Cited by 251Gut microbiota and healthClostridium difficile and Clostridium perfringens researchOral microbiology and periodontitis research

Culture‐independent analysis of the gut microbiota in colorectal cancer and polyposis

Scanlan, Pauline D., Shanahan, Fergus, Clune, Yvonne, Collins, John K., O'Sullivan, Gerald C., O'Riordan, Micheal, Holmes, Elaine, Wang, Yulan +1 more·2008·Environmental Microbiology

Abstract

Summary: A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As the majority of bacteria in the gut are refractory to culture we investigated this ecosystem in subjects with colorectal cancer and with adenomatous polyposis who are at high risk of developing colorectal cancer, using culture‐independent methods. Twenty colorectal cancer and 20 polypectomized volunteers were chosen for this analysis. An exploration of the diversity and temporal stability of the dominant bacteria and several bacterial subgroups was undertaken using 16S rRNA gene denaturing gradient gel electrophoresis and ribosomal intergenic spacer analysis (RISA). Metabonomic analysis of the distal gut microbiota's environment was also undertaken. A significantly reduced temporal stability and increased diversity for the microbiota of subjects with colorectal cancer and polyposis was evident. A significantly increased diversity of the Clostridium leptum and C. coccoides subgroups was also noted for both disease groups. A clear division in the metabonome was observed for the colorectal cancer and polypectomized subjects compared with control volunteers. The intestinal microbiota and their metabolites are significantly altered in both colorectal cancer and polypectomized subjects compared with controls.

Integrity signals

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Crossref · 10.1111/j.1462-2920.2007.01503.x

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  • Several changes in microbiota composition are described in CRC: increased diversity in Clostridium leptum and Clostridium coccoides, while temporary instability and decreased complexity in composition are linked to colitis-associated CRC.

    “An increased diversity in Clostridium leptum (C.leptum) and Clostridium coccoides is present, while temporary instability and decreased complexity in composition are linked to colitis-associated CRC [48,49] .”
  • Reduced temporal stability and increased diversity has been shown for the faecal microbiota of subjects with established colorectal cancer and polyposis.

    “Reduced temporal stability and increased diversity has been shown for the faecal microbiota of subjects with established CRC and polyposis,86”

    From The gut microbiota and host health: a new clinical frontier (2016) · cites this paper as [86]

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Authors
Scanlan, P. D., Shanahan, F., Clune, Y., Collins, J. K., O'Sullivan, G. C., O'Riordan, M., Holmes, E., Wang, Y., & Marchesi, J. R.
Affiliations
  • University College Cork (IE)
  • Imperial College London (GB)
Corresponding author
Marchesi, Julian R.
Venue
Environmental Microbiology
Publisher
Wiley
Published
2008-01-25
Volume
10
Issue
3
Pages
789-798
ISSN
  • 1462-2912 (ISSN-L)
  • 1462-2920
OpenAlex
W2146480823
Cited by
245
References
47
Citations by year
2026: 3 · 2025: 4 · 2024: 4 · 2023: 6 · 2022: 8 · 2021: 12 · 2020: 10 · 2019: 14 · 2018: 20 · 2017: 13 · 2016: 17 · 2015: 23 · 2014: 22 · 2013: 26 · 2012: 12
FWCI
6.96
Type
journal article
Language
en
Update notices
erratum (2008-04-04)
Open access
closed
Locations
  • Environmental Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Health Research Board
  • Higher Education Authority
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Keywords
gut microbiota, colorectal cancer, adenomatous polyposis, polypectomy, microbial diversity, temporal stability, Clostridium leptum, Clostridium coccoides, 16S rRNA gene, RISA, metabolomics, cancer etiology
MeSH terms
Clostridium, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Female, Humans, Intestines, Male, Adenomatous Polyposis Coli, RNA, Ribosomal, 16S, Colorectal Neoplasms, DNA, Ribosomal Spacer, Gastrointestinal Tract, Biodiversity

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