Cited by 153Gut microbiota and healthAutism Spectrum Disorder ResearchChild Nutrition and Feeding Issues

A preliminary investigation on the relationship between gut microbiota and gene expressions in peripheral mononuclear cells of infants with autism spectrum disorders

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Inoue, Ryo, Sakaue, Yuko, Sawai, Chihiro, Sawai, Toshihiro, Ozeki, Motoyuki, Romero-Pérez, Gustavo A, Tsukahara, Takamitsu·2016·Bioscience, Biotechnology, and Biochemistry

Abstract

Fecal and blood samples of infants with autism spectrum disorders (ASD) and healthy infants were analyzed to investigate the association of altered gut microbiota and ASD development. 16S rRNA gene-based sequencing found that, unlike those of healthy infants, feces of ASD infants had significantly higher and lower abundance of genera Faecalibacterium and Blautia, respectively. Moreover, DNA microarray analysis of peripheral blood mononuclear cells (PBMC) detected more highly than low expressed genes in ASD infants than in healthy infants. Gene Ontology analysis revealed that differentially expressed genes between ASD and healthy infants were involved in interferon (IFN)-γ and type-I IFN signaling pathways. Finally, strong positive correlations between expression of IFN signaling-associated genes in PBMC and fecal abundance of Faecalibacterium were found. Our results strongly suggested that altered gut microbiota in infants resulted from ASD development and was associated with systemic immunity dysregulation, especially chronic inflammation.

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Crossref · 10.1080/09168451.2016.1222267

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Authors
Inoue, R., Sakaue, Y., Sawai, C., Sawai, T., Ozeki, M., Romero-Pérez, G. A., & Tsukahara, T.
Affiliations
  • Laboratory of Animal Science, Department of Agricultural and Life Sciences, Kyoto Prefectural University, Kyoto, Japan
  • Shiga University of Medical Science (JP)
  • Mukogawa Women's University (JP)
  • Kyoto Institute of Nutrition and Pathology, Kyoto, Japan
  • Kyoto Prefectural University (JP)
Venue
Bioscience, Biotechnology, and Biochemistry
Published
2016-12-01
Volume
80
Issue
12
Pages
2450-2458
ISSN
  • 0916-8451 (ISSN-L)
  • 1347-6947
OpenAlex
W2516846297
Cited by
148
References
69
Citations by year
2026: 9 · 2025: 12 · 2024: 23 · 2023: 25 · 2022: 11 · 2021: 26 · 2020: 14 · 2019: 13 · 2018: 7 · 2017: 7 · 2016: 1
FWCI
4.85
Type
journal article
Language
en
Open access
Locations
  • Bioscience Biotechnology and Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
The Morinaga Foundation for Health & Nutrition
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Autism Spectrum Disorder Research
  • Health Sciences › Medicine › Psychiatry and Mental health › Child Nutrition and Feeding Issues
Keywords
autism spectrum disorder, gut microbiota, infants, peripheral blood mononuclear cells, gene expression, Faecalibacterium, Blautia, interferon-gamma signaling, type I interferon signaling, chronic inflammation, fecal microbiota
MeSH terms
Autism Spectrum Disorder, Gastrointestinal Microbiome, Child, Preschool, Feces, Humans, Infant, Leukocytes, Mononuclear, Case-Control Studies, Transcriptome

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