herrera‐Barrera, M., Mukherjee, A., Kabanov, A. V., & Sahay, G. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.08.004 · Cited by 402 · see citing works
Targeting cancer with mRNA–lipid nanoparticles: key considerations and future prospects
Kon, E., Ad‐El, N., Hazan‐Halevy, I., Stotsky‐Oterin, L., & Peer, D. (2023) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-023-00811-9 · Cited by 385 · see citing works
Lipid nanoparticle technology for therapeutic gene regulation in the liver
Witzigmann, D., Kulkarni, J. A., Leung, J., Chen, S., Cullis, P. R., & van der Meel, R. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.06.026 · Cited by 376 · see citing works
Sago, C. D., Lokugamage, M. P., Paunovska, K., Vanover, D. A., Monaco, C. M., Shah, N. N., Gamboa Castro, M., Anderson, S. E., Rudoltz, T. G., Lando, G. N., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1811276115 · Cited by 313 · see citing works
Self-citationThe Biomolecular Corona of Lipid Nanoparticles for Gene Therapy
Francia, V., Schiffelers, R. M., Cullis, P. R., & Witzigmann, D. (2020) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.0c00366 · Cited by 306 · see citing works
Evolution and clinical translation of drug delivery nanomaterials
Hassan, S., Prakash, G., Bal‐Öztürk, A., Saghazadeh, S., Sohail, M. F., Seo, J., Dokmeci, M. R., Zhang, Y. S., & Khademhosseini, A. (2017) · Nano Today · doi:10.1016/j.nantod.2017.06.008 · Cited by 288 · see citing works
Paunovska, K., Sago, C. D., Monaco, C. M., Hudson, W. H., Castro, M. G., Rudoltz, T. G., Kalathoor, S., Vanover, D. A., Santangelo, P. J., Ahmed, R., et al. (2018) · Nano Letters · doi:10.1021/acs.nanolett.8b00432 · Cited by 277 · see citing works
Self-citationZhang, R., El-Mayta, R. D., Murdoch, T. J., Warzecha, C. C., Billingsley, M. M., Shepherd, S. J., Gong, N., Wang, L., Wilson, J. M., Lee, D., et al. (2020) · Biomaterials Science · doi:10.1039/d0bm01609h · Cited by 261 · see citing works
Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery
Vaughan, H. J., Green, J. J., & Tzeng, S. Y. (2019) · Advanced Materials · doi:10.1002/adma.201901081 · Cited by 257 · see citing works
Artificial intelligence aids in development of nanomedicines for cancer management
Tan, P., Chen, X., Zhang, H., Wei, Q., & Luo, K. (2023) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2023.01.005 · Cited by 254 · see citing works
Liu, D., Zhang, H., Fontana, F., Hirvonen, J. J., & Santos, H. A. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.08.003 · Cited by 238 · see citing works
Polymeric vehicles for nucleic acid delivery
Piotrowski-Daspit, A. S., Kauffman, A. C., Bracaglia, L. G., & Saltzman, W. M. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.06.014 · Cited by 224 · see citing works
Ionizable lipid nanoparticles encapsulating barcoded mRNA for accelerated in vivo delivery screening
Guimarães, P. P. G., Zhang, R., Spektor, R., Tan, M., Chung, A., Billingsley, M. M., El-Mayta, R. D., Riley, R., Wang, L., Wilson, J. M., et al. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.10.028 · Cited by 219 · see citing works
Massively parallel pooled screening reveals genomic determinants of nanoparticle delivery
Boehnke, N., Straehla, J. P., Safford, H. C., Kocak, M., Rees, M. G., Ronan, M. M., Rosenberg, D., Adelmann, C. H., Chivukula, R. R., Nabar, N., et al. (2022) · Science · doi:10.1126/science.abm5551 · Cited by 204 · see citing works
Biomaterials for vaccine-based cancer immunotherapy
