The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
An artificial chromosome for data storage
Open accessAbstract
Abstract DNA digital storage provides an alternative for information storage with high density and long-term stability. Here, we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip. The encoding paradigm utilizing the superposition of sparsified error correction codewords and pseudo-random sequences tolerates base insertions/deletions and is well suited to error-prone nanopore sequencing for data retrieval. The entire 254 kb sequence was 95.27% occupied by encoded data. The Transformation-Associated Recombination method was used in the construction of this chromosome from DNA fragments and necessary autonomous replication sequences. The stability was demonstrated by transmitting the data-carrying chromosome to the 100th generation. This study demonstrates a data storage method using encoded artificial chromosomes via in vivo assembly for write-once and stable replication for multiple retrievals, similar to a compact disc, with potential in economically massive data distribution.
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“Moreover, the dChr was replicated with high fidelity, no mutation appeared after the 100th generation of replication, while the encoding method used in this setup was tolerant toward a comparatively low accuracy of Nanopore sequencing, enabling the fast retrieval of reliable data [69].”
From DNA Data Storage. (2023) · cites this paper as [69]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
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“In 2021, Chen et al. created a circular 255 kbp yeast artificial chromosome (a data-carrying chromosome; dChr) encoding a total of 38 KB of digital data (two pictures and a video) [69].”
From DNA Data Storage. (2023) · cites this paper as [69]
Experimental — accuracy not yet measuredIn the citing paper
“Chen et al. (2021) encoded the information in a synthetic yeast artificial chromosome with four autonomously replicating sequences and maintained good stability during yeast replication.”
From Large-Scale de Novo Oligonucleotide Synthesis for Whole-Genome Synthesis and Data Storage: Challenges and Opportunities (2021) · cites this paper as Chen et al., 2021
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“It is also important to mention that the preservation of DNA digital archives can be implemented using not only in vitro substrates but also in vivo approaches.150−153”
From Emerging Approaches to DNA Data Storage: Challenges and Prospects (2022) · cites this paper as [150]
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Emerging Approaches to DNA Data Storage: Challenges and Prospects
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Metadata
- Authors
- Chen, W., Han, M., Zhou, J., Ge, Q., Wang, P., Zhang, X., Zhu, S., Song, L., & Yuan, Y.
- Affiliations
- Tianjin University (CN)
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China
- ORCID
- Venue
- National Science Review
- Publisher
- Oxford University Press (OUP)
- Published
- 2021-02-12
- Volume
- 8
- Issue
- 5
- Pages
- nwab028-nwab028
- ISSN
- 2053-714X (ISSN-L)
- 2095-5138
- PMC
- PMC8288405
- OpenAlex
- W3130820694
- Cited by
- 136
- References
- 43
- Citations by year
- 2026: 22 · 2025: 36 · 2024: 24 · 2023: 23 · 2022: 23 · 2021: 7
- FWCI
- 6.04
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- National Science Review (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (21621004)
- Topics
- Physical Sciences › Mathematics › Theoretical Computer Science › DNA and Biological Computing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- DNA data storage, artificial chromosomes, error correction coding, nanopore sequencing, transformation-associated recombination, in vivo assembly, autonomous replication sequence, data retrieval
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