Distribution of Kisspeptin Neurones in the Adult Female Mouse Brain
Abstract
Kisspeptin‐GPR54 signalling is essential for normal reproductive functioning. However, the distribution of kisspeptin neuronal cell bodies and their projections is not well established. The present study aimed to provide a detailed account of kisspeptin neuroanatomy in the mouse brain. Using a polyclonal rabbit antibody AC566, directed towards the final ten C ‐terminal amino acids of murine kisspeptin, three populations of kisspeptin‐expressing cell bodies were identified in the adult female mouse brain. One exists as a dense periventricular continuum of cells within the rostral part of the third ventricle, another is found within the arcuate nucleus, and another is identified as a low‐density group of scattered cells within the dorsomedial nucleus and posterior hypothalamus. Kisspeptin‐immunoreactive fibres were abundant within the ventral aspect of the lateral septum and within the hypothalamus running in periventricular and ventral retrochiasmatic pathways. Notable exclusions from the kisspeptin fibre innervation were the suprachiasmatic and ventromedial nuclei. Outside of the hypothalamus, a small number of kisspeptin fibres were identified in the bed nucleus of the stria terminalis, subfornical organ, medial amygdala, paraventricular thalamus, periaqueductal grey and locus coerulus. All kisspeptin cell body and fibre immunoreactivity was absent in brain tissue from Kiss1 knockout mice. These observations provide a map of kisspeptin neurones in the mouse brain and indicate that a limited number of mostly medial hypothalamic and lateral septal brain regions are innervated by the three hypothalamic kisspeptin cell populations; the functions of these projections remain to be established.
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Kisspeptin neurons are located in the arcuate nucleus and the anteroventral periventricular nucleus.
“Kisspeptin neurons have been found in two main brain areas: the arcuate nucleus and the anteroventral periventricular nucleus (Clarkson, d’Anglemont de Tassigny, Colledge, Caraty, & Herbison, 2009; Clarkson & Herbison, 2006).”
From Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity (2017) · cites this paper as Clarkson, d’Anglemont de Tassigny, Colledge, Caraty, & Herbison, 2009
Cites
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Self-citationHypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54
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“In humans, loss of function mutations of GPR54 result in failed puberty and infertility (1, 2 ), whereas gain of function mutations result in precocious puberty (3).”
BackgroundRegulation of Kiss1 gene expression in the brain of the female mouse
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Kisspeptin Signaling in the Brain
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Epigenetic regulation of female puberty
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Self-citationComing of age in the Kisspeptin Era: Sex differences, development, and puberty
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Self-citationThe Origin of GnRH Pulse Generation: An Integrative Mathematical-Experimental Approach
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“Within the hypothalamus, two major kisspeptin producing neuronal populations are found in the arcuate nucleus (ARC) and in the preoptical area (Hrabovszky, 2014) or the anteroventral periventricular (AVPV)/rostral periventricular (PeN) continuum in rodents (Clarkson et al., 2009).”
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Derouiche, L., Keller, M., Martini, M., Duittoz, A. H., & Pillon, D. (2015) · Frontiers in Neuroscience · doi:10.3389/fnins.2015.00463 · Cited by 21 · see citing works
- Authors
- Clarkson, J., D’Anglemont de Tassigny, X., Colledge, W. H., Caraty, A., & Herbison, A. E.
- Affiliations
- University of Otago (NZ)
- University of Cambridge (GB)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Corresponding author
- Herbison, A. E.
- Venue
- Journal of Neuroendocrinology
- Publisher
- Wiley
- Published
- 2009-07-14
- Volume
- 21
- Issue
- 8
- Pages
- 673-682
- ISSN
- 0953-8194 (ISSN-L)
- 1365-2826
- OpenAlex
- W2157279176
- Cited by
- 346
- References
- 7
- Citations by year
- 2026: 8 · 2025: 17 · 2024: 18 · 2023: 8 · 2022: 22 · 2021: 23 · 2020: 13 · 2019: 24 · 2018: 25 · 2017: 25 · 2016: 21 · 2015: 17 · 2014: 26 · 2013: 34 · 2012: 23
- FWCI
- 24.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroendocrinology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council (BB/F01936X/1)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Keywords
- kisspeptin, GPR54, hypothalamus, arcuate nucleus, dorsomedial nucleus, lateral septum, neuroendocrine regulation of reproduction
- MeSH terms
- Receptors, Kisspeptin-1, Animals, Brain, Female, Humans, Immunohistochemistry, Mice, Inbred C57BL, Neurons, Proteins, Signal Transduction, Mice, Knockout, Receptors, G-Protein-Coupled, Mice, Kisspeptins
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