Zhang, R., Billingsley, M. M., & Mitchell, M. J. (2018) · Journal of Controlled Release · doi:10.1016/j.jconrel.2018.10.008 · Cited by 201 · see citing works
Davis, J. R., Wang, X., Witte, I. P., Huang, T. P., Levy, J. M., Raguram, A., Banskota, S., Seidah, N. G., Musunuru, K., & Liu, D. R. (2022) · Nature Biomedical Engineering · doi:10.1038/s41551-022-00911-4 · Cited by 195 · see citing works
DNA Nanostructures: Current Challenges and Opportunities for Cellular Delivery
Lacroix, A., & Sleiman, H. F. (2021) · ACS Nano · doi:10.1021/acsnano.0c06136 · Cited by 194 · see citing works
Advances in Receptor‐Mediated, Tumor‐Targeted Drug Delivery
Large, D. E., Soucy, J. R., Hebert, J. M., & Auguste, D. T. (2018) · Advanced Therapeutics · doi:10.1002/adtp.201800091 · Cited by 189 · see citing works
Drug delivery systems for CRISPR-based genome editors
Madigan, V. J., Zhang, F., & Dahlman, J. E. (2023) · Nature Reviews Drug Discovery · doi:10.1038/s41573-023-00762-x · Cited by 180 · see citing works
Self-citationCRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics
Hao, L., Zhao, R. T., Welch, N. L., Tan, E. K. W., Zhong, Q., Harzallah, N. S., Ngambenjawong, C., Ko, H., Fleming, H. E., Sabeti, P. C., et al. (2023) · Nature Nanotechnology · doi:10.1038/s41565-023-01372-9 · Cited by 174 · see citing works
Loughrey, D., & Dahlman, J. E. (2022) · Accounts of Chemical Research · doi:10.1021/acs.accounts.1c00601 · Cited by 173 · see citing works
Self-citationFunctionalizing DNA origami to investigate and interact with biological systems
Knappe, G. A., Wamhoff, E., & Bathe, M. (2022) · Nature Reviews Materials · doi:10.1038/s41578-022-00517-x · Cited by 171 · see citing works
Machine Learning and Artificial Intelligence in Nanomedicine
Chou, W., Canchola, A., Zhang, F., & Lin, Z. (2025) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.70027 · Cited by 162 · see citing works
Paunovska, K., Gil, C. J., Lokugamage, M. P., Sago, C. D., Sato, M., Lando, G. N., Gamboa Castro, M., Bryksin, A. V., & Dahlman, J. E. (2018) · ACS Nano · doi:10.1021/acsnano.8b03640 · Cited by 160 · see citing works
Self-citationPeptide-Functionalized Lipid Nanoparticles for Targeted Systemic mRNA Delivery to the Brain
Han, E. L., Tang, S., Kim, D., Murray, A. M., Swingle, K. L., Hamilton, A. G., Mrksich, K., Padilla, M. S., Palanki, R., Li, J. J., et al. (2025) · Nano Letters · doi:10.1021/acs.nanolett.4c05186 · Cited by 158 · see citing works
Strategies for non-viral vectors targeting organs beyond the liver
Kim, J., Eygeris, Y., Ryals, R. C., Jozić, A., & Sahay, G. (2023) · Nature Nanotechnology · doi:10.1038/s41565-023-01563-4 · Cited by 154 · see citing works
Advancing engineering design strategies for targeted cancer nanomedicine
Gomerdinger, V. F., Nabar, N., & Hammond, P. T. (2025) · Nature reviews. Cancer · doi:10.1038/s41568-025-00847-2 · Cited by 154 · see citing works
Protein corona formed on lipid nanoparticles compromises delivery efficiency of mRNA cargo
Voke, E., Arral, M. L., Squire, H. J., Lin, T.-J., Zheng, L., Coreas, R., Lui, A., Iavarone, A. T., Pinals, R. L., Whitehead, K. A., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-63726-2 · Cited by 146 · see citing works
Xue, L., Hamilton, A. G., Zhao, G., Xiao, Z., El-Mayta, R. D., Han, X., Gong, N., Xiong, X., Xu, J., Figueroa‐Espada, C. G., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-45422-9 · Cited by 142 · see citing works
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Design of lipid-based nanoparticles for delivery of therapeutic nucleic acids
