Cited by 87Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchRedox biology and oxidative stress

Proteomic identification of nitrated brain proteins in amnestic mild cognitive impairment: a regional study

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Sultana, Rukhsana, Reed, Tanea, Perluigi, Marzia, Coccia, Rafaella, Pierce, William M., Butterfield, D. Allan·2007·Journal of Cellular and Molecular Medicine

Abstract

Oxidative stress is an imbalance between the level of antioxidants and oxidants in a cell. Oxidative stress has been shown in brain of subjects with mild cognitive impairment (MCI) as well Alzheimer's disease (AD). MCI is considered as a transition phase between control and AD. The focus of the current study was to identify nitrated proteins in the hippocampus and inferior parietal lobule (IPL) brain regions of subjects with amnestic MCI using proteomics. The identified nitrated proteins in MCI brain were compared to those previously reported to be nitrated and oxidatively modified in AD brain, a comparison that might provide an invaluable insight into the progression of the disease.

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Crossref · 10.1111/j.1582-4934.2007.00065.x

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  • Oxidative modifications to aconitase, creatine kinase, and ATP synthase in brain mitochondria help explain reduced glucose metabolism and ATP production in amnestic mild cognitive impairment and Alzheimer disease.

    “In addition, oxidative modifications to aconitase, a key iron-sulfur containing enzyme in the TCA cycle, creatine kinase (an enzyme which helps neurons to maintain ATP levels) and ATP synthase in brain mitochondria, help to explain decreased glucose metabolism and consequent decreased ATP production in the brains of people with aMCI and AD12,25,34.”

    From Oxidative stress, dysfunctional glucose metabolism and Alzheimer disease (2019) · cites this paper as [25]

    The Discussion’s “Energy or mitochondrial dysfunction” section links nitration of energy-metabolism proteins in amnestic MCI to possible metabolic disruption and notes reduced cerebral glucose utilization in AD (page unavailable). But it identifies enolase, aldolase, a glucose-regulated protein, and MDH—not oxidative modifications to aconitase, creatine kinase, or ATP synthase—and does not establish that those three enzymes explain decreased ATP production. (LLM, conf=0.87)

    Experimental — accuracy not yet measured
  • HSP70 is modified by both HNE and 3-NT in an MCI hippocampus.

    “while HSP70 is modified by both HNE and 3-NT in an MCI hippocampus [56,58]”

    From Mitochondrial Oxidative and Nitrosative Stress and Alzheimer Disease (2020) · cites this paper as [58]

    The Results identify HSPA8 (HSP70) as nitrated in MCI hippocampus and report an anti-3NT-positive spot at its position. The Discussion reports increased protein-bound HNE in MCI hippocampus generally, but does not identify HSPA8 as HNE-modified. Page numbers were not available in our copy. (LLM, conf=0.87)

    Experimental — accuracy not yet measured
  • 3-nitrotyrosine levels, indicating peroxynitrite damage, are elevated in Alzheimer disease and amnestic mild cognitive impairment.

    “Elevated levels of 3-nitrotyrosine (3-NT), suggestive of damage by peroxynitrite (Table 1) are also observed in AD18,24 and aMCI25.”

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Research works printed but not linked: 1

  1. [25]Chetelat G Desgranges B De La Sayette V Viader F Eustache F At the boundary between normal aging and Alzheimer disease Rev Neurol 2004 160 S55 63 15118553

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Authors
Sultana, R., Reed, T., Perluigi, M., Coccia, R., Pierce, W. M., & Butterfield, D. A.
Affiliations
  • University of Kentucky (US)
  • University of Louisville (US)
Corresponding author
Butterfield, D. Allan
Venue
Journal of Cellular and Molecular Medicine
Publisher
Wiley
Published
2007-06-24
Volume
11
Issue
4
Pages
839-851
ISSN
  • 1582-1838 (ISSN-L)
  • 1582-4934
OpenAlex
W2052209709
Cited by
87
References
82
Citations by year
2025: 3 · 2024: 1 · 2023: 4 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 6 · 2017: 1 · 2016: 4 · 2015: 9 · 2014: 2 · 2013: 5 · 2012: 4
FWCI
7.58
Type
journal article
Language
en
Open access
Locations
  • Journal of Cellular and Molecular Medicine (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • UKnowledge (University of Kentucky) (submittedVersion)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
Funders
  • University of Kentucky
  • National Institute on Aging (P01AG010836, P01AG005119, AG 10836, AG0 5119)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
nitrated proteins, amnestic mild cognitive impairment, oxidative stress, Alzheimer's disease, hippocampus, inferior parietal lobule, protein nitration, brain proteomics, disease progression
MeSH terms
Aged, 80 and over, Amnesia, Brain, Cognition Disorders, Female, Hippocampus, Humans, Male, Nerve Tissue Proteins, Nitrates, Mass Spectrometry, Reproducibility of Results, Proteomics

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