Effects of ecdysteroids on Chlorella vulgaris
Abstract
The effects of three ecdysteroids, 20‐hydroxyecdysone (20E), 2‐deoxy‐20‐hydroxyecdysone (2d20E) and 20‐hydroxyecdysone 22‐acetate (20E22Ac), on growth and the levels of cellular components in Chlorella vulgaris Beijerinck (Trebouxiophyceae) are reported and compared with data previously reported for ecdysone (E; Bajguz A and Koronka A, Plant Physiol Biochem 39: 707–715, 2001). All three 20‐hydroxyecdysteroids stimulate growth of C. vulgaris cells over a wide concentration range (10−16 to 10−7 M). Optimal stimulation is observed at 10−9 M with each ecdysteroid. High concentrations (>10−6 M) are cytotoxic. The potency ranking of the ecdysteroids is 20E > 20E22Ac > 2d20E > E. Levels per cell of DNA, RNA, protein, sugars, organic and inorganic phosphorus, chlorophylls a and b and phaeophytins a and b are all stimulated by ecdysteroid treatment when compared with the untreated control cells. Possible modes of action of ecdysteroids on C. vulgaris cells are discussed.
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Ecdysteroids seem to stimulate the size and growth of the alga Chlorella vulgaris and growth and heterocyst formation in the cyanobacterium Nostoc.
“Ecdysteroids seem to stimulate the size and growth of the alga Chlorella vulgaris [52,53] and growth and heterocyst formation in the cyanobacterium Nostoc [54].”
From Ecdysteroids (2021) · cites this paper as [53]
Exogenous ecdysone or 20-HE (10−10-10−8 M) facilitates growth and development of the alga Chlorella vulgaris.
“Exogenous ecdysone or 20-HE (10−10–10−8 M) facilitates the growth and development of the alga Chlorella vulgaris [174,175,176,177].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [175]
PEs (10−10-10−8 M) promote cell division in unicellular green algae and modulate physio-biochemical and metabolic responses.
“Similarly, PEs (10−10–10−8 M) promote cell division in unicellular green algae and modulate physio–biochemical and metabolic responses [175,177].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [175]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589 · see citing works
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684 · see citing works
THE COLORIMETRIC DETERMINATION OF PHOSPHORUS
Fiske, C. H., & Subbarow, Y. (1925) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)84756-1 · Cited by 19,155 · see citing works
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
Initiative, T. A. G. (2000) · Nature · doi:10.1038/35048692 · Cited by 9,301 · see citing works
Wellburn, A. R. (1994) · Journal of Plant Physiology · doi:10.1016/s0176-1617(11)81192-2 · Cited by 5,904 · see citing works
Somogyi, M. (1926) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)84465-9 · Cited by 5,601 · see citing works
Somogyi, M. (1952) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50870-5 · Cited by 5,310 · see citing works
Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues
Rogers, S. O., & Bendich, A. J. (1985) · Plant Molecular Biology · doi:10.1007/bf00020088 · Cited by 2,049 · see citing works
New insecticides with ecdysteroidal and juvenile hormone activity
Dhadialla, T. S., Carlson, G. R., & Le, D. P. (1998) · Annual Review of Entomology · doi:10.1146/annurev.ento.43.1.545 · Cited by 912 · see citing works
Ecdysone : from chemistry to mode of action
Koolman, J., Becker, J., & Karlson, P. (1989) · Medical Entomology and Zoology · Cited by 490 · see citing works
The structure of the nuclear hormone receptors
Kumar, R., & Thompson, E. B. (1999) · Steroids · doi:10.1016/s0039-128x(99)00014-8 · Cited by 433 · see citing works
The chemical characteristic and distribution of brassinosteroids in plants
Bajguz, A., & Tretyn, A. (2003) · Phytochemistry · doi:10.1016/s0031-9422(02)00656-8 · Cited by 432 · see citing works
Phytoecdysteroids: Biological aspects
Dinan, L. (2001) · Phytochemistry · doi:10.1016/s0031-9422(01)00078-4 · Cited by 392 · see citing works
Bajguz, A. (2000) · Plant Physiology and Biochemistry · doi:10.1016/s0981-9428(00)00733-6 · Cited by 246 · see citing works
Plant hormones and the control of physiological processes