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Rhym, L. H., Manan, R. S., Koller, A. A., Stephanie, G., & Anderson, D. G. (2023) · Nature Biomedical Engineering · doi:10.1038/s41551-023-01030-4 · Cited by 134 · see citing works
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Enhancing RNA-lipid nanoparticle delivery: Organ- and cell-specificity and barcoding strategies
Wei, P.-S., Thota, N., John, G., Chang, E., Lee, S., Wang, Y., Ma, Z., Tsai, Y.-H., & Mei, K. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.08.030 · Cited by 130 · see citing works
Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia
Swingle, K. L., Hamilton, A. G., Safford, H. C., Geisler, H. C., Thatte, A. S., Palanki, R., Murray, A. M., Han, E. L., Mukalel, A. J., Han, X., et al. (2025) · Nature · doi:10.1038/s41586-024-08291-2 · Cited by 129 · see citing works
Nanoparticles That Deliver RNA to Bone Marrow Identified by in Vivo Directed Evolution
Sago, C. D., Lokugamage, M. P., Islam, F. Z., Krupczak, B. R., Sato, M., & Dahlman, J. E. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.8b08976 · Cited by 129 · see citing works
Self-citationIterative Design of Ionizable Lipids for Intramuscular mRNA Delivery
Tilstra, G., Couture‐Senécal, J., Lau, Y. M. A., Manning, A. M., Wong, D., Janaeska, W. W., Wuraola, T. A., Pang, J., & Khan, O. F. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.2c10670 · Cited by 129 · see citing works
Gautam, M., Jozic, A., Su, G. L.-N., Herrera-Barrera, M., Curtis, A., Arrizabalaga, S., Tschetter, W., Ryals, R. C., & Sahay, G. (2023) · Nature Communications · doi:10.1038/s41467-023-42189-3 · Cited by 123 · see citing works
Li, G., Chen, T., Dahlman, J. E., Eniola‐Adefeso, L., Ghiran, I. C., Kurre, P., Lam, W. A., Lang, J. K., Marbán, E., Martı́n, P., et al. (2023) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12305 · Cited by 123 · see citing works
Self-citationPrecision medicine: In vivo CAR therapy as a showcase for receptor-targeted vector platforms
Michels, A., Ho, N., & Buchholz, C. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.05.018 · Cited by 110 · see citing works
Clip Chemistry: Diverse (Bio)(macro)molecular and Material Function through Breaking Covalent Bonds
Shieh, P., Hill, M. R., Zhang, W., Kristufek, S. L., & Johnson, J. A. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01282 · Cited by 119 · see citing works
Pinals, R. L., Chio, L., Ledesma, F., & Landry, M. P. (2020) · The Analyst · doi:10.1039/d0an00633e · Cited by 115 · see citing works
Delivery of modified mRNA to damaged myocardium by systemic administration of lipid nanoparticles
Evers, M. J. W., Du, W., Yang, Q., Kooijmans, S. A. A., Vink, A., van Steenbergen, M., Vader, P., de Jager, S. C. A., Fuchs, S. A., Mastrobattista, E., et al. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.01.027 · Cited by 113 · see citing works
Oral mRNA delivery using capsule-mediated gastrointestinal tissue injections
Abramson, A., Kirtane, A. R., Shi, Y., Zhong, G., Collins, J. E., Tamang, S. M., Ishida, K., Hayward, A. M., Wainer, J., Rajesh, N. U., et al. (2022) · Matter · doi:10.1016/j.matt.2021.12.022 · Cited by 109 · see citing works
Self-citationMukai, H., Ogawa, K., Kato, N., & Kawakami, S. (2022) · Drug Metabolism and Pharmacokinetics · doi:10.1016/j.dmpk.2022.100450 · Cited by 108 · see citing works
Simonsen, J. B. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.04.022 · Cited by 107 · see citing works
