Two Novel Proteins Activate Superoxide Generation by the NADPH Oxidase NOX1
Open accessAbstract
NOX1, an NADPH oxidase expressed predominantly in colon epithelium, shows a high degree of similarity to the phagocyte NADPH oxidase. However, superoxide generation by NOX1 has been difficult to demonstrate. Here we show that NOX1 generates superoxide when co-expressed with the p47(phox) and p67(phox) subunits of the phagocyte NADPH oxidase but not when expressed by itself. Since p47(phox) and p67(phox) are restricted mainly to myeloid cells, we searched for their homologues and identified two novel cDNAs. The mRNAs of both homologues were found predominantly in colon epithelium. Differences between the homologues and the phagocyte NADPH oxidase subunits included the lack of the autoinhibitory domain and the protein kinase C phosphorylation sites in the p47(phox) homologue as well as the absence of the first Src homology 3 domain and the presence of a hydrophobic stretch in the p67(phox) homologue. Co-expression of NOX1 with the two novel proteins led to stimulus-independent high level superoxide generation. Stimulus dependence of NOX1 was restored when p47(phox) was used to replace its homologue. In conclusion, NOX1 is a superoxide-generating enzyme that is activated by two novel proteins, which we propose to name NOXO1 (NOX organizer 1) and NOXA1 (NOX activator 1).
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The activator protein p47phox can replace NOXO1 in a noncanonical mode of NOX1 activation.
“The activator protein p47phox can replace NOXO1 in a noncanonical mode (61).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [61]
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- Authors
- Bánfi, B., Clark, R. A., Steger, K., & Krause, K.-H.
- Affiliations
- University Hospital of Geneva (CH)
- The University of Texas at San Antonio Health Science Center (US)
- Justus-Liebig-Universität Gießen (DE)
- Corresponding author
- Bánfi, Botond
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-02
- Volume
- 278
- Issue
- 6
- Pages
- 3510-3513
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2020319663
- Cited by
- 491
- References
- 27
- Citations by year
- 2026: 3 · 2025: 10 · 2024: 6 · 2023: 18 · 2022: 5 · 2021: 3 · 2020: 15 · 2019: 16 · 2018: 12 · 2017: 16 · 2016: 11 · 2015: 11 · 2014: 21 · 2013: 20 · 2012: 22
- FWCI
- 16.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion)
- Funders
- National Institute on Aging (R01AG019519, AG19519)
- National Institute of Allergy and Infectious Diseases (AI 20866, R01AI020866, R37AI020866)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- NOX1, NADPH oxidase, superoxide production, p47phox, p67phox, NOXO1, NOXA1, colonic epithelium, phagocyte NADPH oxidase, autoinhibitory domain, protein kinase C phosphorylation, Src homology 3 domain
- MeSH terms
- NADPH Oxidase 1, Amino Acid Sequence, Animals, Cell Line, Cloning, Molecular, Enzyme Activation, Humans, Molecular Sequence Data, NADH, NADPH Oxidoreductases, Proteins, RNA, Messenger, Superoxides, Sequence Homology, Amino Acid, Adaptor Proteins, Signal Transducing, Mice
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