Demonstration of Amyloid Deposits and Neurofibrillary Changes in Whole Brain Sections

Braak, Heiko; Braak, Eva

doi:10.1111/j.1750-3639.1991.tb00661.x

Cited by 598Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchAmyloidosis: Diagnosis, Treatment, Outcomes

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Demonstration of Amyloid Deposits and Neurofibrillary Changes in Whole Brain Sections

Braak, Heiko, Braak, Eva·1991·Brain Pathology

DOI 10.1111/j.1750-3639.1991.tb00661.x

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Abstract

Selective and sensitive silver staining of extracellular amyloid deposits and intraneuronal neurofibrillary changes can be applied to 50–150 μ.m thick polyethylene glycol sections and/or 5–15 μ.m thick paraffin sections. The silver techniques take advantage of physical development of the nucleation sites thus permitting tight control of the entire procedure. Both techniques can be applied to routinely fixed autopsy material. They do not require particular skills and considerably facilitate processing of large numbers of sections through entire hemispheres of the human brain.

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Both amyloid and neurofibrillary changes begin during preclinical AD when cognitive deficits are not apparent.
“Importantly, both amyloid and neurofibrillary changes begin during preclinical AD when cognitive deficits are not apparent (8).”
From The Inflammatory Continuum of Traumatic Brain Injury and Alzheimer’s Disease (2018) · cites this paper as [8]

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Research works printed but not linked: 14

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Authors: Braak, H., & Braak, E.
Affiliations: Goethe University Frankfurt (DE)
ORCID: Braak, Heiko
Venue: Brain Pathology
Publisher: Wiley
Published: 1991-04
Volume: 1
Issue: 3
Pages: 213-216
ISSN: 1015-6305 (ISSN-L)
1750-3639
DOI: 10.1111/j.1750-3639.1991.tb00661.x
OpenAlex: W2131478918
Cited by: 598
References: 0
Citations by year: 2026: 12 · 2025: 18 · 2024: 39 · 2023: 22 · 2022: 20 · 2021: 35 · 2020: 38 · 2019: 28 · 2018: 26 · 2017: 21 · 2016: 24 · 2015: 25 · 2014: 25 · 2013: 24 · 2012: 24
FWCI: 2.90
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor, 6146 d)
Locations: Brain Pathology (publishedVersion)
PubMed (publishedVersion)
Funders: Deutsche Forschungsgemeinschaft
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
Health Sciences › Medicine › Internal Medicine › Amyloidosis: Diagnosis, Treatment, Outcomes
Keywords: amyloid deposits, silver staining, extracellular amyloid, paraffin sections, human brain, autopsy material, nucleation sites
MeSH terms: Alzheimer Disease, Amyloid, Brain, Brain Chemistry, Humans, Silver Staining, Neurofibrillary Tangles

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Demonstration of Amyloid Deposits and Neurofibrillary Changes in Whole Brain Sections

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