PKA-activated ApAF–ApC/EBP heterodimer is a key downstream effector of ApCREB and is necessary and sufficient for the consolidation of long-term facilitation
Open accessAbstract
Long-term memory requires transcriptional regulation by a combination of positive and negative transcription factors. Aplysia activating factor (ApAF) is known to be a positive transcription factor that forms heterodimers with ApC/EBP and ApCREB2. How these heterodimers are regulated and how they participate in the consolidation of long-term facilitation (LTF) has not, however, been characterized. We found that the functional activation of ApAF required phosphorylation of ApAF by PKA on Ser-266. In addition, ApAF lowered the threshold of LTF by forming a heterodimer with ApCREB2. Moreover, once activated by PKA, the ApAF–ApC/EBP heterodimer transactivates enhancer response element–containing genes and can induce LTF in the absence of CRE- and CREB-mediated gene expression. Collectively, these results suggest that PKA-activated ApAF–ApC/EBP heterodimer is a core downstream effector of ApCREB in the consolidation of LTF.
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The induction and consolidation of LTF in vitro depends on the activity of several transcription factors.
“The induction and consolidation of LTF in vitro depends on the activity of several transcription factors24–27.”
From Computational design of enhanced learning protocols (2012) · cites this paper as [27]
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- Authors
- Lee, J.-A., Lee, S.-H., Lee, C., Chang, D.-J., Lee, Y., Kim, H., Cheang, Y.-H., Ko, H.-G., Lee, Y.-S., Jun, H., Bartsch, D., Kandel, E. R., & Kaang, B.-K.
- Affiliations
- Seoul National University (KR)
- 1Institute of Molecular Biology and Genetics, RIO, Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea
- 2Department of Molecular Biology, Central Institute of Mental Health, J5, 68 159 Mannheim, Germany
- Columbia University (US)
- ORCID
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2006-09-11
- Volume
- 174
- Issue
- 6
- Pages
- 827-838
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2064337
- OpenAlex
- W2113003079
- Cited by
- 25
- References
- 42
- Citations by year
- 2022: 2 · 2021: 1 · 2019: 3 · 2018: 1 · 2016: 2 · 2015: 1 · 2013: 1 · 2012: 1
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- Aplysia, protein kinase A, heterodimer, long-term facilitation, long-term memory consolidation, transcriptional regulation, gene transactivation
- MeSH terms
- Animals, Aplysia, Cells, Cultured, Gene Expression Regulation, Memory, Motor Neurons, Nerve Tissue Proteins, Nervous System, Synaptic Transmission, Neurons, Afferent, Phosphorylation, Repressor Proteins, Serine, Synapses, Transcription Factors, Signal Transduction, Transcriptional Activation, Cyclic AMP Response Element-Binding Protein, Long-Term Potentiation, Cyclic AMP-Dependent Protein Kinases, Dimerization, Response Elements
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