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Weight loss regulates inflammation‐related genes in white adipose tissue of obese subjects
Abstract
Adipose tissue produces inflammation and immunity molecules suspected to be involved in obesity‐related complications. The pattern of expression and the nutritional regulation of these molecules in humans are poorly understood. We analyzed the gene expression profiles of subcutaneous white adipose tissue from 29 obese subjects during very low calorie diet (VLCD) using cDNA microarray and reverse transcription quantitative PCR. The patterns of expression were compared with that of 17 non‐obese subjects. We determined whether the regulated genes were expressed in adipocytes or stromavascular fraction cells. Gene expression profiling identified 100 inflammation‐related transcripts that are regulated in obese individuals when eating a 28 day VLCD but not a 2 day VLCD. Cluster analysis showed that the pattern of gene expression in obese subjects after 28 day VLCD was closer to the profile of lean subjects than to the pattern of obese subjects before VLCD. Weight loss improves the inflammatory profile of obese subjects through a decrease of proinflammatory factors and an increase of anti‐inflammatory molecules. The genes are expressed mostly in the stromavascular fraction of adipose tissue, which is shown to contain numerous macrophages. The beneficial effect of weight loss on obesity‐related complications may be associated with the modification of the inflammatory profile in adipose tissue.— Clément, K., Viguerie, N., Poitou, C., Carette, C., Pelloux, V., Curat, C. A., Sicard, A., Rome, S., Benis, A., Zucker, J.‐D., Vidal, H., Laville, M., Barsh, G. S., Basdevant, A., Stich, V., Cancello R., Langin, D. Weight loss regulates inflammation‐related genes in white adipose tissue of obese subjects. FASEB J. 18, 1657–1669 (2004)
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“Weight loss through reduced dietary intake and possibly bariatric surgery is associated with reductions in primary pro-inflammatory markers, in part owing to normalized expression of inflammation-related genes in white adipose tissue and to downregulation of the NLRP3 inflammasome58–61.”
From Inflammageing: chronic inflammation in ageing, cardiovascular disease, and frailty (2018) · cites this paper as [59]
In the citing paper
“Highly cross-linked AGE and melanoidins were predominantly excreted in the faeces while absorbed MRP were in general rapidly excreted by the kidney, with limited accumulation in tissues and organs (465,528 -531) .”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [531]
In the citing paper
“Adipose loss leads to the improvement of the inflammatory profile [11], with a concomitant reduction of infiltrating macrophages [13].”
From Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity (2008) · cites this paper as [11]
In the citing paper
“Although some molecules secreted by WAT, such as leptin and adiponectin, are synthesized by mature adipocytes [8], the non-adipose SVF, comprising infiltrated macrophages among other cellular types, is a source of inflammation-related molecules that may exert a local action on adipose tissue biology, particularly within the enlarged WAT [9-11].”
From Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity (2008) · cites this paper as [11]
In the citing paper
“We have shown previously that weight loss is associated with improvement in the inflammatory profile, together with regression of macrophage infiltration in WAT [11].”
From Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity (2008) · cites this paper as [11]
Citations
Cites
Gene Ontology: tool for the unification of biology
Ashburner, M., Ball, C. A., Blake, J. A., Botstein, D., Butler, H., Cherry, J. M., Davis, A. P., Dolinski, K., Dwight, S. S., Eppig, J. T., et al. (2000) · Nature Genetics · doi:10.1038/75556 · Cited by 43,206
Cluster analysis and display of genome-wide expression patterns
Eisen, M. B., Spellman, P. T., Brown, P. O., & Botstein, D. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.25.14863 · Cited by 16,184
In silico prediction of protein-protein interactions in human macrophages
Souiai, O., Guerfali, F. Z., Miled, S. B., Brun, C., & Benkahla, A. (2014) · BMC Research Notes · doi:10.1186/1756-0500-7-157 · Cited by 10,785
Significance analysis of microarrays applied to the ionizing radiation response
Tusher, V. G., Tibshirani, R., & Chu, G. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.091062498 · Cited by 10,568
Obesity is associated with macrophage accumulation in adipose tissue
Weisberg, S. P., McCann, D., Desai, M., Rosenbaum, M., Leibel, R. L., & Ferrante, A. W. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200319246 · Cited by 10,041