A User’s Guide to Machine Learning for Polymeric Biomaterials
Meyer, T. A., Ramirez, C., Tamasi, M., & Gormley, A. J. (2022) · ACS Polymers Au · doi:10.1021/acspolymersau.2c00037 · Cited by 104 · see citing works
Dobrowolski, C. N., Paunovska, K., Echeverri, E. S., Loughrey, D., Sanchez, A. J. D. S., Ni, H., Hatit, M. Z. C., Lokugamage, M. P., Kuzminich, Y., Peck, H. E., et al. (2022) · Nature Nanotechnology · doi:10.1038/s41565-022-01146-9 · Cited by 103 · see citing works
Self-citationLipid Nanoparticles for Delivery of Therapeutic RNA Oligonucleotides
Moss, K. H., Popova, P. G., Hadrup, S. R., Astakhova, K., & Taskova, M. (2019) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.8b01290 · Cited by 99 · see citing works
Designing lipid nanoparticles using a transformer-based neural network
Chan, A., Kirtane, A. R., Qu, Q. R., Huang, X., Woo, J., Subramanian, D. A., Dey, R., Semalty, R., Bernstock, J. D., Ahmed, T., et al. (2025) · Nature Nanotechnology · doi:10.1038/s41565-025-01975-4 · Cited by 97 · see citing works
Engineering nanoparticle toolkits for mRNA delivery
Sun, B. F., Wu, W., Narasipura, E. A., Ma, Y., Yu, C., Fenton, O. S., & Song, H. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.115042 · Cited by 93 · see citing works
Frameworks for transformational breakthroughs in RNA-based medicines
Androsavich, J. R. (2024) · Nature Reviews Drug Discovery · doi:10.1038/s41573-024-00943-2 · Cited by 93 · see citing works
Soroudi, S., Jaafari, M. R., & Arabi, L. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.06.023 · Cited by 91 · see citing works
Applying artificial intelligence and computational modeling to nanomedicine
Hamilton, S., & Kingston, B. R. (2023) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2023.103043 · Cited by 90 · see citing works
Liang, G., Cao, W., Tang, D., Zhang, H., Yu, Y., Ding, J., Karges, J., & Xiao, H. (2024) · ACS Nano · doi:10.1021/acsnano.3c11154 · Cited by 88 · see citing works
Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases
da Silva, J. F., Tou, C. J., King, E. M., Eller, M. L., Rufino‐Ramos, D., Ma, L., Cromwell, C. R., Metović, J., Benning, F. M. C., Chao, L. H., et al. (2024) · Nature Biotechnology · doi:10.1038/s41587-024-02324-x · Cited by 83 · see citing works
Optimizing synthetic nucleic acid and protein nanocarriers: The chemical evolution approach
Freitag, F., & Wagner, E. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.03.005 · Cited by 82 · see citing works
Testing thousands of nanoparticles in vivo using DNA barcodes
Lokugamage, M. P., Sago, C. D., & Dahlman, J. E. (2018) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2018.08.001 · Cited by 81 · see citing works
Self-citationTargeted nonviral delivery of genome editors in vivo
Tsuchida, C. A., Wasko, K. M., Hamilton, J. R., & Doudna, J. A. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2307796121 · Cited by 79 · see citing works
Design of polymers for siRNA delivery: Recent progress and challenges
Wang, H., Zhang, S., Lv, J., & Cheng, Y. (2021) · Open Research (University of Surrey) · doi:10.1002/viw.20200026 · Cited by 78 · see citing works
Shi, Q., Yin, H., Song, R., Xu, J., Tan, J., Zhou, X. G., Cen, J., Deng, Z., Tong, H., Cui, C., et al. (2022) · Nature Chemistry · doi:10.1038/s41557-022-01076-y · Cited by 77 · see citing works
Gan, Z., Lokugamage, M. P., Hatit, M. Z. C., Loughrey, D., Paunovska, K., Sato, M., Cristian, A., & Dahlman, J. E. (2020) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10161 · Cited by 75 · see citing works
