Gene expression profiling of aging in multiple mouse strains: identification of aging biomarkers and impact of dietary antioxidants
Open accessAbstract
Summary: We used DNA microarrays to identify panels of transcriptional markers of aging that are differentially expressed in young (5 month) and old (25 month) mice of multiple inbred strains (129sv, BALB/c, CBA, DBA, B6, C3H and B6C3F 1 ). In the heart, age‐related changes of five genes were studied throughout the mouse lifespan: complement component 4, chemokine ligand 14, component of Sp100‐rs, phenylalanine hydroxylase and src family associated phosphoprotein 2. A similar analysis in the brain (cerebellum) involved complement component 1q (alpha polypeptide), complement component 4, P lysozyme structural, glial fibrillary acidic protein and cathepsin S. Caloric restriction (CR) inhibited age‐related expression of these genes in both tissues. Parametric analysis of gene set enrichment identified several biological processes that are induced with aging in multiple mouse strains. We also tested the ability of dietary antioxidants to oppose these transcriptional markers of aging. Lycopene, resveratrol, acetyl‐ l ‐carnitine and tempol were as effective as CR in the heart, and α‐lipoic acid and coenzyme Q 10 were as effective as CR in the cerebellum. These findings suggest that transcriptional biomarkers of aging in mice can be used to estimate the efficacy of aging interventions on a tissue‐specific basis.
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Caloric restriction prevents many age-dependent gene expression changes in the mouse brain, reduces age-related brain atrophy in rhesus macaques, and has significant beneficial effects on the age-dependent impairment of learning and memory in rodents and humans.
“It prevents many age-dependent gene expression changes in the mouse brain (8,43), reduces age-related brain atrophy in rhesus macaques (75) and has significant beneficial effects on the age-dependent impairment of learning and memory in rodents (76,77) and humans (78).”
From Neural mechanisms of ageing and cognitive decline (2010) · cites this paper as [43]
Caloric restriction has beneficial effects on the function of the brain and its vulnerability to age-dependent pathology.
“Caloric restriction has also been widely documented to have beneficial effects on the function of the brain and its vulnerability to age-dependent pathology.”
From Neural mechanisms of ageing and cognitive decline (2010) · cites this paper as [43]
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- Authors
- Park, S., Kim, K., Page, G. P., Allison, D. B., Weindruch, R., & Prolla, T. A.
- Affiliations
- University of Wisconsin–Madison (US)
- University of Alabama (US)
- Corresponding author
- Prolla, Tomas A.
- Venue
- Aging Cell
- Publisher
- Wiley
- Published
- 2009-07-21
- Volume
- 8
- Issue
- 4
- Pages
- 484-495
- ISSN
- 1474-9718 (ISSN-L)
- 1474-9726
- PMC
- PMC2733852
- OpenAlex
- W2145724295
- Cited by
- 134
- References
- 73
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 7 · 2023: 7 · 2022: 2 · 2021: 7 · 2020: 6 · 2019: 3 · 2018: 2 · 2017: 10 · 2016: 10 · 2015: 11 · 2014: 6 · 2013: 10 · 2012: 15
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Aging Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (R01AG020681)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
- Keywords
- aging, gene expression profiling, aging biomarkers, caloric restriction, dietary antioxidants, mouse strains, heart, cerebellum, complement C4, phenylalanine hydroxylase, glial fibrillary acidic protein, cathepsin S, lycopene, resveratrol, alpha-lipoic acid, coenzyme Q10, acetyl-L-carnitine, tempol, age-related gene expression
- MeSH terms
- Aging, Animals, Antioxidants, Brain, Diet, Kinetics, Male, Mice, Inbred Strains, Myocardium, Organ Specificity, Transcription, Genetic, Biomarkers, Gene Expression, Gene Expression Profiling, Caloric Restriction, Mice
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