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Cited by 904

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

P53 Binding Protein 1 (53bp1) Is an Early Participant in the Cellular Response to DNA Double-Strand Breaks

Open access
Schultz, Linda B., Chehab, Nabil H., Malikzay, Asra, Halazonetis, Thanos D.·2000·The Journal of Cell Biology

Abstract

p53 binding protein 1 (53BP1), a protein proposed to function as a transcriptional coactivator of the p53 tumor suppressor, has BRCT domains with high homology to the Saccharomyces cerevisiae Rad9p DNA damage checkpoint protein. To examine whether 53BP1 has a role in the cellular response to DNA damage, we probed its intracellular localization by immunofluorescence. In untreated primary cells and U2OS osteosarcoma cells, 53BP1 exhibited diffuse nuclear staining; whereas, within 5–15 min after exposure to ionizing radiation (IR), 53BP1 localized at discreet nuclear foci. We propose that these foci represent sites of processing of DNA double-strand breaks (DSBs), because they were induced by IR and chemicals that cause DSBs, but not by ultraviolet light; their peak number approximated the number of DSBs induced by IR and decreased over time with kinetics that parallel the rate of DNA repair; and they colocalized with IR-induced Mre11/NBS and γ-H2AX foci, which have been previously shown to localize at sites of DSBs. Formation of 53BP1 foci after irradiation was not dependent on ataxia-telangiectasia mutated (ATM), Nijmegen breakage syndrome (NBS1), or wild-type p53. Thus, the fast kinetics of 53BP1 focus formation after irradiation and the lack of dependency on ATM and NBS1 suggest that 53BP1 functions early in the cellular response to DNA DSBs.

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Metadata

Authors
Schultz, L. B., Chehab, N. H., Malikzay, A., & Halazonetis, T. D.
Venue
The Journal of Cell Biology
Published
2000-12-25
Volume
151
Issue
7
Pages
1381-1390
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
OpenAlex
W1978331091
Cited by
904
References
11
FWCI
6.76
Type
journal article
Language
en
Open access
bronze

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