Self-citationUnlocking the Therapeutic Applicability of LNP-mRNA: Chemistry, Formulation, and Clinical Strategies
Huang, X., Ma, Y., Ma, G., & Xia, Y. (2024) · Research · doi:10.34133/research.0370 · Cited by 75 · see citing works
Raguram, A., An, M., Chen, P., & Liu, D. R. (2024) · Nature Biotechnology · doi:10.1038/s41587-024-02467-x · Cited by 75 · see citing works
Machine Learning‐Enhanced Nanoparticle Design for Precision Cancer Drug Delivery
Wang, Q., Liu, Y., Li, C., Xu, S., Xu, S., & Liu, B. (2025) · Advanced Science · doi:10.1002/advs.202503138 · Cited by 76 · see citing works
Multi-omics approaches to decipher the interactions of nanoparticles and biological systems
Wang, Y., Xiao, Z., Wang, Z., Lee, D., Ma, Y., Wilhelm, S., Wang, H., Kim, B. Y. S., & Jiang, W. (2024) · Nature Reviews Bioengineering · doi:10.1038/s44222-024-00264-4 · Cited by 74 · see citing works
Young, R. E., Nelson, K. M., Hofbauer, S. I., Vijayakumar, T., Alameh, M., Weissman, D., Papachristou, C., Gleghorn, J. P., & Riley, R. (2023) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.11.014 · Cited by 73 · see citing works
Cheng, L., Zhu, Y., Ma, J., Aggarwal, A., Toh, W. H., Shin, C., Sangpachatanaruk, W., Weng, G. W., Kumar, R., & Mao, H. (2024) · ACS Nano · doi:10.1021/acsnano.4c07615 · Cited by 73 · see citing works
Tissue-specific mRNA delivery and prime editing with peptide–ionizable lipid nanoparticles
Lin, Y., Li, M., Luo, Z., Meng, Y., Zong, Y., Ren, H., Yu, X., Tan, X., Liu, F., Wei, T., et al. (2025) · Nature Materials · doi:10.1038/s41563-025-02320-9 · Cited by 72 · see citing works
A holistic analysis of the intrinsic and delivery-mediated toxicity of siRNA therapeutics
Ranjbar, S., Zhong, X., Manautou, J. E., & Lü, X. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.115052 · Cited by 69 · see citing works
Using Large Datasets to Understand Nanotechnology
Paunovska, K., Loughrey, D., Sago, C. D., Langer, R., & Dahlman, J. E. (2019) · Advanced Materials · doi:10.1002/adma.201902798 · Cited by 67 · see citing works
Self-citationGong, D. H., Ben‐Akiva, E., Singh, A., Yamagata, H. M., Est-Witte, S. E., Shade, J. K., Trayanova, N. A., & Green, J. J. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.09.072 · Cited by 66 · see citing works
The Multifaceted Histidine-Based Carriers for Nucleic Acid Delivery: Advances and Challenges
He, J., Xu, S., & Mixson, A. J. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12080774 · Cited by 65 · see citing works
Huayamares, S. G., Lokugamage, M. P., Rab, R., Sanchez, A. J. D. S., Kim, H., Radmand, A., Loughrey, D., Lian, L., Hou, Y., Achyut, B. R., et al. (2023) · Journal of Controlled Release · doi:10.1016/j.jconrel.2023.04.005 · Cited by 65 · see citing works
Self-citationExploiting the placenta for nanoparticle-mediated drug delivery during pregnancy
Figueroa‐Espada, C. G., Hofbauer, S. I., Mitchell, M. J., & Riley, R. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.09.006 · Cited by 63 · see citing works
On‐Chip Spiral Waveguides for Ultrasensitive and Rapid Detection of Nanoscale Objects
Tang, S., Liu, S., Yu, X., Song, Q., Gong, Q., & Xiao, Y. (2018) · Advanced Materials · doi:10.1002/adma.201800262 · Cited by 63 · see citing works
Therapeutic RNA Delivery for COVID and Other Diseases
Dobrowolski, C. N., Paunovska, K., Hatit, M. Z. C., Lokugamage, M. P., & Dahlman, J. E. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202002022 · Cited by 62 · see citing works