Adipose Expression of Tumor Necrosis Factor-α: Direct Role in Obesity-Linked Insulin Resistance
Hotamisligil, G. S., Shargill, N. S., & Spiegelman, B. M. (1993) · Science · doi:10.1126/science.7678183 · Cited by 7,518
Xu, H., Barnes, G. T., Yang, Q., Tan, G., Yang, D., Chou, C. J., Sole, J., Nichols, A., Ross, J. S., Tartaglia, L. A., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200319451 · Cited by 6,760
MANTOVANI, A., SOZZANI, S., LOCATI, M., ALLAVENA, P., & SICA, A. (2002) · Trends in Immunology · doi:10.1016/s1471-4906(02)02302-5 · Cited by 5,528
Chronic Subclinical Inflammation as Part of the Insulin Resistance Syndrome
Festa, A., D’Agostino, R., Howard, G., Mykkänen, L., Tracy, R. P., & Haffner, S. M. (2000) · Circulation · doi:10.1161/01.cir.102.1.42 · Cited by 2,366
Microarray data normalization and transformation
Quackenbush, J. (2002) · Nature Genetics · doi:10.1038/ng1032 · Cited by 1,975
Molecular Mechanisms of Amyloidosis
Merlini, G., & Bellotti, V. (2003) · New England Journal of Medicine · doi:10.1056/nejmra023144 · Cited by 1,955
Hsu, S.-M., Raine, L., & Fanger, H. (1981) · American Journal of Clinical Pathology · doi:10.1093/ajcp/75.5.734 · Cited by 1,638
Esposito, K., Pontillo, A., Di Palo, C., Giugliano, G., Masella, M., Marfella, R., & Giugliano, D. (2003) · JAMA · doi:10.1001/jama.289.14.1799 · Cited by 1,511
Monocyte chemoattractant protein 1 in obesity and insulin resistance
Sartipy, P., & Loskutoff, D. J. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1133870100 · Cited by 1,229
Bastard, J., Jardel, C., Bruckert, É., Blondy, P., Capeau, J., Laville, M., Vidal, H., & Hainque, B. (2000) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.85.9.6839 · Cited by 1,124
Self-citationZiccardi, P., Nappo, F., Giugliano, G., Esposito, K., Marfella, R., Cioffi, M., D’Andrea, F., Molinari, A. M., & Giugliano, D. (2002) · Circulation · doi:10.1161/hc0702.104279 · Cited by 994
Interleukin-10 Therapy—Review of a New Approach
Asadullah, K., Sterry, W., & Volk, H. (2003) · Pharmacological Reviews · doi:10.1124/pr.55.2.4 · Cited by 941
Smith, S. R., Lovejoy, J. C., Greenway, F., Ryan, D. H., de Jonge, L., de la Bretonne, J. A., Volafova, J., & Bray, G. A. (2001) · Metabolism · doi:10.1053/meta.2001.21693 · Cited by 612
Age-related inflammatory cytokines and disease
Brüünsgaard, H., & Pedersen, B. K. (2003) · Immunology and Allergy Clinics of North America · doi:10.1016/s0889-8561(02)00056-5 · Cited by 608
From Blood Monocytes to Adipose Tissue-Resident Macrophages
Curat, C. A., Miranville, A., Sengenès, C., Diehl, M., Tonus, C., Busse, R., & Bouloumié, A. (2004) · Diabetes · doi:10.2337/diabetes.53.5.1285 · Cited by 600
Self-citationPreadipocyte Conversion to Macrophage
Charrière, G. M., Cousin, B., Arnaud, E., André, M., Bacou, F., Pénicaud, L., & Casteilla, L. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m210811200 · Cited by 556
Leptin-specific patterns of gene expression in white adipose tissuePreprint
Soukas, A. A., Cohen, P., Socci, N. D., & Friedman, J. M. (2000) · Genes & Development · doi:10.1101/gad.14.8.963 · Cited by 513
Kopp, H. P., Kopp, C. W., Festa, A., Krzyzanowska, K., Kriwanek, S., Minar, E., Roka, R., & Schernthaner, G. (2003) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000073313.16135.21 · Cited by 427
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Grimble, R. F. (2002) · Current Opinion in Clinical Nutrition & Metabolic Care · doi:10.1097/00075197-200209000-00015 · Cited by 411
Chemokines control fat accumulation and leptin secretion by cultured human adipocytes
Gerhardt, C. C., Romero, I. A., Cancello, R., Camoin, L., & Strosberg, A. D. (2001) · Molecular and Cellular Endocrinology · doi:10.1016/s0303-7207(01)00394-x · Cited by 352
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Takahashi, K., Mizuarai, S., Araki, H., Mashiko, S., Ishihara, A., Kanatani, A., Itadani, H., & Kotani, H. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m309895200 · Cited by 327
Dusserre, E., Moulin, P., & Vidal, H. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/s0925-4439(99)00091-5 · Cited by 326
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Juge-Aubry, C. E., Somm, E., Giusti, V., Pernin, A., Chicheportiche, R., Verdumo, C., Rohner‐Jeanrenaud, F., Burger, D., Dayer, J., & Meier, C. A. (2003) · Diabetes · doi:10.2337/diabetes.52.5.1104 · Cited by 305
Bastard, J.-P., Jardel, C., Delattre, J., Hainque, B., Bruckert, E., & Oberlin, F. (1999) · Circulation · doi:10.1161/circ.99.16.2219/c · Cited by 244
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Gabrielsson, B. G., Johansson, J. M., Lönn, M., Jernås, M., Olbers, T., Peltonen, M., Larsson, I., Lönn, L., Sjöström, L., Carlsson, B., et al. (2003) · Obesity Research · doi:10.1038/oby.2003.100 · Cited by 218
