Cited by 413Neuroinflammation and Neurodegeneration MechanismsGenetics, Aging, and Longevity in Model OrganismsNeuroscience and Neuropharmacology Research

GFAP mRNA increases with age in rat and human brain

Nichols, Nancy R., Day, Jonathan R., Laping, Nicholas J., Johnson, Steven A., Finch, Caleb E.·1993·Neurobiology of Aging

Abstract

Glial fibrillary acidic protein (GFAP) mRNA was examined by RNA blot hybridization in three age groups of two cohorts of male F-344 rats and in 47 human postmortem brain samples. GFAP mRNA increased in the hippocampus and striatum of 24 versus 6- to 7-month-old rats. Another astrocytic molecular marker, glutamine synthetase mRNA, did not change with age in rat brain. Rat GFAP mRNA prevalence was inversely correlated with serum testosterone but not correlated with serum corticosterone. In human hippocampus, frontal and temporal cortex, GFAP mRNA also increased in older (60-79 years) compared with middle-aged (25-59 years) individuals. In contrast, mitochondrial cytochrome oxidase subunit 1 mRNA did not change between age groups in any region. By combining the three regions for further analysis, GFAP mRNA increased with age irregardless of gender, alcoholism in the middle-aged group, or whether brains were classified as normal or neuropathologic (excluding Alzheimer's disease pathology). These data indicate that increased GFAP protein or GFAP-immunoreactive astrocytes in rats and humans may result from transcriptional or post-transcriptional regulation and extend the number to three species (including mouse) showing an increase in GFAP mRNA with age. Factors that are known to regulate GFAP mRNA expression in young brains are considered as possible causes of age-related increases.

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Crossref · 10.1016/0197-4580(93)90100-p

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Authors
Nichols, N. R., Day, J. R., Laping, N. J., Johnson, S. A., & Finch, C. E.
Affiliations
University of Southern California (US)
Corresponding author
Nichols, Nancy R.
Venue
Neurobiology of Aging
Publisher
Elsevier BV
Published
1993-09
Volume
14
Issue
5
Pages
421-429
ISSN
0197-4580 (ISSN-L)
OpenAlex
W2027953381
Cited by
413
References
62
Citations by year
2026: 4 · 2025: 19 · 2024: 21 · 2023: 27 · 2022: 22 · 2021: 19 · 2020: 21 · 2019: 16 · 2018: 19 · 2017: 16 · 2016: 7 · 2015: 10 · 2014: 10 · 2013: 9 · 2012: 11
FWCI
5.02
Type
journal article
Language
en
Open access
closed
Locations
  • Neurobiology of Aging (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r35ag007909-03, 5p30hd019445-05, 5r01ag010673-03)
  • National Institute on Aging (AG07909, AG 10673)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD 5 P30 HD19445)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
glial fibrillary acidic protein, GFAP mRNA, brain aging, astrocytes, hippocampus, striatum, glutamine synthetase, cytochrome c oxidase subunit I, testosterone, corticosterone, postmortem brain, F-344 rats, alcoholism, transcriptional regulation, post-transcriptional regulation
MeSH terms
Adult, Aged, Aging, Animals, Brain Chemistry, Female, Glial Fibrillary Acidic Protein, Humans, Male, Middle Aged, RNA, Messenger, Rats

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