Wisteria floribunda agglutinin-labelled nets surround parvalbumin-containing neurons
Abstract
Net-like structures surrounding several types of neurones contain glycoconjugates which are detectable by lectins specific for N-acetylgalactosamine. Wisteria floribunda agglutinin (WFA) was introduced as a further marker for the visualization of such perineuronal nets, which were also revealed in regions of the rat brain where these structures could not be clearly demonstrated using other lectins. The WFA-labelled perineuronal nets resembled in detail those which could be visualized using Vicia villosa agglutinin, colloidal iron or hyaluronectin as markers. Furthermore, WFA-stained perineuronal net components appeared to be similar to proteoglycan-immunoreactive structures. Dual-peroxidase experiments and fluorescence double labelling demonstrated that WFA-binding structures frequently ensheath GABAergic neurons containing the calcium-binding protein parvalbumin in the areas investigated.
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PNNs are found primarily around fast-spiking, parvalbumin (PV)-containing GABAergic interneurons in many brain regions, but not all regions.
“PNNs are found primarily around fast--spiking, parvalbumin (PV)--containing GABAergic interneurons in many brain regions (Hartig et al., 1992;Schuppel et al., 2002;Dityatev et al., 2007), but not all regions (e.g., cerebellum; see below).”
From Casting a Wide Net: Role of Perineuronal Nets in Neural Plasticity (2016) · cites this paper as Hartig et al., 1992
PNNs surround inhibitory fast spiking parvalbumin-positive GABAergic interneurons and are also associated with excitatory pyramidal neurons in the cerebral cortex.
“PNNs surround inhibitory fast spiking parvalbumin-positive GABAergic interneurons12–14, but are also associated with further subpopulations, for example excitatory pyramidal neurons in the cerebral cortex15,16.”
From Elimination of the four extracellular matrix molecules tenascin-C, tenascin-R, brevican and neurocan alters the ratio of excitatory and inhibitory synapses (2019) · cites this paper as [13]
PNs frequently surround neurons containing parvalbumin, which are fast-firing GABAergic neurons co-expressing the voltage-gated potassium channel Kv3.1b.
“PNs frequently surround neurons containing parvalbumin (Kosaka and Heizmann, 1989; Härtig et al., 1992), known as a marker for fast-firing GABAergic neurons co-expressing the voltage-gated potassium channel Kv3.1b (Härtig et al., 1999).”
From Damaged Neocortical Perineuronal Nets Due to Experimental Focal Cerebral Ischemia in Mice, Rats and Sheep (2017) · cites this paper as Härtig et al., 1992
The majority of neurons surrounded by perineuronal nets are parvalbumin-expressing GABAergic interneurons.
“Strikingly, the majority of neurons surrounded by perineuronal nets are parvalbumin-expressing GABAergic interneurons [159]”
From Apolipoprotein E4, inhibitory network dysfunction, and Alzheimer’s disease (2019) · cites this paper as [159]
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- Authors
- Härtig, W., Brauer, K., & Brückner, G.
- Affiliations
- Leipzig University (DE)
- ORCID
- Härtig, Wolfgang
- Venue
- NeuroReport
- Published
- 1992-10
- Volume
- 3
- Issue
- 10
- Pages
- 869-872
- ISSN
- 0959-4965 (ISSN-L)
- OpenAlex
- W2065637628
- Cited by
- 440
- References
- 0
- Citations by year
- 2026: 14 · 2025: 23 · 2024: 34 · 2023: 17 · 2022: 21 · 2021: 30 · 2020: 23 · 2019: 24 · 2018: 29 · 2017: 25 · 2016: 23 · 2015: 12 · 2014: 18 · 2013: 10 · 2012: 7
- FWCI
- 1.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroreport (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Keywords
- perineuronal nets, Wisteria floribunda agglutinin, parvalbumin, GABAergic neurons, N-acetylgalactosamine, proteoglycans, rat brain
- MeSH terms
- Animals, Cerebral Cortex, Fluorescent Antibody Technique, Immunoenzyme Techniques, Microscopy, Fluorescence, Neurons, Parvalbumins, Rats, Wistar, Receptors, N-Acetylglucosamine, Lectins, Plant Lectins, Rats
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