Self-citationAdvanced Optical Imaging-Guided Nanotheranostics towards Personalized Cancer Drug Delivery
Murar, M., Albertazzi, L., & Pujals, S. (2022) · Nanomaterials · doi:10.3390/nano12030399 · Cited by 62 · see citing works
Werfel, T. A., Jackson, M. A., Kavanaugh, T. E., Kirkbride, K. C., Miteva, M., Giorgio, T. D., & Duvall, C. L. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.03.389 · Cited by 61 · see citing works
Mancardi, G., Mikołajczyk, A., Annapoorani, V. K., Bahl, A., Blekos, K., Burk, J., Çetin, Y. A., Chairetakis, K., Dutta, S., Escorihuela, L., et al. (2023) · Materials Today · doi:10.1016/j.mattod.2023.05.029 · Cited by 60 · see citing works
Application of lipid-based nanoparticles in cancer immunotherapy
Zhang, Z., Yao, S., Hu, Y., Zhao, X. B., & Lee, R. J. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.967505 · Cited by 57 · see citing works
Dynamic carriers for therapeutic RNA delivery
Berger, S., Lächelt, U., & Wagner, E. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2307799120 · Cited by 57 · see citing works
Nanoparticle-based DNA vaccine protects against SARS-CoV-2 variants in female preclinical models
Guimarães, L. C., Costa, P. A. C., Scalzo, S., Ferreira, H. A. S., Braga, A. C. S., de Oliveira, L. C., Figueiredo, M. M., Shepherd, S. J., Hamilton, A. G., Queiroz‐Junior, C. M., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-44830-1 · Cited by 56 · see citing works
Oxidized mRNA Lipid Nanoparticles for In Situ Chimeric Antigen Receptor Monocyte Engineering
Mukalel, A. J., Hamilton, A. G., Billingsley, M. M., Li, J. J., Thatte, A. S., Han, X., Safford, H. C., Padilla, M. S., Papp, T. E., Parhiz, H., et al. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202312038 · Cited by 56 · see citing works
Lipid Nanoparticles for Nucleic Acid Delivery Beyond the Liver
Saber, N., Sentí, M. E., & Schiffelers, R. M. (2024) · Human Gene Therapy · doi:10.1089/hum.2024.106 · Cited by 56 · see citing works
- Authors
- Dahlman, J. E., Kauffman, K. J., Xing, Y., Shaw, T. E., Mir, F. F., Dlott, C. C., Langer, R., Anderson, D. G., & Wang, E. T.
- Affiliations
- Georgia Institute of Technology (US)
- Koch Institute for Integrative Cancer Research At MIT (US)
- Harvard–MIT Division of Health Sciences and Technology (US)
- Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139;
- University of Florida (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-02-06
- Volume
- 114
- Issue
- 8
- Pages
- 2060-2065
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5338412
- OpenAlex
- W2586934391
- Cited by
- 335
- References
- 39
- Citations by year
- 2026: 50 · 2025: 62 · 2024: 53 · 2023: 43 · 2022: 40 · 2021: 22 · 2020: 24 · 2019: 13 · 2018: 18 · 2017: 10
- FWCI
- 7.26
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- Funders
- HHS | National Institutes of Health (DP5-OD017865)
- National Science Foundation (1122374)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
- Keywords
- nanoparticle biodistribution, siRNA delivery, structure-activity relationship
- MeSH terms
- Animals, Cell Separation, Pharmaceutical Preparations, Factor VII, Female, Flow Cytometry, Liver, Lung, Mice, Inbred C57BL, Nucleic Acids, Tissue Distribution, Drug Delivery Systems, RNA Interference, RNA, Small Interfering, Mice, Nanoparticles, Drug Discovery, DNA Barcoding, Taxonomic, Molecular Targeted Therapy
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