Weyers, J. D. B., & Paterson, N. W. (2001) · New Phytologist · doi:10.1046/j.0028-646x.2001.00281.x · Cited by 208 · see citing works
Exploiting semiochemicals in insect control
Agelopoulos, N. G., Birkett, M. A., Hick, A. J., Hooper, A. M., Pickett, J. A., Pow, E. M., Smart, L. E., Smiley, D. W. M., Wadhams, L. J., & Woodcock, C. M. (1999) · Pesticide Science · doi:10.1002/(sici)1096-9063(199903)55:3 · Cited by 161 · see citing works
Steroid signaling in plants and insects—common themes, different pathways
Thummel, C. S., & Chory, J. (2002) · Genes & Development · doi:10.1101/gad.1042102 · Cited by 161 · see citing works
Prison, A., & Lorenzen, H. (1966) · Annual Review of Plant Physiology · doi:10.1146/annurev.pp.17.060166.002255 · Cited by 134 · see citing works
Blockade of heavy metals accumulation in Chlorella vulgaris cells by 24-epibrassinolide
Bajguz, A. (2000) · Plant Physiology and Biochemistry · doi:10.1016/s0981-9428(00)01185-2 · Cited by 102 · see citing works
Brassinosteroids and lead as stimulators of phytochelatins synthesis in Chlorella vulgaris
Bajguz, A. (2002) · Journal of Plant Physiology · doi:10.1078/0176-1617-00654 · Cited by 81 · see citing works
Activity of salicylic acid on the growth and biochemism of Chlorella vulgaris Beijerinck
Czerpak, R., Bajguz, A., Gromek, M., Kozłowska, G., & Nowak, I. (2002) · Acta Physiologiae Plantarum · doi:10.1007/s11738-002-0020-x · Cited by 54 · see citing works
Bajguz, A., & Czerpak, R. (1998) · Journal of Plant Growth Regulation · doi:10.1007/pl00007026 · Cited by 52 · see citing works
NISSEN, P. (1985) · Physiologia Plantarum · doi:10.1111/j.1399-3054.1985.tb08659.x · Cited by 46 · see citing works
Ecdysone-like metabolites, 14α-hydroxypinnasterols, from the red alga Laurencia pinnata
Fukuzawa, A., Miyamoto, M., Kumagai, Y., & Masamune, T. (1986) · Phytochemistry · doi:10.1016/s0031-9422(00)81278-9 · Cited by 40 · see citing works
Acetylpinnasterol and pinnasterol, ecdysone-like metabolites, from the marine red alga yamada
Fukuzawa, A., Kumagai, Y., Masamune, T., Furusaki, A., Katayama, C., & Matsumoto, T. (1981) · Tetrahedron Letters · doi:10.1016/s0040-4039(01)82071-2 · Cited by 39 · see citing works
Bajguz, A., & Czerpak, R. (1996) · Journal of Plant Growth Regulation · doi:10.1007/bf00198931 · Cited by 37 · see citing works
Voigt, B., Whiting, P., & Dinan, L. (2001) · Cellular and Molecular Life Sciences · doi:10.1007/pl00000927 · Cited by 34 · see citing works
Standardized abbreviations for common ecdysteroids
Lafont, R., Koolman, J., & Rees, H. H. (1993) · Insect Biochemistry and Molecular Biology · doi:10.1016/0965-1748(93)90103-y · Cited by 21 · see citing works
Two-dimensional thin-layer chromatography of plant ecdysteroids
Báthori, M., Blunden, G., & Kalász, H. (2000) · Chromatographia · doi:10.1007/bf02491010 · Cited by 19 · see citing works
Effect of ecdysone application on the growth and biochemical changes in Chlorella vulgaris cells
Bajguz, A., & Koronka, A. (2001) · Plant Physiology and Biochemistry · doi:10.1016/s0981-9428(01)01287-6 · Cited by 18 · see citing works
Light-Induced Cell Division in Chlorella Vulgaris, Beijerinck (Emerson Strain)
GRIFFITHS, D. J. (1961) · Annals of Botany · doi:10.1093/oxfordjournals.aob.a083746 · Cited by 17 · see citing works
The Effect of Glucose on Cell Division in Chlorella vulgaris, Beijerinck (Emerson Strain)
GRIFFITHS, D. J. (1963) · Annals of Botany · doi:10.1093/oxfordjournals.aob.a083865 · Cited by 12 · see citing works
Biochemical genetics of plant growth
Gray, W. M., & Estelle, M. A. (1998) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(98)80115-8 · Cited by 11 · see citing works
Gigantism-inducing Factors in Chlorella pyrenoidosa
Rodríguez-López, M. (1963) · Nature · doi:10.1038/199506a0 · Cited by 11 · see citing works
Bajguz, A., Czerpak, R., Piotrowska, A., & Polecka, M. (2013) · Acta Societatis Botanicorum Poloniae · doi:10.5586/asbp.2001.032 · Cited by 9 · see citing works
The effect of ecdysterone on the cyanobacterium Nostoc 6720
Maršálek, B., Šimek, M., & Smith, R. J. (1992) · Zeitschrift für Naturforschung C · doi:10.1515/znc-1992-9-1015 · Cited by 7 · see citing works
A Giant Form of Chlorella pyrenoidosa
R-LÓPEZ, M., & R-CANDELA, R. (1962) · Nature · doi:10.1038/196691a0 · Cited by 5 · see citing works
