Aplysia CREB2 represses long-term facilitation: Relief of repression converts transient facilitation into long-term functional and structural change
Open accessAbstract
The switch from short- to long-term facilitation induced by behavioral sensitization in Aplysia involves CREB-like proteins, as well as the immediate-early gene ApC/EBP. Using the bZIP domain of ApC/EBP in a two-hybrid system, we have cloned ApCREB2, a transcription factor constitutively expressed in sensory neurons that resembles human CREB2 and mouse ATF4. ApCREB2 represses ApCREB1-mediated transcription in F9 cells. Injection of anti-ApCREB2 antibodies into Aplysia sensory neurons causes a single pulse of serotonin (5-HT), which induces only short-term facilitation lasting minutes, to evoke facilitation lasting more than 1 day. This facilitation has the properties of long-term facilitation: it requires transcription and translation, induces the growth of new synaptic connections, and occludes further facilitation by five pulses of 5-HT.
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LTF relies on deactivation of CREB2, a transcriptional repressor.
“LTF relies on deactivation of CREB2, a transcriptional repressor101.”
From The right time to learn: Mechanisms and optimization of spaced learning (2016) · cites this paper as [101]
Both PKA and ERK are needed to phosphorylate transcription factors critical for LTF, such as CREB1.
“These kinases are both needed to phosphorylate transcription factors critical for LTF (e.g., the transcriptional activator cAMP responsive element binding protein 1, CREB1)19,20.”
From Computational design of enhanced learning protocols (2012) · cites this paper as [19]
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- Authors
- Bartsch, D., Ghirardi, M., Skehel, P. A., Karl, K. A., Herder, S. P., Chen, M., Bailey, C. H., & Kandel, E. R.
- Affiliations
- Howard Hughes Medical Institute (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1995-12
- Volume
- 83
- Issue
- 6
- Pages
- 979-992
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2044126921
- Cited by
- 572
- References
- 62
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 13 · 2023: 10 · 2022: 20 · 2021: 9 · 2020: 6 · 2019: 12 · 2018: 12 · 2017: 17 · 2016: 18 · 2015: 13 · 2014: 20 · 2013: 25 · 2012: 24
- FWCI
- 11.20
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p01gm032099-14)
- National Institute of Mental Health (MH-37134, R01MH037134)
- National Institute of General Medical Sciences (GM 32099)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- ATF4, long-term facilitation, short-term facilitation, behavioral sensitization, Aplysia, transcriptional repression, sensory neurons, serotonin, synaptic plasticity, synaptic growth, bZIP transcription factors, CREB1
- MeSH terms
- Amino Acid Sequence, Animals, Aplysia, Base Sequence, Cells, Cultured, DNA, DNA-Binding Proteins, Humans, Memory, Molecular Sequence Data, Nerve Tissue Proteins, Neurons, Afferent, Nuclear Proteins, Organ Specificity, Phosphorylation, Repressor Proteins, RNA, Messenger, Serotonin, Synapses, Transcription Factors, Trans-Activators, Cyclic AMP Response Element-Binding Protein, Sequence Homology, Amino Acid, Long-Term Potentiation, CCAAT-Enhancer-Binding Proteins, Mice, Activating Transcription Factor 2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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