Hotamışlıgil, G. S., Arner, P., Atkinson, R. L., & Spiegelman, B. M. (1997) · Diabetes · doi:10.2337/diab.46.3.451 · Cited by 209
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Mohamed‐Ali, V., Flower, L., Sethi, J. K., Hotamışlıgil, G. S., Gray, R. P., Humphries, S. E., York, D. A., & Pinkney, J. H. (2001) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.86.12.8104 · Cited by 204
Laimer, M. W., Ebenbichler, C. F., Kaser, S., Sandhofer, A., Weiß, H., Nehoda, H., Aigner, F., & Patsch, J. R. (2002) · International Journal of Obesity · doi:10.1038/sj.ijo.0801970 · Cited by 190
The role of chemokines in human cardiovascular pathology: enhanced biological insights
Shin, W. S., Szuba, A., & Rockson, S. G. (2002) · Atherosclerosis · doi:10.1016/s0021-9150(01)00571-8 · Cited by 190
Rome, S., Clément, K., Rabasa-Lhoret, R., Loizon, E., Poitou, C., Barsh, G. S., Riou, J.-P., Laville, M., & Vidal, H. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300293200 · Cited by 187
Self-citationColgan, S. P., Hershberg, R. M., Furuta, G. T., & Blumberg, R. S. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.24.13938 · Cited by 176
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Gómez‐Ambrosi, J., Catalán, V., Diez-Caballero, A., Martínez‐Cruz, L. A., Gil, M. J., García‐Foncillas, J., Cienfuegos, J. A., Salvador, J., Mato, J. M., & Frühbeck, G. (2003) · The FASEB Journal · doi:10.1096/fj.03-0591fje · Cited by 175
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Moraes, R., Blondet, A., Birkenkamp-Demtroeder, K., Tirard, J., Orntoft, T. F., Gertler, A., Durand, P., Naville, D., & Bégeot, M. (2003) · Endocrinology · doi:10.1210/en.2003-0456 · Cited by 152
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Harmelen, V. V., Hoffstedt, J., Lundquist, P., Vidal, H., Stemme, V., Hamsten, A., Arner, P., Reynisdottir, S., & Eriksson, P. (2000) · Thrombosis and Haemostasis · doi:10.1055/s-0037-1613860 · Cited by 88
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Argilés, J. M. A., & López-Soriano, F. J. (1998) · Current Opinion in Clinical Nutrition & Metabolic Care · doi:10.1097/00075197-199805000-00002 · Cited by 71
Marculescu, R., Endler, G., Schillinger, M., Iordanova, N., Exner, M., Hayden, E., Huber, K., Wagner, O. F., & Mannhalter, C. (2002) · Diabetes · doi:10.2337/diabetes.51.12.3582 · Cited by 70
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Hanusch‐Enserer, U., Cauza, E., Spak, M., Dunky, A., Rosén, H., Wolf, H., Prager, R., & Eibl, M. M. (2003) · International Journal of Obesity · doi:10.1038/sj.ijo.0802240 · Cited by 63
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Braat, H., Peppelenbosch, M. P., & Hommes, D. W. (2003) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.3.5.725 · Cited by 63
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Barreau, C., Cousin, B., André, M., Villena, J. A., Casteilla, L., & Pénicaud, L. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.08.034 · Cited by 58
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Metadata
- Authors
- ClÉment, K., Viguerie, N., Poitou, C., Carette, C., Pelloux, V., Curat, C. A., Sicard, A., Rome, S., Benis, A., Zucker, J., Vidal, H., Laville, M., Barsh, G. S., Basdevant, A., Stich, V., Cancello, R., & Langin, D.
- Affiliations
- Inserm (FR)
- Université Toulouse III - Paul Sabatier (FR)
- Goethe University Frankfurt (DE)
- Howard Hughes Medical Institute (US)
- Franco‐Czech Laboratory for Clinical Research on Obesity French Institute of Health and Medical Research (Inserm U586) and 3rd Faculty of Medicine, Charles University Prague Czech Republic
- ORCID
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2004-11
- Volume
- 18
- Issue
- 14
- Pages
- 1657-1669
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2160412430
- Cited by
- 640
- References
- 56
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 10 · 2023: 13 · 2022: 9 · 2021: 16 · 2020: 28 · 2019: 24 · 2018: 23 · 2017: 26 · 2016: 39 · 2015: 28 · 2014: 32 · 2013: 45 · 2012: 48
- FWCI
- 28.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Institut National de la Santé et de la Recherche Médicale (4NU10G)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Keywords
- weight loss, obesity, white adipose tissue, inflammation, gene expression profiling, very low calorie diet, subcutaneous adipose tissue, stromal vascular fraction, adipocytes, macrophages, pro-inflammatory genes, anti-inflammatory genes, adipose tissue inflammation
- MeSH terms
- Adipose Tissue, Adult, Female, Gene Expression Regulation, Humans, Inflammation, Obesity, Sialoglycoproteins, Weight Loss, Interleukin-10, Inflammation Mediators, Gene Expression Profiling, Caloric Restriction, Interleukin 1 Receptor Antagonist Protein
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