Flagged first, then by citations
4 of the 42 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Phytoecdysteroids: Diversity, biosynthesis and distribution
Dinan, L., Harmatha, J., Volodin, V., & Lafont, R. (2009) · Ecdysone: Structures and Functions · doi:10.1007/978-1-4020-9112-4_1 · Cited by 77 · see citing works
Effects of nonylphenol on the growth and microcystin production of Microcystis strains
Wang, J., Xie, P., & Guo, N. (2006) · Environmental Research · doi:10.1016/j.envres.2006.05.013 · Cited by 76 · see citing works
Ecdysteroids in Spinach (Spinacia oleracea L.): Biosynthesis, Transport and Regulation of Levels
Bakrim, A., Maria, A., Sayah, F., Lafont, R., & Takvorian, N. (2008) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2008.06.002 · Cited by 63 · see citing works
Tarkowská, D., & Strnad, M. (2016) · Planta · doi:10.1007/s00425-016-2561-z · Cited by 56 · see citing works
Lafont, R., Balducci, C., & Dinan, L. (2021) · Encyclopedia · doi:10.3390/encyclopedia1040096 · Cited by 49 · see citing works
Self-citationArif, Y., Singh, P., Bajguz, A., & Hayat, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23158664 · Cited by 50 · see citing works
Ecdysteroids in Plants and their Pharmacological Effects in Vertebrates and Humans
(2015) · Studies in Natural Products Chemistry · doi:10.1016/b978-0-444-63473-3.00005-8 · Cited by 48 · see citing works
20-Hydroxyecdysone protects wheat seedlings (Triticum aestivum L.) against lead stress
Lamhamdi, M., Lafont, R., Rharrabe, K., Sayah, F., Aarab, A., & Bakrim, A. (2016) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2015.11.006 · Cited by 18 · see citing works
Krespach, M. K. C., Stroe, M. C., Flak, M., Komor, A. J., Nietzsche, S., Sasso, S., Hertweck, C., & Brakhage, A. A. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2100892118 · Cited by 17 · see citing works
Ecdysteroids as defensive chemicals
(2022) · Advances in Insect Physiology · doi:10.1016/bs.aiip.2022.09.001 · Cited by 11 · see citing works
Gorelick-Feldman, J. (2009) · Rutgers University Community Repository (Rutgers University) · doi:10.7282/t3wq041h · Cited by 10 · see citing works
Janeczko, A., Oklestkova, J., Tarkowská, D., & Drygaś, B. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22062855 · Cited by 4 · see citing works
Antibacterial Compound Isolation and Characterization from the Plant Cyanotis axillaris Schult
Singh, K., Yadava, R. N., & Yadav, R. (2023) · Chemistry & Biodiversity · doi:10.1002/cbdv.202301094 · Cited by 4 · see citing works
Ufimtsev, K. G., Ширшова, Т. И., Volodin, V. V., Volodina, S. O., Alekseev, A. A., & Раушенбах, И. Ю. (2006) · Doklady Biological Sciences · doi:10.1134/s0012496606060263 · Cited by 3 · see citing works
Li-rong, Z., Linglong, H., Hu, Y., Jingguo, S., & Chen, W. (2010) · PubMed · Cited by 2 · see citing works
Hola, D., Frimlová, K., Kočová, M., Marková, H., Rothová, O., & Tůmová, L. (2020) · Photosynthetica · doi:10.32615/ps.2020.050 · Cited by 2 · see citing works
Potential use of Ecdysone in protecting wheat (Triticum aestivum) germination under cadmium stress
Lamhamdi, M., Gharbi, S., Arras, M. E., Galiou, O. E., Moudden, H. E., Lee, L., Bouyahya, A., & Bakrim, A. (2024) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2024.103479 · Cited by 2 · see citing works
- Authors
- Bajguz, A., & Dinan, L.
- Affiliations
- University of Białystok (PL)
- University of Exeter (GB)
- Corresponding author
- Bajguz, Andrzej
- Venue
- Physiologia Plantarum
- Publisher
- Wiley
- Published
- 2004-06-23
- Volume
- 121
- Issue
- 3
- Pages
- 349-357
- ISSN
- 0031-9317 (ISSN-L)
- 1399-3054
- OpenAlex
- W2009573807
- Cited by
- 17
- References
- 42
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 1 · 2016: 1 · 2015: 2
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Physiologia Plantarum (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Growth Enhancement Techniques
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- ecdysteroids, 20-hydroxyecdysone, Chlorella vulgaris, cytotoxicity, DNA content, RNA content, protein content, phosphorus, chlorophyll, phaeophytin
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